Effect of Granulocyte and Granulocyte Macrophage Colony Stimulating Factors (G-CSF and GM-CSF) on Neonatal Neutrophil Functions

Wolach, Baruch; Gavrieli, Ronit; Pomeranz, Avishalom

doi:10.1203/00006450-200009000-00018

Cited by 34 publications

(11 citation statements)

References 57 publications

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“…In contrast, G‐CSF stimulation of donors did not have a significant effect on phagocytosis or respiratory burst, but enhanced the chemotactic activity of granulocytes collected. There is no consensus in the literature regarding the effect of G‐CSF stimulation on chemotaxis; some studies suggest it has no effect, whereas others report a reduced or enhanced response (Hogland et al , 1997; Dale et al , 1998; Leavey et al , 1998; Wolach et al , 2000; Joos et al , 2002).…”

Section: Discussionmentioning

confidence: 99%

Neutrophil function is preserved in a pooled granulocyte component prepared from whole blood donations

Bashir¹,

Stanworth²,

Massey³

et al. 2008

Br J Haematol

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Summary Whole blood‐derived granulocytes (buffy coats) are issued as an alternative to apheresis donations, but are heavily contaminated with red cells and platelets and there is minimal in vitro data describing their functionality. We developed a purer pooled granulocyte component (PGC) from whole blood donations by pooling 10 ABO‐matched buffy coats with 400 ml of platelet additive solution (SSP+) and re‐centrifuging. The PGC was irradiated (25–50 Gy) and neutrophil viability, chemotaxis, phagocytosis and respiratory burst activity were determined by flow cytometry. Results from 13 PGC at 16–20 h following donation were compared with those obtained from 20 standard individual buffy coats and with fresh whole blood. The PGC contained similar numbers of neutrophils (approximately 0·9 × 1010) with a reduced volume and haemoglobin content when compared with 10 individual buffy coats. Neutrophils in the PGC maintained >90% viability, oxidative burst and phagocytic activity and their ability to migrate towards a chemoattractant 16–20 h following donation, which is similar to results obtained with either fresh whole blood or standard buffy coats. Therefore, neutrophil function in the PGC was preserved 16–20 h following donation, but this product had significantly lower red cell contamination compared with 10 buffy coats, which are currently transfused.

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Section: Discussionmentioning

confidence: 99%

Neutrophil function is preserved in a pooled granulocyte component prepared from whole blood donations

Bashir¹,

Stanworth²,

Massey³

et al. 2008

Br J Haematol

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“…Additionally, although there is evidence for a positive correlation between low levels of superoxide anion and bactericidal activity (Boxer, 1995), there is no evidence that superoxide overproduction has such an effect on bactericidal activity (Sirota et al, 2003). In fact, Wolach et al (2000) have shown a high superoxide generation by neutrophils with no parallel effect on their bactericidal activity. Thus, acupuncture could exert its immunomodulatory action, at least in part, by decreasing the oxidative stress condition of immune cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Acupuncture Treatment on the Immune Function Impairment Found in Anxious Women

et al. 2007

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It is presently accepted that emotional disturbances lead to immune system impairment, and that therefore their treatment could restore the immune response. Thus, the aim of the present work was to study the effect of an acupuncture treatment, designed specifically to relieve the emotional symptoms stemming from anxiety, on several functions (adherence, chemotaxis, phagocytosis, basal and stimulated superoxide anion levels, lymphocyte proliferation in response to phytohemagglutinin A (PHA) and natural killer (NK) activity) of leukocytes (neutrophils and lymphocytes) from anxious women. The acupuncture protocol consisted of manual needle stimulation of 19 acupoints, with each session lasting 30 min. It was performed on 34 female 30-60 year old patients, suffering from anxiety, as determined by the Beck Anxiety Inventory (BAI). Before and 72 hours after receiving the first acupuncture session, peripheral blood samples were drawn. In 12 patients, samples were also collected immediately after the first single acupuncture session and one month after the end of the whole acupuncture treatment, which consisted of 10 sessions during a year, until the complete remission of anxiety. Twenty healthy non-anxious women in the same age range were used as controls. The results showed that the most favorable effects of acupuncture on the immune functions appear 72 hours after the single session and persist one month after the end of the complete treatment. Impaired immune functions in anxious women (chemotaxis, phagocytosis, lymphoproliferation and NK activity) were significantly improved by acupuncture, and augmented immune parameters (superoxide anion levels and lymphoproliferation of the patient subgroup whose values had been too high) were significantly diminished. Acupuncture brought the above mentioned parameters to values closer to those of healthy controls, exerting a modulatory effect on the immune system.

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“…Wolach et al (41) reported that prior exposure to GM-CSF primes neutrophils, resulting in enhanced polarization, chemotaxis, and reactive oxygen species production in response to subsequent fMLP stimulation. GM-CSF has also been reported as a direct chemoattractant for neutrophils, albeit at a higher concentration (125 pg/ml) than that found in our conditioned media (ϳ5 pg/ml) (34).…”

Section: Discussionmentioning

confidence: 99%

“…Some members of these families are known to promote neutrophil chemotaxis (12,25), whereas the majority of cytokines/chemokines produced by skeletal muscle cells have yet to be tested for their potential to induce neutrophil chemotaxis. Granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-1, IL-6, IL-8, monocyte chemotactic protein (MCP)-1, macrophage inflammatory protein (MIP)-1␣, and TNF-␣ have the potential to directly or indirectly influence neutrophil chemotaxis after mechanical loading of skeletal muscle because they are known to influence various aspects of the inflammatory response (7,41). Little is known, however, about how mechanical loading of skeletal muscle cells influences the production and/or release of these and other cytokines.…”

mentioning

confidence: 99%

Cytokines derived from cultured skeletal muscle cells after mechanical strain promote neutrophil chemotaxis in vitro

Peterson¹,

Pizza²

2009

Journal of Applied Physiology

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We tested the hypothesis that cytokines derived from differentiated skeletal muscle cells in culture induce neutrophil chemotaxis after mechanical strain. Flexible-bottom plates with cultured human muscle cells attached were exposed to mechanical strain regimens (ST) of 0, 10, 30, 50, or 70 kPa of negative pressure. Conditioned media were tested for the ability to induce chemotaxis of human blood neutrophils in vitro and for a marker of muscle cell injury (lactate dehydrogenase). Conditioned media promoted neutrophil chemotaxis in a manner that was related both to the degree of strain and to the magnitude of muscle cell injury (ST 70 > ST 50 > ST 30). Protein profiling using a multiplex cytokine assay revealed that mechanical strain increased the presence of IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor, monocyte chemotactic protein (MCP)-1, and IL-6 in conditioned media. We also detected 14 other cytokines in conditioned media from control cultures that did not respond to mechanical strain. Neutralization of IL-8 and GM-CSF completely inhibited the chemotactic response for ST 30 and ST 50 and reduced the chemotactic response for ST 70 by 40% and 47%, respectively. Neutralization of MCP-1 or IL-6 did not reduce chemotaxis after ST 70. This study enhances our understanding of the immunobiology of skeletal muscle by revealing that skeletal muscle cell-derived IL-8 and GM-CSF promote neutrophil chemotaxis after injurious mechanical strain.

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Effect of Granulocyte and Granulocyte Macrophage Colony Stimulating Factors (G-CSF and GM-CSF) on Neonatal Neutrophil Functions

Cited by 34 publications

References 57 publications

Neutrophil function is preserved in a pooled granulocyte component prepared from whole blood donations

Neutrophil function is preserved in a pooled granulocyte component prepared from whole blood donations

Effect of Acupuncture Treatment on the Immune Function Impairment Found in Anxious Women

Cytokines derived from cultured skeletal muscle cells after mechanical strain promote neutrophil chemotaxis in vitro

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