Effect of gamma radiation on the in vitro aggregability of WBC‐reduced apheresis platelets

Zimmermann, Robert; Schmidt, Stefan; Zingsem, J.; Glaser, Anke; Weisbach, Volker; Ruf, Andreas; Eckstein, Reinhold

doi:10.1046/j.1537-2995.2001.41020236.x

Cited by 23 publications

(33 citation statements)

References 44 publications

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“…The cellular elements of the blood are particularly sensitive to oxidative stress because their plasma membranes contain a high percentage of polyunsaturated fatty acids (PUFA) [43]. The significant decreases in WBCs count, values observed in the present study; confirm the results got by Robert et al [44]. The decrease in WBCs was attributed to depletion in factors needed for hematopoietic stem cells, differentiation and release from the bone marrow.…”

Section: Parameterssupporting

confidence: 90%

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

Km¹

2017

OMICS J Radiol

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The present study was designed to investigate the oxidative stress, which is due to the effect of low doses of gamma irradiation. Animals were divided into 6 groups, where the first group kept as control group, while 2 nd , 3 rd , 4 th , 5 th and 6 th groups were exposed to gamma ray at 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy once weekly for one month respectively. Rats were subjected to gamma radiation and nitric oxides (NO), superoxide dismutase (SOD), malondialdehyde, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and white blood cells (WBCs) were measured. SOD recorded highly significant decrease with percent change -22.7%, -25.01%, -55.01% -55.4% and -56.38% respectively in groups which were exposed to 1.5, 2, 2.5, 3 and 3.5 Gy. MDA serum level have a significant increase in groups which were exposed to successive doses of 1.5 Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 25.7% , 41.8% 59.1% 65.6% 75.8% consequently as compared to control group. Nitric oxide serum level is markedly showed highly significant increase in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively with percent change 59.69%, 74.17%, 87.1%, 120.1% and 130.6% consequently as compared to control group. ALT and AST recorded highly significant increase in rats exposure to successive doses of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. On the other hand WBCs recorded highly significant decreased in rats exposed to successive dose of 1.5, Gy, 2 Gy, 2.5 Gy, 3 Gy and 3.5 Gy respectively. WBCs recorded percent change -26.11%, -32.3%, -47.8%, -50.1% and -53.6% respectively as compared to control group.

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Section: Parameterssupporting

confidence: 90%

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

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2017

OMICS J Radiol

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“…5,538,894) and fluorochrome‐coupled antibodies to the cell surface antigens CD62 and CD41 (CD62‐PE and CD41‐FITC; Ortho Diagnostic Systems, Raritan, NJ). Samples were analyzed in a flow cytometer (Cytoron Absolute, Ortho) with software (Immunocount‐II, Ortho) 5 . The results were expressed as specific CD62+ cells in percentages.…”

Section: Methodsmentioning

confidence: 99%

“…Most preparations containing human platelets are used for IV transfusion to patients with neoplastic or gastrointestinal diseases or diseases of blood‐forming organs, in an effort to prevent or treat bleeding due to thrombocytopenia 1–3 . Recent studies of the systemic application of platelets are focused on side effects due to contaminating WBCs and strategies to prevent them by WBC reduction or irradiation 4,5 . However, platelets may also be used locally as a source of growth factors that play a fundamental role in wound and bone healing and in bone regeneration 6,7 .…”

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Different preparation methods to obtain platelet components as a source of growth factors for local application

et al. 2001

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“…Although gamma irradiation primarily targets nucleic acids of lymphocytes, which contaminate cellular blood components, the irradiation may also produce effects on nonlymphoid blood cells. These effects are small and probably without clinical significance when leukoreduced platelet (PLT) components are irradiated 4 . Gamma irradiation of plasma results in a significant but very weak activation of the coagulation and fibrinolytic system 5 .…”

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confidence: 99%

Influence of prestorage leukoreduction and subsequent irradiation on in vitro red blood cell (RBC) storage variables of RBCs in additive solution saline‐adenine‐glucose‐mannitol

et al. 2008

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BACKGROUND:There exists only very few data on in vitro and in vivo effects of gamma irradiation of red blood cells (RBCs) that have been leukoreduced by filtration before a subsequent irradiation. Reported studies reflect neither the current Food and Drug Administration (FDA) nor the European recommendations on timing of irradiation and subsequent storage. STUDY DESIGN AND METHODS:We studied 40 RBC units that were prepared from inline filtered whole blood and 40 RBC units that were filtered after component separation. All RBCs were stored in the additive solution saline-adenine-glucose-mannitol and leukoreduced on the collection day. In both groups, 20 components were irradiated on Day +14 with 30 Gy, and 20 served as nonirradiated controls. In vitro evaluation of both irradiated and nonirradiated RBC units was performed before and after irradiation on Days +1, +7, +14, +21, +28, +35, and +42 from the collection day. RESULTS: Gamma irradiation induced enhanced leakage of potassium ions and lactate dehydrogenase and an enhanced in vitro hemolysis rate in the irradiated components. However, in vitro hemolysis rate of both nonirradiated and irradiated components was remarkably lower than 0.8 percent, and the preservation of adenosine triphosphate over 42 days was satisfying. CONCLUSIONS: This study reflects the current FDA and European recommendations on timing of irradiation and subsequent storage. Our findings together with recent results of other investigations on the effect of gamma irradiation on leukoreduced RBCs allow the proposal that a storage time up to 28 days after irradiation is allowable.

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Effect of gamma radiation on the in vitro aggregability of WBC‐reduced apheresis platelets

Cited by 23 publications

References 44 publications

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

Effect of Gamma Ray on Reactive Oxygen Species at Experimental Animals

Different preparation methods to obtain platelet components as a source of growth factors for local application

Influence of prestorage leukoreduction and subsequent irradiation on in vitro red blood cell (RBC) storage variables of RBCs in additive solution saline‐adenine‐glucose‐mannitol

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