Effect of glutathione on the response of Escherichia coli to penicillin

Influence of Glutathione on the Inhibition of Escherichia coli by Kanamycin**University of California School of Pharmacy San Francisco 22

“…coli ATCC 6522 was maintained as in the work reported previously (1,2 ) by daily transfers in Anderson's chemically defined medium as modified by Cowie, et nl. (5).…”

“…H E ABlLITY of Escherichia coli to survive Tand multiply in the presence of potassium benzyl penicillin may be increased or decreased by the addition of glutathione (GSH). The direction and the magnitude of the change depend on the absolute concentration of penicillin and on the relative concentrations of the antibiotic and the GSH with respect to each other (1). Apparently the complete GSH complement is required to produce the phenomenon, since the constituent moieties of GSH, whether supplied separately or together in the same ratio as in the complete molecule, do not reproduce .the effect (2).…”