Effect of Gammalin 20 (Lindane) on Differential White Blood Cell Counts of the African Catfish, Clarias albopunctatus

Mgbenka, Bernard Obialo; Oluah, Ndubuisi Stanley; Umeike, I.

doi:10.1007/s00128-003-0157-3

Cited by 25 publications

(16 citation statements)

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“…The reduction of RBC is mainly due to development of hypoxic condition during the treatment which intern leads to increase in destruction of RBC or decrease in rate of formation of RBC due to non-availability of Hb content in cellular medium [29]. The damaging of toxicant on erythrocyte may be secondary, as resulting from a primary action of toxicant on erythropoietic tissues on which there exists a failure in red cell production and or due to increase in the erythrocyte destruction [30]. Moreover, Adhikari [31] also reported that the RBC count and Hb concentration decrease may depend on, sex as observed in this study.…”

Section: Discussionmentioning

confidence: 99%

Influence of Sex on Haematological Response of Clarias gariepinus Juveniles Treated with Atrazine and Metalochlor

Adi¹,

Akinrotimi²

2017

Trends in Green chem

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Section: Discussionmentioning

confidence: 99%

Influence of Sex on Haematological Response of Clarias gariepinus Juveniles Treated with Atrazine and Metalochlor

Adi¹,

Akinrotimi²

2017

Trends in Green chem

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“…Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) were calculated using the formulae mentioned by (Dacie and Lewis, 1991). Total white blood cells (WBC) were counted using an improved Neubauer hemocytometer (Mgbenka and Oluah, 2003;Shah and Altindg, 2005). Blood were diluted 1:20 with a diluting fluid and placed in hemocytometer.…”

Section: Blood Analysismentioning

confidence: 99%

Hematological and biochemical characters of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) cultivated using methyltestosterone

Sayed

Moneeb

2015

The Journal of Basic & Applied Zoology

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The use of steroid-treated feeds as 17 a-methyltestosterone for the production of all-male populations is widespread in tilapia aquaculture. The aim of the present study was to investigate the misuse effects of methyltestosterone on monosex farmed Nile tilapia, Oreochromis niloticus by evaluation of hematological and biochemical values. The fishes were obtained from four localities (Assiut as a control, Beheira, Alexandria and Kafr el-Sheikh: three farms from each governorate as farmed monosex produced using methyltestosterone). The total erythrocyte count, hemoglobin rate, hematocrit percentage, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), white blood cell count, blood platelets, lymphocyte, monocyte, neutrophils and basophils were determined. The results showed that there were changes in the erythrocytic series and in the defense white blood cells. Activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), glucose, cholesterol, total protein, uric acid and creatinine were determined for biochemical study. These alterations are considered an indication for performance and health of fish in the monosex culture medium indicating the side effects of overdose induction of MT. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http:// creativecommons.org/licenses/by-nc-nd/4.0/).

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“…Hb concentration was measured with Hb test kit using the cyanmethemoglobin method (Larsen and Snieszko, 1961). RBC and WBC counts were counted under light microscope with an improved Neubauer haemocytometer (Mgbenka and Oluah, 2003;Altindg, 2004, 2005). The derived hema-tological indices of MCV, MCH and MCHC were calculated using standard formulae as described by Jain (1986) MCV will be calculated in femtoliters = PCV/RBC x 10; MCH was calculated in picograms = Hb/RBC x 10; and MCHC = (Hb in 100 mg blood / Hct) x 100.…”

Section: Hematological Studiesmentioning

confidence: 99%

Impact of nickel (Ni) on hematological parameters and behavioral changes in Cyprinus carpio (common carp)

Al‐Ghanim¹

2011

Afr. J. Biotechnol.

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The effect of nickel on hematological parameters and behaviour in Cyprinus carpio after a 96 h exposure to nickel test was investigated. Cyprinus carpio fingerlings were obtained from local fish hatchery. Morphometric characteristics of experimental fish were recorded. Fish of both sexes were stocked without discrimination. The fish were exposed to different concentrations (0, 6, 9, 12, 15 and 18 mg/l) of nickel sulphate using standard screening procedure. The mortality rate of the experimental fish was increased with increase in concentration of nickel. The 96 h median lethal concentration (96 h LC50) was 12.44 mg Ni/L using the logarithmic method with dose-mortality regression line y = 188.224x -86.52. The dissolved oxygen concentration decreased with increase in the level of Ni. All the blood parameters (erythrocyte, leucocytes, hematocrit and hemoglobin count) decreased with increasing dose of nickel and become significantly lower (P < 0.05) at higher concentration when compared with the control. Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were also lowered with concentration of toxicant when compared with the control. The results of the present study showed that a short-term exposures to high levels of nickel induced stress reactions in C. carpio. Some adaptive changes were observed; preparing the organism to an increased energy expense, whereas other changes showed a considerable immunosuppressive effect of stress. It was concluded that changes observed indicate that hematological parameters can be used as an indicator of Ni stress in C. carpio.

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Effect of Gammalin 20 (Lindane) on Differential White Blood Cell Counts of the African Catfish, Clarias albopunctatus

Cited by 25 publications

References 0 publications

Influence of Sex on Haematological Response of Clarias gariepinus Juveniles Treated with Atrazine and Metalochlor

Influence of Sex on Haematological Response of Clarias gariepinus Juveniles Treated with Atrazine and Metalochlor

Hematological and biochemical characters of monosex tilapia (Oreochromis niloticus, Linnaeus, 1758) cultivated using methyltestosterone

Impact of nickel (Ni) on hematological parameters and behavioral changes in Cyprinus carpio (common carp)

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