The most prevalent form of chronic dermatophytoses causing pathogenic fungus in humans is Trichophyton rubrum. Keratinocytes, the main epidermal cells, are crucial in local immune interactions because they represent the first line of defense against microbial assaults, skin In response to varied stimuli, keratinocytes express a wide range of receptors and create host defense peptides, cytokines, and chemokines, this immunological study's objective was to use an ELISA to determine the function of inflammatory interleukins (IL-8) in Trichophyton rubrum patients. The study included 30 patients with verified Trichophyton rubrum and 10individuals as a control group. Age, gender, the date of the surgery, the patient's occupation, their address, and the findings were all collected for every infected patient, the ELISA (Linked Immuno-sorbent Assay) technique was used to measure immune cells' cellular kinetics for secreting interleukin-8 were calculated And there was a significant increase in the level of significance for persons infected with interleukin-8 (p > 0.05), compared to control persons.