Effect of freeze–thaw process on physical properties, microbial activities and population structures of anaerobic sludge

Phalakornkule, Chantaraporn; Nuchdang, Sasikarn; Khemkhao, Maneerat; Mhuantong, Wuttichai; Wongwilaiwalin, Sarunyou; Tangphatsornruang, Sithichoke; Champreda, Verawat; Kitsuwan, Jiraprat; Vatanyoopaisarn, Savitri

doi:10.1016/j.jbiosc.2016.11.005

Cited by 29 publications

(13 citation statements)

References 25 publications

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“…Thawing-freezing is critical for bacterial viability and for decreasing their recoverability. The effect of this process must be considered in the study design, data analysis and interpretation of the results (Phalakornkule et al, 2017). In our study, fecal samples showed a higher diversity of colistin-resistant bacteria when tested prior to freezing, and a significant decrease was confirmed in both occurrence and diversity after freezing.…”

Section: Discussionmentioning

confidence: 64%

“…The freezing process could have a negative effect on the viability of bacteria present in cecal samples, which may affect retrospective studies based on the detection of bacteria using culture methods. In this study, we use freezing in glycerol to sample storage, a process involving physical and chemical changes that could affect their biological composition (Phalakornkule et al, 2017). Thawing-freezing is critical for bacterial viability and for decreasing their recoverability.…”

Section: Discussionmentioning

confidence: 99%

“…Temperature and time of storage are the most critical factors when a microbiological study is carried out with frozen material. In addition, the freeze-thaw process could be an important issue for bacterial recovery since it could dramatically affect bacterial survival because of the physical changes in the frozen material, assuming that at least a 74% decrease in cell viability may occur (Phalakornkule et al, 2017). This is of paramount importance when the aim is testing bacterial diversity of frozen samples, as previous studies have demonstrated that the freeze-thaw process can decrease bacterial diversity in the samples (Phalakornkule et al, 2017;Dorsaz et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the freeze-thaw process could be an important issue for bacterial recovery since it could dramatically affect bacterial survival because of the physical changes in the frozen material, assuming that at least a 74% decrease in cell viability may occur (Phalakornkule et al, 2017). This is of paramount importance when the aim is testing bacterial diversity of frozen samples, as previous studies have demonstrated that the freeze-thaw process can decrease bacterial diversity in the samples (Phalakornkule et al, 2017;Dorsaz et al, 2020). Therefore, the use of traditional methods, such as solid or liquid culture, could be challenging and may not provide representative data on the amount and diversity of bacteria in these samples prior to being frozen.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

et al. 2020

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

et al. 2020

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“…Thus, pretreatment methods were adopted to accelerate the hydrolysis rate, which included acid, alkaline, enzymatic, and other physical methods. Among these methods, freezing-thawing (F/T) is recognized as an efficient treatment with less energy intensive, especially in the cold-climate regions [1,2]. Many studies have demonstrated the effect of sludge disruption, solubilization of organic matter, and improvement of dewaterability via this treatment [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Combined freezing-thawing pretreatment and microbial electrolysis cell for enhancement of highly concentrated organics degradation from dewatered sludge

Jia

Cheng

et al. 2020

Bioengineered

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2020) Combined freezing-thawing pretreatment and microbial electrolysis cell for enhancement of highly concentrated organics degradation from dewatered sludge, Bioengineered, 11:1, 301-310, ABSTRACTThe influence of freezing-thawing (F/T) pretreatment on the degradation of highly concentrated organic matters from dewatered sludge (DS) in microbial electrolysis cell (MEC) was investigated in this study. Extended freezing disintegrated the DS matrix and resulted in accelerated hydrolysis rate. The biogas production and stabilization were increased due to the pretreatment by 25-70% of H 2 production rate and 17.8-33.8% of COD reduction rate, respectively. Fourier transform infrared spectroscopy analysis indicated that the pretreatment was unable to alter the bioelectrochemical reactions except for accelerating degradation rate. Excitation and emission matrix (EEM) spectra showed that aromatic protein and soluble microbial products (SMPs)like materials in DS were increasingly solubilized by the pretreatment and significantly removed during electrogenesis. The F/T-pretreated DS favored the enrichment of exoelectrogens in MEC. ARTICLE HISTORY

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Effects of freezing–thawing pretreatment on anaerobic digestion of wheat straw and its kinetics analysis

Zhang

Yao

2021

Clean Techn Environ Policy

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In this study, freezing-thawing (FT) pretreatment of different freezing time and freezing temperatures was investigated to nd the effect on anaerobic digestion of wheat straw (WS). The freezing temperature gradient is -10, -20, -40 and -80℃, and the freezing time gradient is 12 h, 24 h, 48 h and 96 h. Total methane production exhibited a mere distance among all samples. Morphology change sculptured by SEM showed this method broken the structure of WS leaving fragments and pores in varying degrees. Three kinetic models were performed on WS to represent the behavior of experimental data. Kinetic model parameters of total methane production and lag phase time showed that logistic function model had the best t, followed by modi ed Gompertz model, yet transfer function model lost e cacy in this experiment. Logistic function model was then used to reveal the in uence on lag phase caused by freezing time and freezing temperature, the results implied that FT pretreatment can shorten the lag phase time of anaerobic digestion (AD), providing a 21.39% improvement under the optimal conditions of -20℃ 96 h. The analysis of response surface regression shows that the freezing temperature has more effect on the lag phase time of anaerobic digestion than freezing time. Warmer freezing temperature of -20℃ do better than -80℃ on lag time, which can be achieved in most cold regions, so this treatment can occur naturally in such area without additional energy input.In this study, freezing-thawing (FT) pretreatment of different freezing time and freezing temperatures was investigated to nd the effect on anaerobic digestion of wheat straw (WS). The freezing temperature gradient is -10, -20, -40 and -80℃, and the freezing time gradient is 12 h, 24 h, 48 h and 96 h. Total methane production exhibited a mere distance among all samples. Morphology change sculptured by SEM showed this method broken the structure of WS leaving fragments and pores in varying degrees. Three kinetic models were performed on WS to represent the behavior of experimental data. Kinetic model parameters of total methane production and lag phase time showed that logistic function model had the best t, followed by modi ed Gompertz model, yet transfer function model lost e cacy in this experiment. Logistic function model was then used to reveal the in uence on lag phase caused by freezing time and freezing temperature, the results implied that FT pretreatment can shorten the lag phase time of anaerobic digestion (AD), providing a 21.39% improvement under the optimal conditions of -20℃ 96 h. The analysis of response surface regression shows that the freezing temperature has more effect on the lag phase time of anaerobic digestion than freezing time. Warmer freezing temperature of -20℃ do better than -80℃ on lag time, which can be achieved in most cold regions, so this treatment can occur naturally in such area without additional energy input.This study investigated the effect of FT pretreatment on AD of WS. This pretreatment method is novel and has been less investigate...

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Effect of freeze–thaw process on physical properties, microbial activities and population structures of anaerobic sludge

Cited by 29 publications

References 25 publications

Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

Complementarity of Selective Culture and qPCR for Colistin Resistance Screening in Fresh and Frozen Pig Cecum Samples

Combined freezing-thawing pretreatment and microbial electrolysis cell for enhancement of highly concentrated organics degradation from dewatered sludge

Effects of freezing–thawing pretreatment on anaerobic digestion of wheat straw and its kinetics analysis

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