Effect of Fufang Xueshuantong Capsule (复方血栓通胶囊) on a rat model of retinal vein occlusion

Yuan, Yuanzhi; Yuan, Fangyan; Xu, Qin-yue; Yu, Jia-Kuo; Li, Lei; Zhang, Juli

doi:10.1007/s11655-011-0690-6

Cited by 18 publications

(22 citation statements)

References 21 publications

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“…To validate zebrafish thrombosis model for pharmaceutical testing, six marked human antithrombotic drugs aspirin (thromboxane A2 inhibitor), 13 clopidogrel (ADP inhibitor), 14 diltiazem hydrochloride injection (calcium channel blocker), 15 xuanshuantong injection (inhibiting platelet aggregation, blood coagulation, fibrinolysis activation, and reducing plasma viscosity), 17 salvianolate injection (antiplatelet aggregation, anticoagulant, and antithrombosis), 36 and astragalus injection (restraining thrombosis and platelet aggregation) 37 were chosen and tested in this model for preventive and therapeutic effects on thrombosis. All six human antithrombotic drugs significantly reduced zebrafish thrombosis after 24 h cotreatment (thrombosis prevention) or treatment (thrombus therapy), indicating that the zebrafish thrombosis model developed in this report is suitable for in vivo screening and assessment of oral and injectable antithrombotic drugs no matter as a single compound medicine or a herb extract.…”

Section: Discussionmentioning

confidence: 99%

“…16 Xueshuantong, an injectable drug extracted and prepared from an Chinese herb Panax notoginseng, has been used to treat thrombotic diseases in China for more than 30 years and has been proved highly effective. 17 Salvianolate prepared from a Chinese herb Salvia miltiorrhiza is a commonly used medicine for ''invigorating'' the blood and reducing blood clotting in eastern countries, particularly in China, and many studies have shown that salvianolate can inhibit platelet aggregation and adhesion and reduce thrombus formation. 18 Astragalus as a TCM has been used for thousands of years, and recently, mainly for treating thrombosis.…”

Section: Introductionmentioning

confidence: 99%

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A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

Zhu

Liu²,

Guo³

et al. 2016

Zebrafish

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Thrombosis is a leading cause of death and the development of effective and safe therapeutic agents for thrombotic diseases has been proven challenging. In this study, taking advantage of the transparency of larval zebrafish, we developed a larval zebrafish thrombosis model for drug screening and efficacy assessment. Zebrafish at 2 dpf (days post fertilization) were treated with phenylhydrazine (PHZ) and a testing drug for 24 h. Tested drugs were administered into the zebrafish either by direct soaking or circulation microinjection. Antithrombotic efficacy was quantitatively evaluated based on our previously patented technology characterized as an image analysis of the heart red blood cells stained with O-dianisidine staining. Zebrafish at 2 dpf treated with PHZ at a concentration of 1.5 μM for a time period of 24 h were determined as the optimum conditions for the zebrafish thrombosis model development. Induced thrombosis in zebrafish was visually confirmed under a dissecting stereomicroscope and quantified by the image assay. All 6 human antithrombotic drugs (aspirin, clopidogrel, diltiazem hydrochloride injection, xuanshuantong injection, salvianolate injection, and astragalus injection) showed significant preventive and therapeutic effects on zebrafish thrombosis (p < 0.05, p < 0.01, & p < 0.001) in this zebrafish thrombosis model. The larval zebrafish thrombosis model developed and validated in this study could be used for in vivo thrombosis studies and for rapid screening and efficacy assessment of antithrombotic drugs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Zebrafish Thrombosis Model for Assessing Antithrombotic Drugs

Zhu

Liu²,

Guo³

et al. 2016

Zebrafish

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“…Thus, FFA is recommended for studies on retinal vessel diseases. In this research, the injected concentrations and volumes of contrast agent were obtained from literature review [22][23][24][25][26] and our experimental experience. At high concentrations, signals rose faster and higher to reveal the overall vascular structure quickly and in detail.…”

Section: Discussionmentioning

confidence: 99%

Fundus Photography, Fundus Fluorescein Angiography, and Optical Coherence Tomography of Healthy Cynomolgus Monkey, New Zealand Rabbit, Sprague Dawley Rat, and BALB/c Mouse Retina

Xian

Zhao²,

Peng³

et al. 2020

Preprint

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Background A variety of experimental animal models are used in basic ophthalmological research to elucidate physiological mechanisms of vision and disease pathogenesis. The choice of animal model is based on the measurability of specific parameters or structures, the applicability of clinical measurement technologies, and the similarity to human eye function. Studies of eye pathology usually compare optical parameters between a healthy and altered state, so accurate baseline assessments are critical, but few reports have comprehensively examined the normal anatomical structures and physiological functions in these models. Methods Three cynomolgus monkeys, six New Zealand rabbits, ten Sprague Dawley (SD) rats, and BALB/c mice were examined by fundus photography (FP), fundus fluorescein angiography (FFA), and optical coherence tomography (OCT). Results Most retinal structures of cynomolgus monkey were anatomically similar to the corresponding human structures as revealed by FP, FFA, and OCT. New Zealand rabbits have large eyeballs, but they have large optic disc and myelinated retinal nerve fibers in their retina, and the growth pattern of retinal vessels were also different to the human retina. Unlike monkeys and rabbits, the retinal vessels of SD rats and BLAB/c mice were widely distributed and clear. And the OCT preformance of them were similar with human beings. Conclusion Monkey is a good model to study changes in retinal structure associated with fundus disease, rabbits are not suitable for studies on retinal vessel diseases and optic nerve diseases, and rats and mice are good models for retinal vascular diseases. These measures will help guide the choice of model and measurement technology and reduce the number of experimental animals required.

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“…It is hypothesised that as the major component in the Honghua extract is glucose, the neuroprotective effect of the same could be due to availability of energy source after ischemia (Romano, et al, 1993). Another Chinese herbal medicine -Fufang Xuehuantong capsule was also studied by Yuan et al They created a rat model for retinal vein occlusion by using laser photocoagulation and then validated the therapeutic benefits Chinese herbal medicine by quantitating the expression of various growth factors in the animal model (Yuan et al, 2011).…”

Section: Photocoagulation Of Retinal Vesselsmentioning

confidence: 99%