The soybean crop (Glycine max) is known for its high oil and protein content, making it a valuable resource for animal feed and a crucial ingredient in vegan and vegetarian food products. Soybean is a thermophilic short-day plant, demanding specific climatic conditions for successful cultivation. In an effort to expand soybean cultivation to northern regions, a variety of trials were conducted. The aim of this study was to determine the most suitable soybean varieties for cultivation in Northern Poland. The field trials were conducted in nine locations, in the years 2020–2022. Yield, fat content, and protein content were the observed characteristics. Results for 13 varieties had been collected and were analysed using the AMMI model. The genotype–environment interaction provides information that supports estimations of the stability of certain varieties. AMMI-adjusted means, WTOP3, WAAS and GSI indices were calculated in order to assess the suitability of those varieties for cultivation in Northern Poland. It was shown that the Amiata variety had the highest mean yield among the tested varieties, whilst the Erica variety was the most stable. The Abelina variety had the lowest value of the GSI index. For fat content, the Ambella variety had the highest mean and the lowest values of the GSI index, whereas the ES Comandor variety was the most stable. For protein content, the Nessie PZO variety had the highest mean, the Aurelina variety was the most stable and had the lowest values of the GSI index. Thus, the Abelina, Ambella, and Aurelina varieties are the most favourable varieties for cultivation in that region.