Effect of galanin on growth hormone (GH) response to thyrotropin releasing hormone of rat pituitary gh-secreting adenomatous cells (GH1) in culture

Giustina, Andrea; Bonfanti, Carlo; Licini, Massimo; Stefana, Beatrice; Ragni, G; Turano, A

doi:10.1016/0024-3205(95)02258-9

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…Neuropeptide Y has a direct effect of GH release in some species of fish (76,77). In contrast, galanin can inhibit GH release (78)(79)(80). Moreover, peptides related to the neuropeptide Phe-Met-Arg-Phe-NH 2 (FMRF amide), originally described in mollusks, stimulate GH release in amphibians.…”

Section: The Hypothalamic Hormones Controlling Gh Release and Synthesismentioning

confidence: 99%

Growth Hormone Secretion: Molecular and Cellular Mechanisms and In Vivo Approaches

Anderson

Jeftinija

Scanes

2004

Exp Biol Med (Maywood)

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Growth hormone (GH) release is under the direct control of hypothalamic releasing hormones, some being also produced peripherally. The role of these hypothalamic factors has been understood by in vitro studies together with such in vivo approaches as stalk sectioning. Secretion of GH is stimulated by GH-releasing hormone (GHRH) and ghrelin (acting via the GH secretagogue [GHS] receptor [GHSR]), and inhibited by somatostatin (SRIF). Other peptides/proteins influence GH secretion, at least in some species. The cellular mechanism by which the releasing hormones affect GH secretion from the somatotrope requires specific signal transduction systems (cAMP and/or calcium influx and/or mobilization of intracellular calcium) and/ or tyrosine kinase(s) and/or nitric oxide (NO)/cGMP. At the subcellular level, GH release (at least in response to GHS) is accomplished by the following. The GH-containing secretory granules are moved close to the cell surface. There is then transient fusion of the secretory granules with the fusion pores in the multiple secretory pits in the somatotrope cell surface.

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Section: The Hypothalamic Hormones Controlling Gh Release and Synthesismentioning

confidence: 99%

Growth Hormone Secretion: Molecular and Cellular Mechanisms and In Vivo Approaches

Anderson

Jeftinija

Scanes

2004

Exp Biol Med (Maywood)

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“…being higher in young females compared to young males and significantly declining with menopause [34,35], One of our aims was to investigate whether median eminences and pituitaries of animals not previously ex posed to a significant steroid influence due to their physi ological phase of life would be responsive to exogenous p-galanin. We chose the concentration of galanin (100 nM) according to the minimum dose which had been demonstrated to have an effect both in cell culture and in perifusion systems [30,[36][37][38]. In the male rat, the galan in concentration has been determined in the portal circu lation (1 nM)and in median eminence fragments (10 nM) [39], To our knowledge, no data are available for the gal anin concentration in the portal circulation in ovine spe cies.…”

Section: Discussionmentioning

confidence: 99%

Modulation by Galanin of Growth Hormone and Gonadotropin Secretion from Perifused Pituitary and Median Eminence of Prepubertal Male Calves

Baratta

Saleri²,

Mascadri³

et al. 1997

Neuroendocrinology

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Galanin is widely distributed in the peripheral and central nervous system and has been indicated as a putative hypothalamic-hypophysiotropic hormone. This study was performed to investigate the effects of galanin on both growth (GH) and luteinizing hormones (LH) from pituitaries of young male calves. Pituitary slices (P, 500 µm in thickness) were perfused alone or coincubated with median eminence terminals (ME) in DMEM-F12 plus BSA 0.1% and antibiotics. The perifusion chambers were kept in equilibrium for 150 min, and medium samples were collected every 10 min for 240 min and stored at –20 °C until the measurement of LH and GH levels. Basal GH release increased up to 60% after galanin infusion (p < 0.01 vs. baseline levels) for 60 min in P alone; in P + ME coincubation, galanin-stimulated GH secretion was further increased by up to 200%. Basal LH release in chambers with P was significantly increased (up to 25%; p < 0.05) for 70 min after galanin infusion; P + ME coincubation showed a galanin-mediated increase in LH release of up to 50%. GH and LH responsiveness to exogenous GH-releasing hormone and gonadotropin-releasing hormone was not significantly modulated by galanin in our experimental model. In conclusion, galanin is demonstrated to have a significant stimulatory role in the secretion of GH and LH, with a combined action at both the hypothalamic and pituitary levels.

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“…Neuropeptide Y has a direct effect of GH release in some species of fish (Peng et al, 1993a,b). In contrast, galanin can inhibit GH release (Arvat et al, 1995;Giustina et al, 1996Giustina et al, , 1997. Moreover, peptides related to the neuropeptide Phe-Met-Arg-Phe-NH 2 (FM-RF amide), originally described in mollusks, stimulate GH release in amphibians.…”

Section: Ghrelinmentioning

confidence: 92%

Fusion Pores in Growth‐Hormone‐Secreting Cells of the Pituitary Gland: An AFM Study

Anderson¹,

Jena

2005

Force Microscopy

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Effect of galanin on growth hormone (GH) response to thyrotropin releasing hormone of rat pituitary gh-secreting adenomatous cells (GH1) in culture

Cited by 7 publications

References 20 publications

Growth Hormone Secretion: Molecular and Cellular Mechanisms and In Vivo Approaches

Growth Hormone Secretion: Molecular and Cellular Mechanisms and In Vivo Approaches

Modulation by Galanin of Growth Hormone and Gonadotropin Secretion from Perifused Pituitary and Median Eminence of Prepubertal Male Calves

Fusion Pores in Growth‐Hormone‐Secreting Cells of the Pituitary Gland: An AFM Study

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