A field experiment was conducted during kharif season of the year 2018-19 at farm, Department of Agronomy, College of Agriculture, VNMKV, Parbhani to study the effect of plant growth regulators and fertilizer spray on growth and yield of Niger [Guizotia abyssinica (L.f.) Cass]." The investigation was carried out in RBD design with three replication and ten foliar application of plant growth regulators and fertilizers such as T1-19:19:19 (1%), T2 -Urea (1%) + DAP (1%), T3 -DAP (2%), T4 -Urea(2%), T5 -Brassinosteroids (0.05%), T6 -NATCA (50ppm), T7 -NAA (20ppm), T8-GA3(20ppm), T9 -Nitrobenzene (400ppm), T10 -Control (no spray). The result of experiment revealed that among the treatments foliar application of Brassinosteroids (0.05%) (T5) to Niger at bud initiation stage recorded significantly higher growth, yield attributes and yield as compare to other treatments and which was at par with foliar application of NATCA(50PPM) and nitrobenzene(400PPM).