Effect of granulocyte chalone on acute and chronic granulocytic leukaemia in man. Report of seven cases

Rytömaa, T.; Ja, Vilpo; Levanto, A; Wa, Jones

doi:10.1111/j.1600-0609.1976.tb01462.x

Cited by 10 publications

(4 citation statements)

References 14 publications

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“…This is the first report where the equivalency between human and animal bone marrow in vivo and mouse bone marrow in vitro has been confirmed. The present status regarding the granulocyte chalone may be expressed using Rytomaa's words: 'The most critical aspects of the chalone concept have been the inconclusive experimental demonstration of the absolute specificity, and that fact that no one has been able to isolate any of these substances in a chemically pure form' (Rytomaa 1976). This applies well to the present test substance too.…”

Section: Discussionmentioning

confidence: 80%

“…From the theoretical point of view, the absolute specificity is not a strict prerequisite for a substance as being a chalone (cf. Rytomaa 1976). I have not forgotten the possibility that the present test preparation is not chalonal at all.…”

Section: Discussionmentioning

confidence: 84%

“…from 500 to 10000 daltons was isolated by ultrafiltration, and the appropriate fractions were desalted, lyophilized, further purified by gel filtration chromatography (and the appropriate fractions were desalted, lyophilized) and conserved in -20° C. Two preparations, obtained from roughly the same number of leucocytes were used. Some of the properties of this kind of extract have been discussed previously (Rytomaa et al 1976). The chemical and functional characterization of the test extract is in progress.…”

Section: The Leucocyte Extractmentioning

confidence: 99%

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Non‐Specific and Specific Effects of a Granulocytic Bio‐Inhibitor on Different Haematopoietic Cell Populations

Vilpo

1979

Scandinavian Journal of Haematology

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The effect of a leucocyte conditioned medium derivative on the 5‐125iodo‐2′‐deoxy‐uridine (125I‐UdR) uptake by 6 haematopoietic cell populations with a simple in vitro assay system was tested. The low doses of the test substance ‐ a putative granulocytic chalone ‐ inhibited significantly the uptake of 125I‐UdR only in predominantly granulocytic cell cultures. This inhibition was achieved using μg/ml concentrations, which have previously been shown to be inhibitory to normal and malignant granulocytic cells in vivo. The test preparations inhibited the uptake of 125I‐UdR in all cell populations (non‐granulocytic included) when doses exceeding 10 μg/ml were used. The prerequisite for an adequate demonstration of the specificity of a bio‐inhibitor is discussed.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 84%

Section: The Leucocyte Extractmentioning

confidence: 99%

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Non‐Specific and Specific Effects of a Granulocytic Bio‐Inhibitor on Different Haematopoietic Cell Populations

Vilpo

1979

Scandinavian Journal of Haematology

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“…Both the fraction of phagocytosing leukocytes and the number of prey attached or internalized per phagocyte were measured (12)(13)(14). 16.88 (n = 11). No significant age variations or differences throughout the months of January to October were found.…”

Section: Phagocytosis Assaymentioning

confidence: 99%

A synthetic hemoregulatory peptide (HP5B) inhibits human myelopoietic colony formation (CFU‐GM) but not leukocyte phagocytosis in vitro

Lærum

Sletvold

Bjerknes

et al. 1987

European J of Haematology

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A synthetic analog of a hemoregulatory peptide associated with mature human granulocyte (HP5B) has been investigated for inhibitory effects on human myelopoietic stem cells in vitro. In addition, it has been tested for effects on phagocytosis by human granulocytes and monocytes by use of an automatic flow cytometric method. A dose-dependent inhibition of colony formation was found after preincubation of bone marrow cells for 1 h at 37'C in the range 10-7-10-" mol/l. Above or below these concentrations, no inhibitory effects were seen. The degree of inhibition varied from experiment to experiment, indicating variable responsiveness of the donor cells. Maximal effect was of magnitude 90% inhibition, and the optimal dose was lo-' mol/l. The peptide had no effect on the kinetics of phagocytosis by measurements of the uptake of fluorescent Zymosan particles or Staphylococcus aureus. This may indicate a selective effect on the precursor cells, with no effect on the functional state of their progeny, the granulocytes and monocytes.In a series of recent publications we have reported the isolation and synthesis of a pentapeptide associated with mature human granulocytes which has a selective inhibititory effect on myelopoiesis both in vitro and in vivo (1-5). The most striking biological effect was a strong suppression of the myelopoietic stem cells in mouse bone marrow in vivo (6). In addition, the effect was non-toxic and reversible, which could indicate that the peptide had functions as a physiological regulator.However, several questions remained open. So 17.

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Gewebsspezifische Proliferationshemmung lymphoider Zellen in vitro und in vivo mit Kalbsmilzextrakt (Lymphozytenchalon)

Heidemann¹,

Ganser²,

Podgornik³

et al. 1977

Verhandlungen Der Deutschen Gesellschaft Für Innere Medizin

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Effect of granulocyte chalone on acute and chronic granulocytic leukaemia in man. Report of seven cases

Cited by 10 publications

References 14 publications

Non‐Specific and Specific Effects of a Granulocytic Bio‐Inhibitor on Different Haematopoietic Cell Populations

Non‐Specific and Specific Effects of a Granulocytic Bio‐Inhibitor on Different Haematopoietic Cell Populations

A synthetic hemoregulatory peptide (HP5B) inhibits human myelopoietic colony formation (CFU‐GM) but not leukocyte phagocytosis in vitro

Gewebsspezifische Proliferationshemmung lymphoider Zellen in vitro und in vivo mit Kalbsmilzextrakt (Lymphozytenchalon)

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