Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature

Abstract

This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5oC) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p <0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.

Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C

“…The concentration of Sapudi ram's fresh semen in this study was 1,940.00 ± 259.98 million/mL. This is in line with previous research, wherein Sapudi ram spermatozoa has a concentration of around 1,500-3,000 million/mL (Puspita et al, 2020;Retta et al, 2022). Fresh semen for artificial insemination must be of good quality, with a motility percentage of more than 70% (Quraini et al, 2022).…”

“…In this study, the semen of Sapudi ram extended in SM-GEY without GTLE maintained spermatozoa motility of more than 40% for two days. This observation suggested that the function of goose egg yolk in this study was in line with the research result of Retta et al (2022) which also maintained the motility of spermatozoa of Sapudi ram compared to the addition of skim milk extender alone or chicken egg yok-skim milk extender (Puspita et al, 2020).…”

“…The higher the LDL in the extender, the more effective it is in maintaining the integrity of the spermatozoa plasma membrane (Vera-Munoz et al, 2011). A previous report showed that the semen of Sapudi rams in skim milk extender alone (Retta et al, 2022) or chicken egg yolk-skim milk (Puspita et al, 2020), without added antioxidants, maintained more than 40% motility for two days at 5 °C. In this study, the semen of Sapudi ram extended in SM-GEY without GTLE maintained spermatozoa motility of more than 40% for two days.…”

Combination of 5% Dextrose Ringer’s solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature

“…Semen sample was dripped onto an object glass and then covered with a cover slip. Progressive spermatozoa motility was observed on 100 spermatozoa under a light microscope (Olympus BX-53) with 400x magnification (Retta et al, 2022).…”

“…The slides were examined under a light microscope with a magnification of 400x. Spermatozoa with transparent heads were counted as live spermatozoa, while spermatozoa with reddish heads were counted as dead (Retta et al, 2022) (Figure 1). Viability count was performed on 100 spermatozoa within 5 different fields.…”