Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata

Bergmann, Ben A.; Stomp, Anne-Marie

doi:10.1007/bf00035970

Cited by 9 publications

(9 citation statements)

References 17 publications

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“…In vitro culture response is mediated mainly by genotype (Bergmann and Stomp, 1994) and explant physiological state (Bonga, 1981). There are studies that show that different genotypes of the same species respond differently to the culture medium (Pinus contorta, von Arnold and Eriksson, 1981; P. populus, P. radiata and Picea abies, Bergmann and Stomp, 1994;Pinus caribaea, Go et al, 1993;mango, Litz et al, 1998). To evaluate such an effect using the best conditions for adventitious bud induction (SH medium 23 mM K cultured for 30 d and later subcultured to SH 50 medium) found in our previous experiments, we worked with immature seeds from four localities.…”

Section: Resultsmentioning

confidence: 96%

“…In Pinus banksiana the highest root production was obtained by culturing the shoots on medium with NAA (40±75%), whereas with IBA root production was null or very low (,5%) . In other conifer species rooting has been achieved with hormone mixtures (Bergmann and Stomp, 1994) or mixtures of substrates on liquid medium and with auxins (Harry et al, 1995).…”

Section: Resultsmentioning

confidence: 99%

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In vitro regeneration of plantlets from immature zygotic embryos of Picea chihuahuana Martínez, an endemic mexican endangered species

Mata-Rosas

Manuel

Avila

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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Adventitious buds were induced from immature embryos of Picea chihuahuana, a species from the north of Mexico. The medium was Schenk and Hildebrandt supplemented with benzyladenine and kinetin, alone or in combination with naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid. Adventitious buds were obtained through a wide range of growth regulator concentrations, mainly from the cotyledonary region. Kinetin was more effective than benzyladenine in shoot induction. The optimum shoot induction medium contained 23.0 mM kinetin, on which 11.2 adventitious buds per embryo were obtained. Shoot development and elongation were achieved on basal SH medium at 50% concentration (SH 50) without growth regulators and improved with the reduction of sucrose concentration to 10 g l 21 . Differential response appeared to be associated with the provenance of the seeds, relative to the percentage of explants that responded as well as the number of adventitious buds produced per embryo. A low percentage of shoot rooting was obtained with a 24-h pulse on a medium with 16.1 or 26.85 mM of naphthaleneacetic acid. In the process of micropropagation of Picea chihuahuana, rooting is still the limiting factor.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

In vitro regeneration of plantlets from immature zygotic embryos of Picea chihuahuana Martínez, an endemic mexican endangered species

Mata-Rosas

Manuel

Avila

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…Clones 12 and 40 exhibited higher rooting percentages as well as number. Role of Kalia et al·Silvae Genetica (2004) 53-5/6, 212-220 genotypic variations in rooting has been encountered in studies of different research groups (HORGAN and HOLLAND, 1989;BERGMANN and STOMP, 1994;SCALTSOYIANNES et al, 1994). They recorded variation in percentage of shoots rooted and number of roots per shoot.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Clonal Variability in Shoot Coppicing Ability and in vitro Responses of Dalbergia sissoo Roxb

Kalia

Sharma

2004

Silvae Genetica

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SummaryClonal variations were observed amongst 12 clones of Dalbergia sissoo belonging to four states (U.P, Uttaranchal, Haryana and Rajasthan) of India, representing four different geographical zones in respect of ex vitro shoot coppicing ability and in vitro responses. Coppicing ability of shoot hedges of clones exhibited significant variation which ranged from average of 13.81 coppiced shoots (Clone 40, Uttar Pradesh) to 9.29 (Clone 64, Haryana). Comparative analysis of clones from different regions in respect to their coppicing ability revealed that clones from U.P had higher coppicing ability whereas those from Haryana proved to be least coppicers. Regional variations were also exhibited in the in vitro multiple bud induction ability on nodal explants excised from shoot hedges of clones (mean number of buds induced and percentage of cultures forming multiple buds). Regional as well as inter clonal variations were recorded in the shoot proliferation efficiency as well as rootability of microshoots of these clones as well as their optimal plant growth regulator requirements. BAP alone (2.5 µM) was sufficient for inducing multiple buds on cultured nodal explants of Uttaranchal and Uttar Pradesh region clones. On the contrary, clones from Rajasthan and Haryana had higher optimal requirement of BAP and in addition, they required media to be supplemented with auxin (NAA) for induction of multiple buds on explants. Correlation analysis between shoot coppicing ability of clones and in vitro performances of explants of these clones cultured on 2.5 µM BAP indicates a positive correlation. Observation lays credence to our view that these characters are genetically controlled and shoot coppicing can be used as a marker character in optimizing in vitro performance of clones. Using the information generated by this paper in vitro production of elite planting material can be maximized by ameliorating plant growth regulator requirement in the medium.

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“…Ainsi chez A. raddiana il a notamment été remarqué que leurs capacités à l'enracinement baissaient de près de 88 % dès la deuxième subculture sur milieux de multiplication en présence de concentrations faibles ou moyennes (0,1 à 1,2 mg L -1 ) d'ANA ou d'AIB [7]. La nécessité d'utiliser des doses d'auxine plus élevées (6 à 8 mg L -1 d'ANA) et surtout celle de dissocier les étapes de l'induction et de l'expression des racines a déjà été mise en évidence chez Pinus radiata [2], comme chez A. senegal [1]. Chez cette espèce, un traitement inductif de 12 jours à l'ANA à 8 mg L -1 apparaît meilleur qu'un traitement de 6 jours et permet une nette améliora-tion du taux d'enracinement des microboutures dès la deuxième subculture.…”

Section: Discussionunclassified

“…raddiana une application permanente d'AIB ou d'acide naphtalène acétique (ANA) a permis d'observer des fréquences de 20 % à 65 % d'enracinement [11]. L'application d'hormones est indispensable et des auxines comme l'AIB ou l'ANA appliquées de manière transitoire permettraient d'améliorer l'enracinement d'espèces forestières cultivées in vitro [2,16].…”

Section: Introductionunclassified

Induction in vitro de l'enracinement de microboutures d'Acacia tortilis subsp. raddiana par traitement transitoire � l'auxine

Sané¹,

Borgel²,

Chevallier³

et al. 2001

Ann. For. Sci.

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Les mesures effectuées après 28 jours de culture ont montré que la nature de l'auxine utilisée pour induire la rhizogenèse a une influence significative aussi bien sur la fréquence de l'enracinement que sur la morphologie du système racinaire qui se met en place. Avec l'ANA les racines sont peu nombreuses (1 à 5 racines par bouture) et robustes et orthotropes (4,5 à 5 cm en moyenne) dans 80 % des cas et avec l'AIB, elles sont très nombreuses (10 à 15 racines par bouture) et fines et plagiotropes (3 à 3,7 cm) dans 68 % des cas. Acacia tortilis raddiana / microbouture / auxine / induction / rhizogenèseAbstract -Transient auxin treatment for in vitro rooting of microcuttings of Acacia tortilis subsp. raddiana. An auxin in vitro pretreatment (IBA or NAA at 10 to 20 mg L -1 ) combined with a low concentration of Kinetin (0.01 mg L -1 ) during 10 or 20 days allowed to improve the rooting (number and root length) of Acacia tortilis raddiana microcuttings after transfer on Lloyd and Mc Cown medium (WPM) without hormone. After microcuttings transfer in expression medium, 56% are rooted and the rooting frequency can reach 80% after pretreatment with IBA at 10 mg L -1 . Measurements made after 28 days show that the auxin type used for inducing rhizogenesis has a significant effect, both on rooting percentage and rooting system morphology. For 80% of plantlets, the number of roots is low with NAA (1 to 5) but roots are strong and orthotropic (4.5 to 5 cm on average) when with IBA roots are numerous (10 to 15), but thin and plagiotropic (3 to 3.7 cm) for 68% of rooted plantlets. Acacia tortilis raddiana / microcutting / auxin / induction / rhizogenesisAnn. For. Sci. 58 (2001) [431][432][433][434][435][436][437] 431

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Effect of genotype on rooting of hypocotyls and in vitro-produced shoots of Pinus radiata

Cited by 9 publications

References 17 publications

In vitro regeneration of plantlets from immature zygotic embryos of Picea chihuahuana Martínez, an endemic mexican endangered species

In vitro regeneration of plantlets from immature zygotic embryos of Picea chihuahuana Martínez, an endemic mexican endangered species

Evaluation of Clonal Variability in Shoot Coppicing Ability and in vitro Responses of Dalbergia sissoo Roxb

Induction in vitro de l'enracinement de microboutures d'Acacia tortilis subsp. raddiana par traitement transitoire � l'auxine

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