Effect of genotype and medium culture content on microspore-derived embryo formation in Chinese cabbage (Brassica rapa ssp. chinensis) cv. Lastochka

Abstract

The influence of different factors on microspore embryogenesis in Chinese cabbage (B. rapa ssp. chinensis) was studied. A genotype dependence for embryo formation was observed. The majority of embryos and plants were obtained from microspores isolated from flower buds (2-2.9 mm in length) and cultured in the NLN liquid medium with 13% sucrose (w/v) supplemented with 24 epibrassinolide and 1% activated charcoal. Embryos cultured on the 1/2 Murashige Skoog culture medium with 2% sucrose (w/v), 0.1 mg/L benzylaminopurine, and 3 g/L Phytagel stimulated the formation of secondary embryos that resulted in development of large number of doubled haploid plants.

Proteomic variations after short-term heat shock treatment reveal differentially expressed proteins involved in early microspore embryogenesis in cabbage (Brassica oleracea)

“…The chromosomes of the haploids can be doubled by colchicine treatment or subcluture, and the double haploids (DHs) are genetically homozygous and can be used as breeding lines directly. To date, more than hundreds of plant species have been reported and are especially widely used in Brassica crop breeding programs (Telmer, Newcomb & Simmonds, 1995;Shmykova, Shumilina & Suprunova, 2016;Shumilina, Shmykova & Bondareva, 2015). In recent years, many researchers have studied isolated microspore culture technology in Brassica and strongly improved the microspore embryogenesis rate (Yuan et al, 2011;Shumilina, Shmykova & Bondareva, 2015).…”

“…To date, more than hundreds of plant species have been reported and are especially widely used in Brassica crop breeding programs (Telmer, Newcomb & Simmonds, 1995;Shmykova, Shumilina & Suprunova, 2016;Shumilina, Shmykova & Bondareva, 2015). In recent years, many researchers have studied isolated microspore culture technology in Brassica and strongly improved the microspore embryogenesis rate (Yuan et al, 2011;Shumilina, Shmykova & Bondareva, 2015). In particular, extensive studies have shown that pretreatment, usually performed under heat or cold treatment, is essential for Brassica embryo induction (Joosen et al, 2007;Yuan et al, 2011;Yuan et al, 2012).…”

Proteomics variations after short-term heat shock treatment reveal differentially expressed proteins involved in early microspore embryogenesis in cabbage (Brassica oleracea)

“…Microspore embryogenesis has been described for more than 250 plant species, and efficient protocols are available for Brassica species [8]. In recent years, many researchers have studied and improved isolated microspore culture technology from genotypes, the growth environment of donor plants, microspore development stages, pretreatment conditions, plant regeneration, ploidy identification and chromosome doubling, which has improved the microspore germination rate of some of the genotypes compared to their original state [9,10]. Pretreatment is essential to embryo production, microspore and pollen isolated from cruciferous plants can produce haploid embryos in vitro without the need for intermediate callus stage under appropriate stress treatment.…”

GENERATION OF Rubus arcticus L. HAPLOIDS THROUGH in vitro MICROSPORE CULTURE TECHNIQUE

“…В исследованиях для индукции эмбриогенеза и получения вторичных эмбриоидов активно используются разные концентрации 6-БАП в качестве регулятора роста (25). В нашей работе 6-БАП индуцировал прямой эмбриоидогенез.…”