Effect of gamma irradiation on nucleolar activity in root tip cells of tetraploid Triticum turgidum ssp. durum L

An Experimental Study on the Measurement of Gamma-Ray Linear Attenuation Coefficients with Transmission Factors for Ispir and Kızılcahamam Dry Bean

“…Similar determinations have been shown in different studies for products such as soybean, cowpea, and corn (Villavicencio et al, 2000;Mudibu et al, 2012;Sarker et al, 2014;Mirhabibi et al, 2016;Vahapoglu et al, 2022). The occurrence of such changes in food and the use of irradiation for mutation purposes in plant breeding studies are proof that the effect of gamma rays cannot be neglected (Well et al, 2022;Yasmin and Arulbalachandran, 2022). When genetically modified organisms (GMO) are considered, irradiation has the effect of disrupting the original structure of the product or organism, just like GMO processes.…”

The relationship of the efficiency of energy conversion into growth as an indicator for the determination of the optimal dose for mutation breeding with the appearance of chromosomal abnormalities and incomplete mitosis after gamma irradiation of kernels of Triticum turgidum ssp. durum L.

“…In Triticum monococcum (Von Well et al 2021 ) the 100 Gy and higher gamma irradiation treatments were not entangled with the control like 250 Gy and 350 Gy gamma irradiation treatments in the present study. The 100 Gy treatment differed from the control with p = 0.01 in Triticum monococcum (Von Well et al 2021 ).…”

“…In Triticum monococcum (Von Well et al 2021 ) the 100 Gy and higher gamma irradiation treatments were not entangled with the control like 250 Gy and 350 Gy gamma irradiation treatments in the present study. The 100 Gy treatment differed from the control with p = 0.01 in Triticum monococcum (Von Well et al 2021 ). The 100 Gy determination for the optimal dose for mutation breeding is in line with the suggested doses (100–200 Gy) for practical mutation breeding in Triticum monococcum L. by the FAO/IAEA.…”

“…Incomplete mitosis was scored as cells with five (Fig. 2 i) and more nucleoli in the nucleus resulting from separation of the chromatids during mitosis which then get packed in the same nucleus during interphase, leading to a nucleus in interphase with twice as much DNA (Von Well et al 2021 ). At all the irradiation doses interphase cells with incomplete mitosis were seen from 25 h after the onset of imbibition, with an increase of incomplete mitosis with increasing irradiation dose.…”

“…The number of micronuclei in interphase cells were determined by scoring 500 interphase cells. The number of incomplete mitosis were determined by scoring the number of interphase cells with five and more nucleoli in the nucleus out of 500 interphase cells (Von Well et al 2021 ). The mean number of bridges, ring chromosomes and micronuclei per 500 cells and the frequency of cells with different numbers of bridges, ring chromosomes and micronuclei were determined for the evaluations.…”