Effect of freeze dried bovine amniotic membrane extract on full thickness wound healing

Abstract

This study explored the feasibility of development of solubilized amniotic membrane extract (AME) as a potential wound healing substrate with improved efficacy. Bovine amniotic membrane was extracted using a mixture of acetic acid and 2-mercaptopropionic acid under sonication, which was followed by the frozen, and then lyophilized processes. The effects of AME on cell migration and growth properties were evaluated from 0 to 24 h of post injury using primary human foreskin fibroblast monolayer culture with one line scratch as an in vitro wound model. Its wound healing efficacy and scar preventive effects were investigated using whole thickness biopsy punch (8 mm) wound model obtained from rabbit ear. Intra dermal injections of AME fluid (10 μl of 1.2 μg/μl) on four wound sites were performed at 1 h pre injury, post 1, 2 and 3 day. The processes and levels of re-epithelialization and dermal regeneration were examined through histological assessment with H-E staining. In cell migration study conducted at 24 h post injury, AME (1.7 μg/ml) treated cells significantly increased wound closure with 54.9 % compared to control. Histological image analysis on AME treated wound sites at 36 days post injury showed properly developed epidermal basal cell layers and weave-like dermal collagen bundles, whereas those of untreated control skin showed over-proliferation of epidermis and aggregated collagen bundles with defected dermal regeneration. The results of this study verified the feasibility of dermal injections of freeze dried AME as a potential wound healing substrate which can promote epidermal and dermal regeneration, while avoiding undesirable hyper-proliferation of damaged tissue.

Biomaterials and Nanotherapeutics for Enhancing Skin Wound Healing

“…The porcine small intestine submucosa that has been lyophilized and sterilized has been recently shown to provide similar benefits to those provided by fish skin-based products for enhancing healing dermal wounds (Mostow et al, 2005). Similarly, lyophilized bovine amniotic membrane has been effective in wound healing applications (Kang et al, 2013). The silk protein, fibroin, is an effective scaffolding material providing a fine mesh for the cells to grow and enhance wound healing (Hasatsri et al, 2015).…”

Pharmaceutical application of frog skin on full-thickness skin wound healing in mice

“…Studies using lyophilized biological medications like amnion and porcine skin demonstrated that one of their important advantages was obviating the need for frequent painful dressings associated with bleeding and which sometimes requires general anesthesia, and also, these lyophilized biological dressings are more economical, easy to handle and apply (Gajiwala & Gajiwala, 2004;Hosseini et al, 2009). Moreover, Kang et al showed that the lyophilization method can keep the advantages of fresh bovine amniotic membrane in the wound healing process (Kang et al, 2013).…”

Preparation of placental tissue transplants and their application in skin wound healing and chosen skin bullous diseases ‐ Stevens‐Johnson syndrome and toxic epidermal necrolysis treatment

“…Their effectiveness was confirmed both in experimental studies and clinical trials. Finally, some commercial amnion‐derived products have been developed for ophthalmic use, for example, Amniotic Cytokine Extract (ACE), Regener‐Eyes, Optiserum and PDA‐AF …”

“…Finally, some commercial amnion-derived products have been developed for ophthalmic use, for example, Amniotic Cytokine Extract (ACE), Regener-Eyes, Optiserum and PDA-AF. 58,[68][69][70][71][72][73] Amnion transplants are also administrated (among others) on cut skin wounds, post-excision wounds, bullous skin diseases, pressure ulcers, wounds arising near or around the nervous tissue, on damages to the mouth and eye surface.…”