Effect of Glucose Metabolism Related Protein-1 on Proliferation and Apoptosis of MIN6 Cells

Abstract

<jats:p>Apoptosis of islet <jats:italic>β</jats:italic> cells participates in type 1 diabetes. Exendin-4 promotes islet <jats:italic>β</jats:italic> cell proliferation and inhibits apoptosis. Glucose metabolism related protein-1 (GMRP-1) has the similar effects on islet <jats:italic>β</jats:italic> cell. Whether the effect of exendin-4 islet <jats:italic>β</jats:italic>-cells is regulated by GMRP-1 is not clear. GMRP-1 siRNA or pEGFP-C3-GMRP-1 recombinant plasmid was transfected into MIN6 cells and treated with t-BHP in the presence of exendin-4 (0, 50, 100 and 200 nmol/L) followed by analysis of cell proliferation ability by CCK8 assay, apoptosis by flow cytometry, as well as GMRP-1 protein level by Western blot. After transfection of the plasmid with high expression of GMRP-1, the number of MIN6 cells was increased significantly, and the apoptosis rate was reduced <jats:italic>(P &lt;</jats:italic> 0.05). With increased exendin-4 concentration, the number of cell proliferation was increased gradually with reduced cell apoptosis <jats:italic>(P &lt;</jats:italic> 0.05). After the expression of GMRP-1 was decreased after GMRP-1 siRNA interference fragment transfection, the number of MIN6 cells was decreased significantly and the apoptosis was elevated (P &lt; 0.05), whereas, exendin-4’s effect on the proliferation of MIN6 cells and inhibition of apoptosis was attenuated. GMRP-1 affects the proliferation and apoptosis of MIN6 cells, and regulates exonin-4-induced MIN6 cell proliferation and apoptosis.</jats:p>