This study was conducted in the grape vineyard of Department of Horticulture and landscape design/College of Agriculture and Forestry/University of Mosul on Thompson Seedless and Olivette noir grape cultivars (Vitis vinifera L.) at three years old, by using Split-plot in Randomized Complete Block Design (RCBD) with three factors, three replications and two vines for each experimental unit, First factor was grape cultivars (Thompson Seedless and Olivette noir), second factor was inoculation with Bacillus
subtilis bacteria with three levels (0, 20 and 40 g vine-1) third factor was Trichoderma
harzianum fungi with three levels (0, 20 and 40 g vine-1) too, in addition to control treatment (without inoculation), as the inoculation with the bacterial and fungus was carried out once on a season according to the treatments in 18 March. To study the effect of each factor separately and the interactions between factors. These fertilizers were used to improving the vegetative growth. The obtained results indicated that application of 40g vine-1 of Bacillus subtilis and Trichoderma harzianum on Thompson Seedless and Olivette noir cultivars individually or interaction with each other caused a significant increase in the branches length, leaf area, leaves area of vine, total carbohydrate in the leaves and dry weight of leaves, while application of 20 g vine-1 of Bacillus subtilis + 40 g vine-1 of Trichoderma
harzianum of Olivette noir grape cultivar gave the highest value for the total chlorophyll content in leaves.