Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

Abstract

7Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in 8 female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, 9 bone loss and inflammation as well. Several strategies used for treatment OA including non-10 pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, 11 antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect 12 of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate 13 ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat 14 diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered 15 with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) 16 after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and 17 hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight 18 gain and feed intake.