Effect of Follicular Fluid Oxidative Stress on Meiotic Spindle Formation in Infertile Women with Polycystic Ovarian Syndrome

Chattopadhayay, Ratna; Ganesh, Ashalatha; Samanta, Jayeeta; Jana, Saikat Kumar; Chakravarty, B.; Chaudhury, Koel

doi:10.1159/000270900

Cited by 88 publications

(61 citation statements)

References 51 publications

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“…There is inconsistent data about lipid peroxidation in IVF patients. In a study by Chattopadhayay et al, increased MDA concentrations, as an index of lipid peroxidation were observed [29]. In contrast to this study, Prieto et al found no difference between women with endometriosis compared with control group [30].…”

Section: Discussioncontrasting

confidence: 70%

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Pekel

Gönenç

Turhan

et al. 2014

J Assist Reprod Genet

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Purpose The Fas-Fas Ligand interaction is one of the essential events for the induction of apoptosis whereas the exact role of their soluble forms in the reproductive system is still not fully understood. Also oxidative stress in the pathogenesis of infertility causing diseases in women and has been suggested as one of the important factors that negatively affect IVF outcome. In this study, our aim was to evaluate serum and follicular fluid levels of soluble Fas soluble Fas Ligand, malondialdehyde, superoxide dismutase and total antioxidant capacity in patients undergoing IVF and compared with controls. Methods This study included 109 patients. Patients were classified as unexplained infertility (N=31), PCOS (N=19), tubal factor (N=9) and endometriosis (N=10) and compared with male factor infertility (N=40) that was the control group. sFas and sFasL levels were measured by immunoassay method. MDA, SOD and TAC levels were measured by colorimetric method. Results Patients with unexplained infertility, PCOS and tubal factor had significantly lower sFas levels compared with their controls (respectively, p<0.01, p<0.05, p<0.05). However, SOD activity in unexplained infertility, PCOS and endometriosisgroupswere significantly higher than control group (p<0.01).Decreased follicular fluid TAC levels were found in all patient groups compared with controls (respectively, p<0.01, p<0.05, p<0.01, p<0.01).Patients with tubal factor had significantly higher serum sFasL (p<0.05), but lower follicular fluid sFasL levels (p<0.05) compared with unexplained infertility. Tubal factor and endometriosis groups had lowerfollicular fluid TAC levels compared to unexplained infertility and PCOSgroups (p<0.01). Conclusion(s) In this study, serum and follicular fluid sFas levels were decreased and antioxidant activity was impaired in infertility, possibly implying increased apoptosis. Especially in unexplained infertility group changes in this parametres more remarkable.

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Section: Discussioncontrasting

confidence: 70%

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Pekel

Gönenç

Turhan

et al. 2014

J Assist Reprod Genet

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“…In addition, follicular fluid OS levels effecting the meiotic spindle formation in the oocyte was suggested as the related cause of infertility in PCOS cases [20]. Supporting this data, in PCOS cases, significantly higher levels of FF ROS and lower levels of FF TAC were recorded in the absence of meiotic spindle formation [21]. As previously suggested elevated ROS in the FF tends to limit the fertilization potential of oocytes, through disruption of meiotic spindle formation.…”

Section: Discussionsupporting

confidence: 57%

The ımpact of follicular fluid ıschemia-modified albumin levels on ICSI outcomes ın PCOS patients

Duzguner¹,

Taşçı²,

Çağlar³

et al. 2017

J Pregnancy Reprod

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Aim: Serum and follicular fluid ischemia-modified albumin levels and total antioxidant capacity were evaluated in patients with polycystic ovary syndrome applied ICSI-ET to clarify the association between these markers and IVF outcome parameters. Methods:In this prospective controlled clinical trial, the study group consisted of PCOS cases (n=30) undergoing ICSI-ET cycles. The control group consisted normogonadotropic female partners of male factor infertility (n=30). The controlled ovarian hyperstimulation was performed by long protocol down regulation and recombinant FSH stimulation. Aspirated follicular fluid from the first retrieved follicle were analyzed for TAC and IMA levels. Oocyte quality, fertilization rate, embryo quality and final pregnancy outcomes were assessed.Results: No statistically significant difference was found between the groups when compared for mean age, body mass index and cycle outcome parameters (p>0.05). FF IMA levels were found positively correlated with embryo grading (r=0.328; p=0.03). The sensitivity, specificity, positive and negative predictive values of the best cutoff value of FF IMA (1.475 ABSU) for the prediction of grade 1 embryo development were 75%, 72%, 73% and 60%, respectively (AUC:0.765;CI 95%:0,606-0,924; p=0.017). In PCOS cases with TAC ≤1.299 mmol/L compared with TAC >1.299 mmol/L, number of metaphase II oocytes, fertilization rates, embryo grading and FF IMA levels were found significantly different (p<0.05). Conclusions:The levels of IMA and TAC in FF were higher than serum levels which inturn indicates a greater degree of oxidative stress in follicular microenvironment compared to systemic circulation. Although oxidative stres in FF of PCOS cases was found similar to non-PCOS cases when detected by IMA, a significant positive correlation between FF IMA levels and embryo grade may indicates the role of augmented oxidative stress in infertility.

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“…Some studies have consistently found increased concentration of OS markers in the follicular fluid (FF) from obese or PCOS patients when compared to controls and a relationship between increased OS level and poor outcome after in vitro fertilization has been reported [4,5]. It has been extensively reported that the effective reduction of insulin resistance obtained with metformin restores regular menstrual cycles, ovulation and improves fertility [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

Piomboni

Focarelli

Capaldo

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the oxidative stress status (OS) of follicular fluid (FF) and the oocyte quality in women with polycystic ovary syndrome (PCOS) undergoing different ovarian stimulation protocols. Methods FF samples were collected after gonadotropin administration in association or not with metformin or D-chiro-inositol (DCI). OS status was then evaluated by checking the follicular fluid protein oxidation profile after specific labeling of aminoacidic free-SH groups, and two-dimensional electrophoresis followed by qualitative and semiquantitative analysis. Oocyte quality was assessed by international morphological criteria. Results Our data indicated that both treatments, even if to different extent, recovered a significantly high level of free-SH groups in FF proteins of PCOS women clearly indicating a decrease of OS level with respect to that found in FF samples from gonadotropins alone treated women. A higher number of good quality MII oocytes was also observed in DCI (P<0.05) or metformin (P<0.05) study groups in comparison to untreated control group. Conclusion A natural supplement and a drug both showed a statistically significant positive effect on follicular milieu by decreasing the oxidative damage on FF proteins, as well as in recovering good quality oocytes.

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Effect of Follicular Fluid Oxidative Stress on Meiotic Spindle Formation in Infertile Women with Polycystic Ovarian Syndrome

Cited by 88 publications

References 51 publications

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

Changes of sFas and sFasL, oxidative stress markers in serum and follicular fluid of patients undergoing IVF

The ımpact of follicular fluid ıschemia-modified albumin levels on ICSI outcomes ın PCOS patients

Protein modification as oxidative stress marker in follicular fluid from women with polycystic ovary syndrome: the effect of inositol and metformin

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