Effect of follicular size on meiotic and developmental competence of porcine oocytes

Marchal, R.; Vigneron, C.; Perreau, C.; Bali-Papp, A.; Mermillod, Pascal

doi:10.1016/s0093-691x(02)00655-6

Cited by 155 publications

(120 citation statements)

References 20 publications

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“…Confirming previous studies (MARCHAL et al, 2002), the oocytes from follicles with 1-3 or 4-6mm in diameter had lower (P<0.05) maturation rates than those from 6-8 or >8mm in diameter ( Figure 1A). Initially, Grb10 mRNA expression was evaluated in isolated oocytes and cumulus cells by RT-PCR assay in order to validate the model.…”

Section: Grb10 Mrna Expression and Nuclear Maturation Rates In Cocs Fsupporting

confidence: 90%

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes

et al. 2015

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Section: Grb10 Mrna Expression and Nuclear Maturation Rates In Cocs Fsupporting

confidence: 90%

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes

et al. 2015

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“…The difference in maturation rates between FSH and eCG treatments may be attributed to the difference in the follicular response after treatment. It has been demonstrated that larger-diameter follicles contain oocytes with higher meiotic or developmental potential in pigs (Motlik et al 1984, Luca et al 2002, Marchal et al 2002, cattle (Lonergan et al 1994, Blondin & Sirard 1995, Lequarre et al 2005 and monkeys (Gilchrist et al 1997). The ability of the oocyte to develop to the blastocyst stage is thought to be a suitable indication of competence.…”

Section: Discussionmentioning

confidence: 99%

“…However, these recovered oocytes did not reach the blastocyst stage when they were matured and fertilized in vitro and immediately transferred to the oviducts of estrous-synchronized recipients gilts (Kikuchi et al 2006). According to findings obtained in prepubertal gilts, oocytes isolated from antral follicles at least 2 mm in diameter resume meiosis at a higher rate than those from smaller follicles (Motlik et al 1984), and oocytes from follicles between 3 and 5 mm in diameter have been proven to acquire the ability to develop to the blastocyst stage in vitro (Kikuchi et al 2002, Marchal et al 2002. On the other hand, the antral follicles in xenografts examined 48 h after eCG injection did not exceed 2 mm in our previous study (Kaneko et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice

Kaneko¹,

Kikuchi²,

Noguchi³

et al. 2006

Reproduction

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Our objective was to improve the developmental ability of oocytes in porcine primordial follicles xenografted to nude mice, by treating the host mice with gonadotrophins to accelerate follicular growth. Ovarian tissues from 20-day-old piglets, in which most of the follicles were primordial, were transplanted under the kidney capsules of ovariectomized nude mice. Gonadotrophin treatments were commenced around 60 days after vaginal cornification in the mice. Ovarian grafts were obtained 2 or 3 days after treatment with equine chorionic gonadotrophin (eCG-2 and eCG-3 groups), after porcine FSH infusion for 7 or 14 days, or after infusion of porcine FSH for 14 days with a single injection of estradiol antiserum (FSH-7, FSH-14 and FSH-14EA groups, respectively). Gonadotrophin treatments accelerated follicular growth within the xenografts compared with that in control mice given no gonadotrophins, consistent with higher (P < 0.05) circulating inhibin levels in the gonadotrophin-treated mice. In contrast, circulating mouse FSH levels were significantly (P < 0.05) depressed. We recovered large numbers of full-sized oocytes with meiotic competence to the mature stage from the eCG-3, FSH-7, and FSH-14EA, unlike in the control group. Moreover, 56% of matured oocytes with the first polar body (n 5 39) were fertilized in vitro in the FSH-14EA group. After in vitro fertilization and subsequent culture for 7 days, one blastocyst was obtained from each of the eCG-3, FSH-7 and, FSH-14EA groups, whereas no blastocysts appeared in the other groups. Exogenous gonadotrophinsnot mouse FSH -stimulated the growing follicles that had developed from the primordial follicles in the xenografts: the effects were incomplete but improved to some extent the meiotic and developmental abilities of the oocytes.

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“…It has been documented that the size of follicles from which oocytes are derived has a significant effect on the quality of oocytes, their maturation, fertilization and development both in pigs (Bolamba and Sirard, 2000;Marchal et al, 2002;Krisher, 2004;) and in cattle (Raghu et al, 2002). Factors within follicles, at later stages of their development, play an important role during oocyte maturation (Algriany et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Porcine oocytes from follicles smaller than 3 mm are not fully meotically competent, because they are cytoplasmically deficient (Sun et al, 2001;Marchal et al, 2002). They are also smaller in size and show a lower glutathione content before and after maturation (Liu et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro

Machatkova¹,

Hulinska²,

Horakova³

et al. 2008

Vet. Med.

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ABSTRACT:The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5-9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1-5), middle (6-10) and late (11-14) luteal or early and late (20-21) follicular phase. Oocytes were isolated separately from medium (5-9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least two compact layers of cumulus cells were selected as useful for maturation. They were matured by a standard protocol, denuded from cumulus cells, fixed in glutaraldehyde, stained with 33258-Hoechst and examined by epifluorescence. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competence regardless of the phase. The mean number (± S.E.M.) of oocytes isolated per donor was higher (187.7 ± 48.4 vs. 16.9 ± 6.1) but the mean percentage (± S.E.M.) of useful oocytes was lower (22.4 ± 7.5% vs. 80.2 ± 6.8%; P < 0.01) for small than for medium follicles. The mean number (± S.E.M.) of useful oocytes per donor was significantly (P < 0.01) higher (42.1 ± 16.8 vs. 11.9 ± 4.3) but the mean percentage of matured oocytes was significantly (P < 0.01) lower (48.4 ± 17.8% vs. 79.9 ± 7.9%) for small than for medium follicles. The oocyte population collected from small follicles varied during the oestrous cycle. The mean number (± S.E.M.) of oocytes isolated per donor from small follicles increased during the luteal and decreased during the follicular phase, except for the late follicular phase when it increased again. The mean percentage (± S.E.M.) of useful oocytes did not differ too much during this period, except for the late follicular phase when it decreased significantly (P < 0.01). The mean number (± S.E.M.) of useful oocytes per donor increased during the luteal and decreased during the follicular phase, but the differences were not significant except for the late follicular phase (P < 0.01). Similarly, the mean percentage (± S.E.M.) of matured oocytes increased during the luteal and decreased during the follicular phase, and the differences were significant. Compared with the oocyte population from small follicles, the oocyte population from medium follicles was less variable in the period from the middle luteal to middle follicular phase, when these follicles were present on the ovaries. It can be concluded that the porcine oocyte population changes in terms of quantity, morphological quality and meiotic competence according to the stage of follicular development. The late luteal and early follicular phases appeared to be most productive for oocyte recovery, because more morphologically normal oocytes with greater meiotic competence were collected, as compared with the other stages of the oestrous cycle.

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Effect of follicular size on meiotic and developmental competence of porcine oocytes

Cited by 155 publications

References 20 publications

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes

Grb10 characterization in bovine cumulus oocyte complexes from different follicle sizes

Effects of gonadotrophin treatments on meiotic and developmental competence of oocytes in porcine primordial follicles following xenografting to nude mice

Oestrous cycle stage influences the morphology and maturation of porcine oocytes in vitro

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