Effect of GA3, KNO3, and removing of basal point of seeds on germination of sweet granadilla (Passiflora ligularis Juss) and yellow passion fruit (Passiflora edulis f. flavicarpa)

Abstract

-Passiflora seeds germinate erratically presenting difficulties for their handling in a greenhouse. The effect of removing of basal point of seeds (RB) and pre-imbibition of seeds of sweet granadilla and yellow passion fruit in 50, 100, 200, and 400 mg mL -1 solutions of gibberellic acid (GA 3 ) or 0.1% KNO 3 solution was studied. The experiment was conducted in greenhouses in La Plata, Colombia. Two accessions PrJ1 and PrJ2 of sweet granadilla were evaluated. There were calculated the final percentage of germination (PG), mean germination time (MGT), and the mean germination rate (MGR). The leaf area and dry mass of seedlings were measured 22 days after sowing (das); with this data, specific leaf area and relation root/ shoot were calculated. In all cases, the highest germination percentages were achieved treating seeds with KNO 3 (89, 92, and 87% for yellow passion fruit, PrJ2, and PrJ1, respectively), but the increase in MGR (3.3 germinated seeds per day) and the decrease in MGT (16 days) were only significant for PrJ1. RB had a significant reduction of PG in all cases (28, 12, and 33% for passion fruit, PrJ2 and PrJ1, respectively). With the increase in the concentration of GA 3 , PG was reduced for two accessions of sweet granadilla, for yellow passion fruit this trend was not clear, no treatment with GA 3 showed significant differences with the control. Leaf area (24.07 cm2) and dry mass of seedlings (135 mg) were significantly higher than seeds previously treated with KNO 3 only for PrJ1.The solution of KNO 3 0,1% is recommended to improve the germination and initial growth of granadilla seedlings. Index terms: Pre-imbibition, final percentage of germination, mean germination time, mean germination rate, leaf area. EFEITO DO GA 3 , KNO 3 E O DESPONTE BASAL NAS SEMENTES EM GERMINAÇÃO DE GRANADILLA (Passiflora ligularis JUSS) E O MARACUJÁ COMUM (Passiflora edulis F. flavicarpa)RESUMO-A germinação desordenada das sementes das passifloras dificulta seu manejo em estufas. Com este trabalho, procuraron-se estudar o efeito do desponte basal (DB) e a pré-embebição das sementes de granadilla (Passiflora ligularis Juss) e o maracujá comum (Passiflora edulis f. flavicarpa) submetidos a quatro concentrações de ácido giberélico (GA 3 ): 50; 100; 200 e 400 mg mL -1 , uma solução de KNO 3 a 0,1% e água como testemunha. O experimento foi realizado em estufas localizadas em La Plata (Colômbia). Para o caso do granadilla, os acessos PrJ1 e PrJ2 foram testados, avaliando os seguintes parâmetros: a) porcentagem da germinação final (PG); b) tempo médio de germinação (TMG); c) velocidade média de germinação (VMG); d) área foliar e massa seca das plântulas 22 dias após a semeadura. Com estes dados, a área foliar específica e a relação raiz/parte aérea foram calculadas. Em todos os tratamentos, as melhores percentagens da germinação foram alcançadas com solução de KNO 3 a 0,1% (89; 92 e 87% para o maracujá comum, PrJ1 e PrJ2, respectivamente). Somente foram significativos o aumento da VMG (3,3 sementes germinadas por di...

Nitrate- and nitric oxide-induced plant growth in pea seedlings is linked to antioxidative metabolism and the ABA/GA balance

“…This effect can be dependent on the KNO 3 concentration used as well as the mode of application. The direct application of KNO 3 in amounts ranging from 10-50 mM to seeds in petri dishes increased the germination rate in Paspalum sp and Passiflora sp (Cárdenas et al, 2013;Shim et al, 2008). In our work, KNO 3 was applied in two different ways.…”

“…The treatment of seeds with nitrogen-containing compounds, such as KNO 3 , has been linked to a higher germination rate in different plant species (Cárdenas et al, 2013;Shim et al, 2008). This effect can be dependent on the KNO 3 concentration used as well as the mode of application.…”

Investigation of dormancy and storage potential of seeds of yellow passion fruit

“…It could be withal noticed that seed preparations that included a cross-cut at the region opposite to the embryonic axis and water immersion for 24 hours (and the combinations of these factors) negatively affected seedling emergence (Table 2). Cutting the seeds reduced both the percentage and the speed of germination in other Passiflora species as well, probably due to the enhanced sensitiveness to pathogen attack caused by the injury (Cárdenas et al, 2013). Soaking in water for 24 hours, in turn, might have caused damage by imbibition, which, in combination with the incision, led to the inferior results obtained.…”

“…On that account, treatments with GA 3 have been suggested to tackle the physiological dormancy of several Passiflora species, including P. cincinnata Mast. (Zucareli et al, 2009;Moura et al, 2018), P. ligularis (Cadorin et al, 2017), P. caerulea (Hossel et al, 2018), P. edulis f. flavicarpa (Lima et al, 2009;Cárdenas et al, 2013), P. suberosa, P. morifolia, and P. tenuifila (Marostega et al, 2017). Different doses of this plant regulator are used, as seed dormancy is linked to the genotype (Santos et al, 2015).…”

Impacto De La Variabilidad Climática Y De Los Sistemas Agrarios en El Cultivo De Granadilla (Passiflora Ligularis Juss) De Oxapampa, Pasco, Perú

“…La granadilla (Passiflora ligularis Juss, Passifloraceae) es originaria de América Tropical, por lo que se puede encontrar en forma silvestre desde México hasta Colombia, Venezuela, Perú y Bolivia (Cerda & Castro, 2003;Ríos, 2012;Romero, 2012;Cárdenas et al, 2013;Becerra et al, 2014;Melgarejo, 2015;Ocampo et al, 2015). Esta especie es considerada la segunda especie del género de mayor importancia después del maracuyá (Passiflora edulis Degener).…”