Effect of fructo-oligosaccharide supplementation in soya beverage on the intestinal absorption of calcium and iron in newly weaned rats

Abstract

Studies have shown the positive effects of prebiotics on the intestinal absorption of Ca and Fe. The present study evaluated the effect of fructo-oligosaccharide (FOS) supplementation in soya beverage (SB) on absorption mechanisms of Ca and Fe in recently weaned rats. Male Wistar rats were divided into four groups: lactose-free cows’ milk (CM), lactose-free CM with FOS (0·8 g/100 ml) (CMF), SB and soya beverage with FOS (0·8 g/100 ml) (SBF). These rats were euthanised after 1 week of treatment. Organ weight, pH of the caecal content and absorption mechanisms of Ca and Fe were evaluated. The results showed that the weight of the caecal contents increased in the CMF and SBF groups, and the pH of the caecal contents was lower in these groups. The Hb levels of the CMF and SB groups were higher when compared with that of the CM group and lower in relation to the SBF group. The apparent Ca and Fe absorption and apparent Ca retention in the CM group were higher when compared with the SB group, whereas in the CMF group, they were higher in relation to the SBF group. Divalent metal transporter 1 (DMT1) protein expression in the duodenum was higher in the SBF group than in the SB and CMF groups. SB resulted in lower intestinal Ca absorption and higher Hb concentration, despite the lower apparent Fe absorption in relation to CM. Supplementation with FOS provided beneficial effects on Hb and DMT1 protein expression in the duodenum, in addition to improving the absorption process.

Prebiotic Oligosaccharides Enhance Iron Absorption Via Modulation of Protein Expression and Gut Microbiota in a Dose‐Response Manner in Iron‐Deficient Growing Rats

“…In addition, in line with our findings, a previous study found that FOS supplementation increased apparent iron absorption in a newly weaned rat model. [36] In agreement with results from Lobo et al (2011) [12] in which ID rats were supplemented with 7.5% inulin-type fructans, hepatic iron levels in ID rats supplemented with 10% GOS or 10% FOS in the present study were recovered to the comparable level of the normal group but not that of the ID-Control, ID-3% GOS, or 5% GOS groups, suggesting that higher dose of GOS was more effective in restoring iron status.…”

“…In addition, in line with our findings, a previous study found that FOS supplementation increased apparent iron absorption in a newly weaned rat model. [ 36 ]…”

“…[ 24 ] Rats supplemented with FOS in soya beverages maintained higher levels of DMT1 in duodenum than those with FOS in lactose‐free cow's milk or without FOS. [ 36 ] Supplemental inulin enhanced the expression of iron‐binding/transport proteins (DMT1 in the cecum, ferroportin in the duodenum) in a piglet model. [ 9 ] FOS supplementation decreased ferroportin expression in cecal mucosa in an iron‐deficient rat model.…”

Assessing the Nutritional Value of Root and Tuber Crops from Bolivia and Peru

“…Yacon, as of now considered a novel food after fulfilling safety standards, is already being produced and marketed for controlling bodyweight or dietary supplement in hyperglycemic patients [57,58,59]. The use of yacon syrups or inclusion of yacon flour in fermented milks has beneficial effects when adopted in healthy diets by improving organoleptic properties of dairy products [59,60,61] and the intestinal absorption of Ca and Fe [62]. Even though other compounds, like mashua glucosinolates, might provide nutraceutical properties on prostate affections attributed by folk medicine [63], they should await further studies involving humans.…”

The arsenic-lowering effect of inulin-type prebiotics in end-stage renal disease: a randomized crossover trial

“…28,29 Moreover, divalent metal transporter 1 (DMT1) in the intestinal epithelium has been reported to be upregulated by inulin. 30 On the other hand, inulin stimulates symbiotic bacteria, especially Bifidobacterium and Lactobacillus, which eliminate toxic elements by directly chelating with the carboxyl group of proteins and hydroxyl group of the peptidoglycans in the cell membrane. 31 In addition, accelerating the enterohepatic circulation of bile acids (BAs) by manipulating the gut microbiota has also been reported to enhance the biliary secretion of heavy metals into the intestine.…”