Effect of garlic-derived allyl sulphides on morphogenesis and hydrolytic enzyme secretion inCandida albicans

Abstract

The effect of diallyl sulphide (DAS) and diallyl disulphide (DADS) on secretion of hydrolytic enzymes and dimorphism has been investigated in two strains of Candida albicans. Minimum inhibitory concentration (MIC) of DADS and DAS was determined to be 500 μg/ml and 40 μg/ml, respectively for a clinical isolate (accession #3043) and 450 μg/ml and 50 μg/ml, respectively, for a reference strain (ATCC 90028). At one-half of the minimum inhibitory concentration (MIC), DAS and DADS inhibited proteinase secretion by 24% and 35%, respectively, in the clinical strain, and 28% and 44%, respectively, in the reference strain. Inhibition of phospholipase secretion at one-half MIC of DAS and DADS was 27% and 60%, respectively, in the clinical strain and 31% and 64%, respectively, for the reference strain. Hyphal induction at 300 min in the reference strain was 15% (at one-half MIC of DAS) and 5% (at one-half the MIC of DADS) as compared to control (90% hyphal formation). Hyphal induction in the clinical strain was 16% (one-half the MIC of DAS) and 8% (one-half the MIC of DADS) compared to 95% in the control. To conclude, both DAS and DADS significantly inhibit proteinase, phospholipase secretion and dimorphism in C. albicans. These compounds can therefore be explored for their therapeutic potential against C. albicans.

Biosynthesis, Characterization, and Antifungal Activity of the Silver Nanoparticles Against Pathogenic Candida species

“…Proteinase assay was performed according to Yousuf et al [35]. Briefly, Candida strains were exposed to different concentrations of silver nanoparticles (MIC/8, MIC/4, MIC/2, and MIC) for 5 min.…”

Species Diversity, Antifungal Susceptibility, and Virulence Attributes of Candida Colonising the Oral Cavities of Adult Diabetic Patients

“…Candida parapsilosis ATCC 22019 was used as a negative control. Proteinase activity was determined by calculating the zone of digestion ( ) using the formula-ratio of the diameter of the colony to the sum of diameter of the colony and the zone (in mm) [13,14].…”

Assessment of potential use of garlic (Allium sativum) against growth of microbes

“…For example, electron microscopic investigations revealed that addition of garlic can damage the cell membrane of Candida albicans and thereby inhibit the multiplication of this fungus (Lemar et al, 2002). Strong evidence in support of a reduction in the growth of Candida with garlic application was also provided by (Yousuf et al, 2011). Apart from Candida, many researchers have analyzed the effect of garlic on other fungi.…”