Effect of genotype and growing season on nitrate accumulation and expression patterns of nitrate transporter genes in potato (<i>Solanum tuberosum</i>L.)

Mahmoud, Mahmoud A.; Abid, Ghassen; Chikh-Rouhou, H.; Razgallah, Nesrine; Hassen, Abdennaceur

doi:10.1080/03650340.2016.1154544

Cited by 16 publications

(5 citation statements)

References 35 publications

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“…The availability of nutrients is a crucial factor in root development, and nitrate ions regulate the growth and root architecture of higher plants (M'hamdi et al, 2016; Nawaz, Chen, et al, 2018; Nawaz, Han, et al, 2018; Stitt, 1999). External nitrate induces dual effects on root growth both at doze, a stimulatory effect at a low level, and a systemic inhibitory effect at high nitrate concentration.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the inhibitory nature of high nitrate during adventitious roots formation in the apple rootstock

Zhang

Tahir

et al. 2021

Physiologia Plantarum

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Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the inhibitory nature of high nitrate during adventitious roots formation in the apple rootstock

Zhang

Tahir

et al. 2021

Physiologia Plantarum

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“…NO 3 − and NH 4 + are two sources of nitrogen absorbed by plant roots from soil [41]. Mhamdi et al reported that the expression levels of StNRT1.2, StNRT1.5, and StNRT2.1 were higher in N-efficient varieties [42], suggesting NRT plays a positive and important role.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of CBL-Interacting Protein Kinases 23 Improves Tolerance to Low-Nitrogen Stress in Potato Plants

Huang,

Lu,

et al. 2024

Horticulturae

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CBL-interacting protein kinases (CIPKs) play important regulatory roles in plant growth development and abiotic stress tolerance. However, the biological roles of these genes in response to low-nitrate (LN) stress in potato plants have not been determined. Here, we reported that StCIPK23 was expressed mainly in roots and leaves. StCIPK23 was located mainly in the cell membrane, nucleus, and cytoplasm. Further research suggested that, compared with wild-type (WT) plants, StCIPK23-overexpressing plants were taller and had significantly greater nitrate and ammonium nitrogen contents under LN stress. StCIPK23 overexpression can increase StAT, StNRT2.1, StNR, StGS1-3, and StGOGAT expression levels in StCIPK23 transgenic seedlings compared to those in WT plants under LN stress. The results of yeast two-hybrid and luciferase complementation imaging experiments suggested that StCIPK23 could interact with StCBL3. Real-time reverse transcription–PCR revealed the StCIPK23 expression level peaked at 6 h and the StCBL3 expression level peaked at 9 h in the roots under LN stress. In conclusion, we found that StCIPK23 and StCBL3 form a complex to regulate the expression of key genes in the nitrogen metabolism pathway to improve LN tolerance in potato plants.

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“…Additionally, it was observed that the expression of AtNRT2.1 increased transiently during short time starvation and decreased to the level slightly higher than before the treatment during prolonged N starvation (Lezhneva et al, 2014). In potato, StNRT2.1 was shown to be higher expressed in the cultivars accumulating more nitrate in the tubers, suggesting that also in potato NRT2.1 is a key factor for nitrate uptake (M'hamdi, Abid, Chikh-Rouhou, Razgallah, & Hassen, 2016). An increase in the StNRT2.1 abundance under N deficiency may likely be linked to a decrease in the tissue nitrate concentration in 'Topas', inducing the transcriptional upregulation of this high affinity transport system.…”

Section: Pm Proteins Involved In the Tolerance Of Potato To N Deficiencymentioning

confidence: 95%

Plasma membrane intrinsic proteins PIP1;1 and PIP1;3 contribute to the tolerance to nitrogen deficiency in potato

Józefowicz

Bienert

Garibay

et al. 2020

Preprint

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Nitrogen (N) deficiency is one of the major constraints for potato (Solanum tuberosum L.) production, due to its shallow root system and poor capabilities of N mobilization from the deeper layers of the soil. The aim of this study was to elucidate plasma membrane (PM) proteins potentially involved in the tolerance towards N deficiency. We compared the PM proteome of the two potato cultivars 'Lambada' and 'Topas', contrasting in their N deficiency response under in vitro conditions. Using mass spectrometry we identified 65 proteins, which were differentially abundant in the two cultivars when submitted to N deficiency. Amongst those, candidate proteins were selected based on their potential to be involved in N transport under deficient conditions. Two members of the aquaporin family, StPIP1;1 and StPIP1;3, were studied in more detail. By means of a yeast growth assay we showed that these proteins do not facilitate transport of ammonia. The Arabidopsis (Arabidopsis thaliana) knockout mutants AtPIP1;1 and AtPIP1;3 showed a reduced N content and accumulation of anthocyanins under N deficiency. We conclude that PIP1;1 and PIP1;3 are indirectly involved in the N uptake under N deficient conditions and contribute to an increased N deficiency tolerance. Summary statement Root plasma membrane proteome profiling of two potato cultivars reveals the participation of the two members of the aquaporin family PIP1;1 and PIP1;3 in the cultivar specific response to nitrogen deficiency.

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Effect of genotype and growing season on nitrate accumulation and expression patterns of nitrate transporter genes in potato (Solanum tuberosumL.)

Cited by 16 publications

References 35 publications

Transcriptome analysis reveals the inhibitory nature of high nitrate during adventitious roots formation in the apple rootstock

Transcriptome analysis reveals the inhibitory nature of high nitrate during adventitious roots formation in the apple rootstock

Overexpression of CBL-Interacting Protein Kinases 23 Improves Tolerance to Low-Nitrogen Stress in Potato Plants

Plasma membrane intrinsic proteins PIP1;1 and PIP1;3 contribute to the tolerance to nitrogen deficiency in potato

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