Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)

Abstract

Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 µM 2,4-D and 1 µM Kinetin or 5 µM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 µM 2,4,5-T or the combination of 10 µM 2,4-D and 1 µM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry. To cite this article: Z.

Somatic embryogenesis and in vitro plant regeneration in moth bean [Vigna aconitifolia (Jacq.) Marechal]: a recalcitrant grain legume

“…Factors such as the effects of PGRs, reduced nitrogen, plant species and genotype of the cultured material have an effect on the induction and proliferation of embryos (El Abidine Triqui et al 2008). Even radiation can play a key role in somatic embryogenesis (Begum et al 2008).…”

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

“…These results were similar to those of Belarmino and Gonzales (2008) who revealed that picloram is essential for callus induction and somatic embryogenesis in yam. Picloram has been successfully employed for the induction of friable embryogenic calli in root and tuber crops, especially cassava, sweet potato, and yam (Nyaboga et al 2013;Triqui et al 2008;Belarmino and Gonzales 2008). The copper sulfate used in the media combination with auxins creates oxidative stress conditions that promote both cell division and embryogenic cell formation, as reported by Pasternak et al (2002).…”

In silico identification and characterization of conserved miRNAs and their target genes in sweet potato (Ipomoea batatasL.) Expressed Sequence Tags (ESTs)

“…Its stems and foliage are also used as forage. 27 Sweet potato is also rich in vitamin A, B, and C, iron, phosphorous, and calcium. In recent years, several methods have been employed in the improvement of sweet potato crop but breeding is constrained by the complexity of the genetics of this out-crossing hexaploid crop (2n = 6x = 96) and lack of genomic resources.…”