Effect of glycinebetaine on metabolite profiles of cold-stored strawberry revealed by 1H NMR-based metabolomics

Comparative Metabolomic Analysis of the Nutritional Aspects from Ten Cultivars of the Strawberry Fruit

“…Organic acids, amino acids, vitamins, other aromatic substances, and nutrients have been reported as the main determinants of strawberry quality and flavor [ 2 ]. For example, strawberry sweetness is determined by sugar, whereas acidity and umami are regulated by organic acids [ 3 ]. The nutrients and bioactive compounds in strawberries, such as vitamins and phenolic constituents, carry benefits and biological functions, such as antioxidation, anti-inflammatory, and anticancer [ 4 ].…”

1H NMR-Based Metabolic Profiling to Follow Changes in Pomelo Cultivars during Postharvest Senescence

“…The experimental conditions of 1 H NMR and 2D NMR spectra were conducted according to our previous methods. 1 H NMR spectra were recorded at 500.13 MHz proton frequency on a BRUKER AVANCE III 500 spectrometer (Bruker, Karlsruhe, Germany) equipped with a TCI cryoprobe and Z-gradient system at 297 K. For signal assignment purposes, 2D NMR spectra were acquired at 25 • C, including 1 H-1 H correlation spectroscopy (COSY), selective total correlation spectroscopy (1D-TOCSY), J-resolved spectroscopy (JRES), 1 H- 13 C heteronuclear single quantum coherence spectroscopy (HSQC) and heteronuclear multiple bond correlation (HMBC). For COSY and 1D-TOCSY experiments, 128 transients were collected into 1024 data points for each of the 160 increments, with a spectral width of 12.0 ppm for both dimensions.…”

“…A phase-insensitive mode with gradient selection was used for the COSY experiments and MLEV-17 was employed as the spin-lock scheme in the phase-sensitive 1D-TOCSY experiment with a mixing time of 80 ms. For JRES spectra, 128 transients were collected into 4096 data points for each of the 80 increments with a spectral width of 6000 Hz in the acquisition and 60 Hz in the evolution dimensions. 1 H- 13 C HSQC and HMBC experiments were recorded by using the gradient selected sequences with 512 transients and 2048 data points for each of the 128 increments. The spectral widths were set at 6000 Hz for 1 H, 20,625 Hz for 13 C in HSQC experiments and 27,500 Hz for 13 C HMBC experiments, respectively.…”

“…1 H- 13 C HSQC and HMBC experiments were recorded by using the gradient selected sequences with 512 transients and 2048 data points for each of the 128 increments. The spectral widths were set at 6000 Hz for 1 H, 20,625 Hz for 13 C in HSQC experiments and 27,500 Hz for 13 C HMBC experiments, respectively. The data were zero-filled to a 2000 × 2000 matrix with appropriate window functions prior to Fourier transformation.…”

“…For the advantages of speed and simplicity of samples preparation, information on primary and secondary metabolites can be obtained simultaneously, with high reproducibility and non-destruction. 1 H-NMR has also been widely recognized as an effective tool to display its promising potential in metabolites analysis of foods and beverages [11][12][13][14]. Subsequently, the NMR data are frequently processed by multivariate data analysis, such as principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) has usually been utilized to screen the crucial biomarkers between samples [15,16].…”