Effect of glycylglycine and glycine on jejunal absorption rate of L‐histidine in man <i>in vivo</i>

Cook, Gordon

doi:10.1113/jphysiol.1974.sp010477

Cited by 10 publications

(8 citation statements)

References 12 publications

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“…Table 2 summarizes the reproducibility of the results for the three 10 min collections during each perfusion (Cook, 1974a). Figure 1 summarizes absorption rate of L-histidine from solutions A, B, and D. Mean absorption rate from solution B is significantly lower than that from solution A (t = 4 100; d.f = 5; p < 001; paired t test).…”

Section: Procedures and Investigationsmentioning

confidence: 98%

“…Determinationswere made on all perfusion solutions and samples of intestinal content obtained during those perfusions, at the same time; glycine and glycylglycine were estimated in triplicate, and L-histidine (free or in the form of carnosine) and PEG in duplicate. Table 2 Reproducibility of absorption rates for three 10-minute collections of intestinal content during each perfusion *Cook (1974a).…”

Section: Procedures and Investigationsmentioning

confidence: 99%

“…That applies both to experimental animals Smyth, 1959, 1960;Matthews et al, 1968;Matthews, 1971 and man Adibi, 1971;Cook, 1972Cook, , 1973Cook, , 1974aHellier et al, 1972).…”

mentioning

confidence: 99%

“…4) was clinically malnourished. Methods for serum protein determination and Dxylose absorption have been described by Cook (1974a).…”

mentioning

confidence: 99%

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Impairment of jejunal absorption rate of carnosine by glycylglycine in man in vivo.

Cook¹

1976

Gut

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SUMMARY Using a double-lumen tube jejunal perfusion system in vivo, the mutual effects of carnosine (P-alanyl-L-histidine) and glycylglycine on their respective absorption rates have been studied in six Zambian African adults. Data on the effect of the constituent amino-acids of carnosine on glycylglycine absorption rate have similarly been obtained. The solutions infused in each subject contained (A) carnosine (50 mmol 1.-i), (B) carnosine (50 mmol 1.-i) and glycylglycine (50 mmol 1.-1), (C) glycylglycine (50 mmol 1.-), and (D) glycylglycine (50 mmol 1.-1), L-histidine (50 mmol 1.1) and 3-alanine (50 mmol 1.-1). Glycylglycine produced a significant impairment in the mean rate of histidine absorption from carnosine (p < 0-01). However, carnosine did not have a significant effect on the mean rate of glycine absorption from glycylglycine. Mean rate of histidine absorption from solution D was significantly higher than that from solution A (p < 0 01). Mean rate of glycine absorption from glycylglycine was not significantly different during infusion of solutions B, C, and D. The results are consistent with the concept that carnosine and glycylglycine share the same transfer mechanism; the lack of influence of carnosine on glycylglycine is probably because the affinity of carnosine for the dipeptide uptake mechanism is relatively low. A gross difference has been shown between mean absorption rate of histidine from free L-histidine (solution D) (25.8 mmol h-1) and when it is given in the form of carnosine in the presence of another dipeptide (solution B) (8-7 mmol h-1); that emphasizes the complexity of amino acid and peptide interaction during absorption, which must be important in nutrition.Dipeptides and their constituent amino acids are absorbed at different rates and use transfer mechanisms that are partly and possibly wholly separate.

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Section: Procedures and Investigationsmentioning

confidence: 98%

Section: Procedures and Investigationsmentioning

confidence: 99%

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Impairment of jejunal absorption rate of carnosine by glycylglycine in man in vivo.

Cook¹

1976

Gut

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“…Results of in vitro and in vivo experiments carried out in animals and man indicate that separate transport processes may be involved in the mucosal uptake of peptide bound and free amino acids (Matthews, 1971;Milne, 1971; Cook, 1974;Silk, 1974a). Recent evidence suggests that there may be two modes of uptake of amino acids from oligopeptides: (1) surface hydrolysis by mechanisms closely linked to the specific free amino acid entry mechanisms, and (2) entry of intact peptides into the mucosal cells A. E. LANE, D. B.…”

Section: Introductionmentioning

confidence: 99%

Absorption of two proline containing peptides by rat small intestine in vivo.

Lane¹,

Silk²,

Clark³

1975

The Journal of Physiology

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SUMMARY1. Glycyl-L-proline and L-prolyl-glycine are two dipeptides that are poorly hydrolysed by brush border peptide hydrolases in vitro. The present study was undertaken to investigate in vivo intestinal absorption of the two dipeptides in male Wistar rats. A steady-state perfusion technique has been used and jejunal and ileal absorption studied by comparing rates of absorption of constituent amino acids from dipeptide and equivalent equimolar amino acid solutions.2. Glycine and proline were absorbed at the same or faster rates during perfusion of 10 mM glycyl-L-proline than during perfusion of its equivalent equimolar amino acid solution. Conversely, the two constituent amino acids were absorbed at slower rates during perfusion of 10 mM-L-prolylglycine than during perfusion of its equivalent amino acid solution. As expected very low concentrations of free amino acids were detected during the jejunal dipeptide perfusions but higher concentrations were detected during the ileal perfusions.3. The addition of 40 mM L-prolyl-glycine to the 10 mM glycyl-L-proline solution did not affect the rates of disappearance of glycyl-L-proline. The addition of 40 mM glycyl-L-proline to 10 mM L-prolyl-glycine however had a significant inhibitory effect on the disappearance of L-prolyl-glycine. It is concluded that the two dipeptides may be absorbed by different peptide transport systems.

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Dipeptide Transport in the Intestinal Mucosa of Developing Rabbits

Rubino

Guandalini

1977

Novartis Foundation Symposia

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Effect of glycylglycine and glycine on jejunal absorption rate of L‐histidine in man in vivo

Cited by 10 publications

References 12 publications

Impairment of jejunal absorption rate of carnosine by glycylglycine in man in vivo.

Impairment of jejunal absorption rate of carnosine by glycylglycine in man in vivo.

Absorption of two proline containing peptides by rat small intestine in vivo.

Dipeptide Transport in the Intestinal Mucosa of Developing Rabbits

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