Effect of gonadotropins on ovulation and ovarian histology in the immature mongolian gerbil

Fischer, Theodore V.; Fisher, Donald

doi:10.1002/aja.1001420308

Cited by 6 publications

(7 citation statements)

References 4 publications

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“…Conversely, the mean number of ova obtained in ovulated females was stable at around 30 when the interval between PMSG and hCG injections was 54 hours or more. This mean number of ovulated ova is comparable to the results of other investigators [1][2][3]. Mature female Mongolian gerbils have an estrus cycle of 4-6 days [7], a mean number of 3.9 pups (range 1-9) [8] and a mean number of ovulated ova of 6.6 [1] in their natural cycle.…”

Section: Discussionsupporting

confidence: 77%

“…Ovulation, fertilization, and early cleavage in the Mongolian gerbil were investigated by Marston et al in the 1960s [1]. The protocol for inducing superovulation in the Mongolian gerbil has been examined by Mastron and Chang [1], Fisher and Fisher [2], and Ishijima et al [3]. Mastron and Chang [1] injected mature females with 10 to 40 IU PMSG (pregnant mare's serum gonadotropin) followed by 10 IU hCG (human chorionic gonadotropin) 52 hours later.…”

Section: Introductionmentioning

confidence: 99%

“…They observed a maximal ovulation of 39.0 ova per animal with a combination of 40 IU PMSG and 10 IU hCG. Fisher and Fisher [2] injected immature females with 10 to 20 IU PMSG followed by 5 to 20 IU hCG 50 hours later. They proposed that the combination of 10 IU PMSG and 10 IU hCG was the optimal dosage for superovulation in immature females.…”

Section: Introductionmentioning

confidence: 99%

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Interval between PMSG Priming and hCG Injection in Superovulation of the Mongolian Gerbil

2004

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interval between PMSG Priming and hCG Injection in Superovulation of the Mongolian Gerbil

2004

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“…Thus, it seems that 8 IU PMSG is the best concentration among the different doses of PMSG that were studied in this research. Based on the study by Fischer and Fisher [ 40 ], the best superovulatory doses of PMSG on the rate and time of ovulation and ovarian histology in gerbils was observed with 10 IU PMSG. Based on the above consideration, we concluded that the optimal dose for superovulation and less alteration in uterine glycoconjugates of rats at implantation time was induced by the administration of 8-10 IU PMSG.…”

Section: Discussionmentioning

confidence: 99%

Histochemical Study of the Rat Uterine Glycoconjugate Alteration following Treatment with Exogenous Gonadotropic Hormones during the Implantation Period

Aliabadi

Makoolati

Talaei‐Khozani

et al. 2020

BioMed Research International

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One of the female causes of infertility is anovulation which is treatable with gonadotropin hormones. These hormones affect the molecular organization of the uterus such as glycoconjugates that are the first site of contact between the blastocyst and the uterus. The objective of this project was to study the alteration of glycoconjugates on the uterine apical, Golgi zone, and basement membrane of epithelial cells and the uterine gland after hyperstimulation with pregnant mare serum gonadotropin (PMSG) (4, 8, 16, 24, and 40 IU), during the implantation period. Injection of PMSG (in experimental groups) and injection of distilled water (in the control group) were followed by HCG administration (10 IU), mating, isolation of positive vaginal plug rats, and killing at 5.5 days of pregnancy. Histochemistry was done on the pregnant uterine horns with the use of WGA, DBA, PNA, ConA, SBA, and UEA lectins. The intensity of the immunohistochemical staining was scored, and quantitative data were generated. 4 IU did not show any significant differences with the control, 8 IU had less effect on the alteration of the Golgi zone, and apical and basement membrane glycoconjugates and 40 IU had the least effects on the alteration of uterine gland glycoconjugates. Also, 24 IU had the most effect on the alteration of uterine glycoconjugates. Understanding of the effects of gonadotropin hormones at the uterine level in implantation time helps to optimize hormonal manipulation for improving the outcome of assisted reproductive procedures. It seems that the optimal dose for superovulation and less alteration in uterine glycoconjugates of rats at implantation time were induced by the administration of 8 IU PMSG.

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“…Approximately 50 h later, 5 IU of hCG were injected intraperitoneally. The females were housed in the original cages for oocytes production or transferred into the male cages right after the hCG injection for mating (modified from (14)).…”

Section: Methodsmentioning

confidence: 99%

Transferring isolated mitochondria into tissue culture cells

Yang

Koob

2012

Nucleic Acids Research

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We have developed a new method for introducing large numbers of isolated mitochondria into tissue culture cells. Direct microinjection of mitochondria into typical mammalian cells has been found to be impractical due to the large size of mitochondria relative to microinjection needles. To circumvent this problem, we inject isolated mitochondria through appropriately sized microinjection needles into rodent oocytes or single-cell embryos, which are much larger than tissue culture cells, and then withdraw a ‘mitocytoplast’ cell fragment containing the injected mitochondria using a modified holding needle. These mitocytoplasts are then fused to recipient cells through viral-mediated membrane fusion and the injected mitochondria are transferred into the cytoplasm of the tissue culture cell. Since mouse oocytes contain large numbers of mouse mitochondria that repopulate recipient mouse cells along with the injected mitochondria, we used either gerbil single-cell embryos or rat oocytes to package injected mouse mitochondria. We found that the gerbil mitochondrial DNA (mtDNA) is not maintained in recipient rho0 mouse cells and that rat mtDNA initially replicated but was soon completely replaced by the injected mouse mtDNA, and so with both procedures mouse cells homoplasmic for the mouse mtDNA in the injected mitochondria were obtained.

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Effect of gonadotropins on ovulation and ovarian histology in the immature mongolian gerbil

Cited by 6 publications

References 4 publications

Interval between PMSG Priming and hCG Injection in Superovulation of the Mongolian Gerbil

Interval between PMSG Priming and hCG Injection in Superovulation of the Mongolian Gerbil

Histochemical Study of the Rat Uterine Glycoconjugate Alteration following Treatment with Exogenous Gonadotropic Hormones during the Implantation Period

Transferring isolated mitochondria into tissue culture cells

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