Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation

Zaccaria, Vincenzo; Curti, Valeria; Lorenzo, Arianna Di; Baldi, Alessandra; Maccario, Cristina; Sommatis, Sabrina; Mocchi, Roberto; Daglia, Maria

doi:10.3390/nu9101090

Cited by 49 publications

(43 citation statements)

References 45 publications

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“…Ibuprofen did not show any activity in this test. In recent years, various investigations have undertaken the study of propolis as an antioxidant of natural origin for the prevention and treatment of various diseases of oxidative origin 42 .…”

Section: Issn: 2250-1177mentioning

confidence: 99%

Studies of the Biological and Therapeutic Effects of Argentine Stingless Bee Propolis

Brodkiewicz

Marcinkevicius

Reynoso

et al. 2018

J. Drug Delivery Ther.

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Meliponids are native bees of the Americas, characterized by having no sting (ANSA). Some live in the Yunga forests of northern Argentina, a place rich in a diversity of this type of bees of which at least thirty-three species may be found. Propolis is a resinous substance that bees collect from plants exudates. Chemical composition and functional properties vary according to the flora of the place where the hives are. They have been valued by humans for their biological properties for centuries. This study is aimed at investigating the antinociceptive, antioxidant and anti-biofilm activities of propolis from the stingless bees Tetragonisca fiebrigi Schwarz and Scaptotrigona jujuyensis Schrottky found in Estación Experimental Agropecuaria Famaillá of INTA, Tucumán, Argentina. Analgesic activity of the extracts was estimated against acetic acid induced writhing, tail immersion method and formalin test. Antioxidant capacity was evaluated using DPPH free radical scavenging and β-carotene bleaching assays. Propolis anti biofilm activity was tested on Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATTC 6538P bacteria. Phytochemical constituents were obtained using standard chemical methods. The propolis alcoholic extracts of the studied species possess antinociceptive activity at both central and peripheral levels as demonstrated by the three algesia tests used. Both propolis extracts were effective antioxidants in DPPH and β-carotene linoleic acid model systems. S. jujuyensis propolis tested at all doses against S. aureus and P. aeruginosa presented a selective biofilm inhibition unrelated to bacterial growth inhibition, thus achieving a reduction in pathogenicity. The chemical studies revealed the presence of sterols, triterpenes, catechins, coumarins, flavonoids, phenols, tannins and anthocyanidins. Chemical composition observed in the T.fiebrigi and S. jujuyensis propolis, suggest that those responsible for the activity would be chemical compounds of a non-phenolic nature. Our data indicate that geopropolis is a natural source of bioactive substances with promising beneficial properties for human health. Isolation and identification of compounds responsible for the pharmacological activities displayed by propolis has started.

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Section: Issn: 2250-1177mentioning

confidence: 99%

Studies of the Biological and Therapeutic Effects of Argentine Stingless Bee Propolis

Brodkiewicz

Marcinkevicius

Reynoso

et al. 2018

J. Drug Delivery Ther.

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“…Propolis is a product derived from resins and plant exudates; its composition varies depending on the geographic region, flora, and local climate; and it is used by bees to protect the hive against macro- and micro-invaders [16, 18, 19]. Because of its specific chemical and biological characteristics, propolis has been used for hundreds of years by various peoples around the world, with diverse cultures, for medicinal purposes [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of Brazilian green propolis on proteinuria and renal function in patients with chronic kidney disease: a randomized, double-blind, placebo-controlled trial

et al. 2019

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Background Chronic kidney disease (CKD) is a public health problem worldwide, and proteinuria is a well-established marker of disease progression in CKD patients. Propolis, a natural resin produced by bees from plant materials, has anti-inflammatory, immunomodulatory, and anti-oxidant properties, as well as having been shown to have an antiproteinuric effect in experimental CKD. The aim of this study was to evaluate the impact of Brazilian green propolis extract on proteinuria reduction and the changes in the estimated glomerular filtration rate (eGFR). Methods This was a randomized, double-blind, placebo-controlled study including patients with CKD caused by diabetes or of another etiology, 18–90 years of age, with an eGFR of 25–70 ml/min per 1.73 m 2 and proteinuria (urinary protein excretion > 300 mg/day) or micro- or macro-albuminuria (urinary albumin-to-creatinine ratio > 30 mg/g or > 300 mg/g, respectively). We screened 148 patients and selected 32, randomly assigning them to receive 12 months of Brazilian green propolis extract at a dose of 500 mg/day ( n = 18) or 12 months of a placebo ( n = 14). Results At the end of treatment, proteinuria was significantly lower in the propolis group than in the placebo group—695 mg/24 h (95% CI, 483 to 999) vs. 1403 mg/24 h (95% CI, 1031 to 1909); P = 0.004—independent of variations in eGFR and blood pressure, which did not differ between the groups during follow-up. Urinary monocyte chemoattractant protein-1 was also significantly lower in the propolis group than in the placebo group—58 pg/mg creatinine (95% CI, 36 to 95) vs. 98 pg/mg creatinine (95% CI, 62 to 155); P = 0.038. Conclusions Brazilian green propolis extract was found to be safe and well tolerated, as well as to reduce proteinuria significantly in patients with diabetic and non-diabetic CKD. Trial Registration. ( ClinicalTrials.gov number NCT02766036. Registered: May 9, 2016). Electronic supplementary material The online version of this article (10.1186/s12882-019-1337-7) contains supplementary material, which is available to authorized users.

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“…All the above-mentioned reports strongly suggest that miRs play an important role in the process of I/R injury. Recently, the broadly conserved miR-19a-3p, a crucial component of the miR-17-92 cluster, has been shown to be a mediator of the cell proliferation-inhibitory effect in breast cancer [13], cell apoptosis in chemosensitivity of osteosarcoma [14] and inflammatory responses [15]. Interestingly, our attention was aroused by miR-19a-3p which is the most widely modulated miRNA as novel biomarker in ischemic stroke by Eyileten et al [16].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro

et al. 2020

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Background: Inflammation and apoptosis are considered to be two main factors affecting ischemic brain injury and the subsequent reperfusion damage. MiR-19a-3p has been reported to be a possible novel biomarker in ischemic stroke. However, the function and molecular mechanisms of miR-19a-3p remain unclear in cerebral ischemia/reperfusion (I/R) injury. Methods: The I/R injury model was established in vivo by middle cerebral artery occlusion/reperfusion (MCAO/R) in rats and in vitro by oxygen-glucose deprivation and reperfusion (OGD/R) induced SH-SY5Y cells. The expression of miR-19a-3p was determined by reverse transcription quantitative PCR. The infarction volumes, Neurological deficit scores, apoptosis, cell viability, pro-inflammatory cytokines and apoptosis were evaluated using Longa score, Bederson score, TTC, TUNEL staining, CCK-8, ELISA, flow cytometry assays. Luciferase reporter assay was utilized to validate the target gene of miR-19a-3p. Results: We first found miR-19a-3p was significantly up-regulated in rat I/R brain tissues and OGD/R induced SH-SY5Y cells. Using the in vivo and in vitro I/R injury model, we further demonstrated that miR-19a-3p inhibitor exerted protective role against injury to cerebral I/R, which was reflected by reduced infarct volume, improved neurological outcomes, increased cell viability, inhibited inflammation and apoptosis. Mechanistically, miR-19a-3p binds to 3′UTR region of IGFBP3 mRNA. Inhibition of miR-19a-3p caused the increased expression of IGFBP3 in OGD/R induced SH-SY5Y cells. Furthermore, we showed that IGFBP3 overexpression imitated, while knockdown reversed the protective effects of miR-19a-3p inhibitor against OGD/R-induced injury. Conclusions: In summary, our findings showed miR-19a-3p regulated I/R-induced inflammation and apoptosis through targeting IGFBP3, which might provide a potential therapeutic target for cerebral I/R injury.

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Effect of Green and Brown Propolis Extracts on the Expression Levels of microRNAs, mRNAs and Proteins, Related to Oxidative Stress and Inflammation

Cited by 49 publications

References 45 publications

Studies of the Biological and Therapeutic Effects of Argentine Stingless Bee Propolis

Studies of the Biological and Therapeutic Effects of Argentine Stingless Bee Propolis

Effects of Brazilian green propolis on proteinuria and renal function in patients with chronic kidney disease: a randomized, double-blind, placebo-controlled trial

Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro

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