Effect of glucose on the superoxide dismutase production in fungal strain<i>Humicola lutea</i>

Angelova, Maria; Genova, Lubka K.; Slokoska, L; Пашова, Светлана

doi:10.1139/m95-136

Cited by 19 publications

(9 citation statements)

References 19 publications

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“…This is in contrast with our previous report [ 8 ], which suggest that SOD synthesis in the fungal strain H. lutea 110 is not a subject to catabolite repression. But changing the glucose concentration from low to high in presence of Cu ions caused significant decrease in SOD activity in H. lutea 103.…”

Section: Fig 3 Deinduction Of Sod Synthesis By Removal Of Cu Ionscontrasting

confidence: 99%

“…As it is known, in many cases catabolite repression in microorganisms is correlated with lowered levels of cAMP [ 8,12,13 ]. Our present results (Table 1) illustrate the reversal effect of cAMP on SOD synthesis by H. lutea cells in the presence of glucose.…”

Section: Fig 3 Deinduction Of Sod Synthesis By Removal Of Cu Ionsmentioning

confidence: 99%

“…On the other hand, catabolite repression is a mechanism preventing transcriptional expression of genes required for the degradation of the less-preferred substrate in the presence of the preferred substrate [ 5,6 ]. Until now, very few studies have been carried out on the role of these mechanisms in regulation of SOD synthesis [7][8][9]. Furthermore, there are no investigations on the relationship between heavy metal stress and regulation of SOD synthesis.…”

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confidence: 99%

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Regulation of Superoxide Dismutase Synthesis in Humcola lutea Cells under Cu2+ Stress Conditions

Krumova¹,

Abrashev²,

Miteva-Staleva³

et al. 2013

Proceeding BAS

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The superoxide dismutase (SOD) is the first enzyme to respond against oxygen radicals and to offer the greatest response to oxidative stress. The great interest in SODs is a result of their important physiological role and therapeutic potential. The fungal strain Humicola lutea 103 has been selected as an effective producer of Cu/Zn-SOD exhibiting protective effect against influenza virus infection and myeloid Graffi tumour. The present paper studies the role of control mechanisms induction and catabolite repression on SOD synthesis under copper stress conditions. Cu ions cause rapid and profound induction of enzyme activity by dose-and time-dependent manner. The deinduction experiments also demonstrate the induction model of SOD synthesis. The results suggest that SOD synthesis in the fungal strain H. lutea 103 treated with Cu ions is a subject to catabolite repression.

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Section: Fig 3 Deinduction Of Sod Synthesis By Removal Of Cu Ionscontrasting

confidence: 99%

Section: Fig 3 Deinduction Of Sod Synthesis By Removal Of Cu Ionsmentioning

confidence: 99%

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confidence: 99%

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Regulation of Superoxide Dismutase Synthesis in Humcola lutea Cells under Cu2+ Stress Conditions

Krumova¹,

Abrashev²,

Miteva-Staleva³

et al. 2013

Proceeding BAS

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“…The composition of the seed and production media was as described previously (Angelova et al 1995). Cultivation was performed in 3 L bioreactors, ABR-09, equipped with pH and automatic dissolved oxygen (DO) monitoring equipment and a control system.…”

Section: Methodsmentioning

confidence: 99%

“…-The cell-free extract was prepared as described earlier (Angelova et al 1995). SOD activity was measured by the nitro-blue tetrazolium (NBT) reduction method of Beauchamp and Fridovich (1971).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Kostadinova¹,

Tosi²,

Spassova³

et al. 2017

Polish Polar Research

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Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below -(10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below-and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below-and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.

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Temperature downshift induces antioxidant response in fungi isolated from Antarctica

et al. 2008

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Although investigators have been studying the cold-shock response in a variety of organisms for the last two decades or more, comparatively little is known about the difference between antioxidant cell response to cold stress in Antarctic and temperate microorganisms. The change of environmental temperature, which is one of the most common stresses, could be crucial for their use in the biotechnological industry and in ecological research. We compared the effect of short-term temperature downshift on antioxidant cell response in Antarctic and temperate fungi belonging to the genus Penicillium. Our study showed that downshift from an optimal temperature to 15 degrees or 6 degrees C led to a cell response typical of oxidative stress: significant reduction of biomass production; increase in the levels of oxidative damaged proteins and accumulation of storage carbohydrates (glycogen and trehalose) in comparison to growth at optimal temperature. Cell response against cold stress includes also increase in the activities of SOD and CAT, which are key enzymes for directly scavenging reactive oxygen species. This response is more species-dependent than dependent on the degree of cold-shock. Antarctic psychrotolerant strain Penicillium olsonii p14 that is adapted to life in extremely cold conditions demonstrated enhanced tolerance to temperature downshift in comparison with both mesophilic strains (Antarctic Penicillium waksmanii m12 and temperate Penicillium sp. t35).

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Effect of glucose on the superoxide dismutase production in fungal strainHumicola lutea

Cited by 19 publications

References 19 publications

Regulation of Superoxide Dismutase Synthesis in Humcola lutea Cells under Cu2+ Stress Conditions

Regulation of Superoxide Dismutase Synthesis in Humcola lutea Cells under Cu2+ Stress Conditions

Comparison of the oxidative stress response of two Antarctic fungi to different growth temperatures

Temperature downshift induces antioxidant response in fungi isolated from Antarctica

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