Effect of gelatin–ionic liquid functional polymers on glucose oxidase and horseradish peroxidase kinetics

Lourenço, Nuno M. T.; Österreicher, Johannes A.; Vidinha, Pedro; Barreiros, Susana; Afonso, Carlos A. M.; Fonseca, Luís P.

doi:10.1016/j.reactfunctpolym.2011.01.006

Cited by 31 publications

(12 citation statements)

References 20 publications

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“…Recently, Ozokur et al, fabricated a glassy carbon electrode modified with manganese oxide decorated with platinum nanoparticles and monitored bio-catalytic activity of pyranose oxidase bridged by ILs unit [19]. Lourenco et al 2011 studied the effect of different ILs 1-ethyl-3-methyl-imidazolium ethyl sulphate, 1-butyl-3-methyl-imidazolium dycianamide and 1-butyl-3-methyl-imidazolium chloride, 1-butyl-3-methylimidazolium tetrafluoroborate, gelatin and water on the activity of GOx and horse redish peroxidise [20]. Thus, there is wide scope for development of electrochemical biosensor using biopolymer and ILs for glucose sensor.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical response of agar ionogels towards glucose detection

et al. 2015

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We have reported a sensing platform comprising of agar ionogel (IGs) made in ionic liquid solutions (1-octyl-3-methyl imidazolium chloride [C8mim][Cl] and 1-ethyl-3-methyl imidazolium chloride [C2mim][Cl]) and used it for glucose oxidase (GOx) immobilization for glucose detection. The ionogels were deposited onto indium tin oxide (ITO) coated glass plate using the drop casting technique. These Agar-[C8mim][Cl]/ITO (Ag-C8/ITO) and Agar-[C2mim][Cl]/ITO (Ag-C2/ITO) substrates were used for immobilization of GOx, which was selected as a model enzyme to investigate its interaction with these electrodes using electrochemical techniques. Structural and morphological studies of these GOx/Ag-C8/ITO and GOx/Ag-C2/ITO electrodes were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and electrochemical techniques (CV). These GOx/Ag-C8/ITO and GOx/Ag-C2/ITO bioelectrodes exhibited improved glucose detection capability in the concentration range of 0.27-16.7 mM and 0.28-5.6 mM with sensitivity ≈ 4.1µA mM -1 /cm -2 and 14.6 µA mM -1 cm -2 , respectively. The value of apparent Michaelis-Menten constant (K m ) was 0.023 mM and 0.0007 mM for the aforesaid two cases respectively. It is shown that, customization of agar hydrogels in green solvent medium widens the scope of its potential applications as glucose sensing in real samples. IntroductionRecently, ionic liquids (ILs) have received much attention as materials with enormous practical applications because of their unique properties that include exceptional ionic conductivity, biocompatibility, electrochemical, thermal stability and non-flammability making them particularly attractive in the development of electrochemical biodevices with long-term stability.As variability in their application potential arises from the availability of a range of ILs molecules with various combinations of anions, cations, hydrophilic head groups, and hydrophobic chain lengths [1-6]. It has been adequately realized that ILs are the green solvents of the future. The novel and remarkable attributes of ILs can be realized from the following facts:(i) ILs can dissolve cellulose [7], (ii) maintain biological activity of DNA in solutions over an extended period [8] and (iii) inhibit pH dependent aggregation of proteins [9]. Moreover ionic liquids are room-temperature molten salts that are increasingly used in electrochemical devices, such as in batteries, fuel cells, and biosensors [10]. The ILs has been used for the different biosensing application because ILs increases the stability of biomolecules and also improves the sensitivity and response time of biosensor [11-12]. ILs is being used for development of different electrochemical biosensor for clinical diagnosis including glucose detection. Liu et al. have reported hydrolysis of tetraethyl orthosilicate in 1-butyl-3-methylimidazolium tetra-fluoroborate (BMIMBF4) based hydrogen peroxide electrochemical biosensor [13]. They have reported that in BMIMBF4, IL improved the film quality without forming a...

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Section: Introductionmentioning

confidence: 99%

Electrochemical response of agar ionogels towards glucose detection

et al. 2015

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“…Gelatin was prepared according the protocol determined by Lourenço et al [ 20 ]. Four-hundred milligrams of powdered gelatin were dissolved in 1 mL of distilled water (60 °C) by stirring for 20 min; then, 2 mL of PBS 7.4 (60 °C) was added and mixture was stirred for another 30 min.…”

Section: Methodsmentioning

confidence: 99%

Color Change of Phenol Red by Integrated Smart Phone Camera as a Tool for the Determination of Neurotoxic Compounds

Kostelník

Cegan

Pohanka

2016

Sensors

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The use of a cell phone as a detection system is easy, simple and does not require trained personnel, which is in contrast to standard laboratory instruments. This paper deals with immobilization of acetylcholinesterase (AChE) in a gelatin matrix, and phenol red, as an indicator of AChE activity, is used in order to establish a method that is easily compatible with a camera device. AChE splits acetylcholine into choline and acetic acid, which changes the pH of a medium, resulting in a phenol red color change. The coloration changed in presence of an AChE inhibitor. Measurements were performed on 3D-printed, tube-shaped holder, and digital photography, with subsequent analysis of red-green-blue (RGB), served for assay purposes. Calibration of AChE inhibitors, tacrine and galantamine, was performed, with limit of detection equal to 1.1 nM and 1.28 µM, respectively. Interferences were also measured, resulting in a proof-of-method stability. The method was further successfully validated for the standard Ellman’s assay, and verified on murine plasma samples spiked with inhibitors.

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“…Moreover, these electrolytes may often be used as printable "inks" [31,94]. Several authors have been using these IGs for the development of biosensing devices, namely for the immobilization of oxidoreductases, such as glucose oxidase (GOD) and horseradish peroxidase (HRP) [93,95,96]. Lourenço et al prepared glucose paper test strips by the physical deposition of gelatin-1-ethyl-3-methyl-imidazolium ethyl sulfate ((Emim)EtSO4) containing GOD and HRP, as well as color-generating precursors [95].…”

Section: Gelatinmentioning

confidence: 99%

Marine-Derived Polymers in Ionic Liquids: Architectures Development and Biomedical Applications

et al. 2020

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Marine resources have considerable potential to develop high-value materials for applications in different fields, namely pharmaceutical, environmental, and biomedical. Despite that, the lack of solubility of marine-derived polymers in water and common organic solvents could restrict their applications. In the last years, ionic liquids (ILs) have emerged as platforms able to overcome those drawbacks, opening many routes to enlarge the use of marine-derived polymers as biomaterials, among other applications. From this perspective, ILs can be used as an efficient extraction media for polysaccharides from marine microalgae and wastes (e.g., crab shells, squid, and skeletons) or as solvents to process them in different shapes, such as films, hydrogels, nano/microparticles, and scaffolds. The resulting architectures can be applied in wound repair, bone regeneration, or gene and drug delivery systems. This review is focused on the recent research on the applications of ILs as processing platforms of biomaterials derived from marine polymers.

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Effect of gelatin–ionic liquid functional polymers on glucose oxidase and horseradish peroxidase kinetics

Cited by 31 publications

References 20 publications

Electrochemical response of agar ionogels towards glucose detection

Electrochemical response of agar ionogels towards glucose detection

Color Change of Phenol Red by Integrated Smart Phone Camera as a Tool for the Determination of Neurotoxic Compounds

Marine-Derived Polymers in Ionic Liquids: Architectures Development and Biomedical Applications

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