An experiment was conducted to evaluate the biocontrol potential of arbuscular mycorrhizal fungus, Scutellospora sp. against root knot nematode, Meloidogyne incognita on tomato under glasshouse conditions. Different dosages of Scutellospora sp. @ 5g, 10g, 15g, 20g and 25g/plant were tested against M. incognita and compared with carbofuran 3G and an untreated control. Among the various dosages evaluated, application of Scutellospora sp. @ 25g/plant was found effective, which enhanced the plant growth parameters to the tune of 58.7% of shoot height, 57.5% of shoot weight, 58.5% of root length, 63.8% of root weight, 59.3% of fruit weight, 63.6% of AM colonization, spore load (167.3/100cc soil) and 0.54% of total phosphorus content and 56.3 and 60.5% reduction in soil and root population of M.incognita over untreated control.