Effect of Green Coffee on Cisplatin Induced Renal Apoptosis in Adult Male Albino Rats

Abstract

Background: Green coffee as a functional food has an antioxidant effect, which can reduce the cancer incidence, promote weight-loss and improve glucose and lipid metabolism, as well as anti-oxidant and anti-inflammatory activity. Objective: Assessing the effect of green coffee administration on experimental cisplatin induced renal apoptosis in adult male albino rats of local strain. Design: Randomized Block Design was used. Materials and Methods: Forty adult male albino rats of local strain were randomly divided into four groups of 10 animals each. G1: control negative group was received isotonic saline (0.5 ml, i.p.) for 30 consecutive days, G2: sham operated group mice were received green coffee extract in water (40 mg/kg/day) for 30 consecutive days, animals of G3 and G4 were administered cisplatin (8 mg/kg/day, i.p.) at 10th day, which is well known to produce significant nephrotoxicity in rats. Animals in G3 were received green coffee extract in water (40 mg/kg/day) and G4 was received isotonic normal saline (0.5 ml, i.p.) for 30 consecutive days. All rats were sacrificed after 30 days and blood was withdrawn for biochemical examinations of kidney function tests (blood urea nitrogen, creatinine and uric acid). Kidneys were removed for determination of renal oxidative stress markers (H 2 O 2) biochemically and caspase-3 by Immunohistochemical examination. Results: Cisplatin administration was associated with significant higher levels of BUN, creatinine, uric acid and H 2 O 2 as compared with normal control group. Green coffee administration in cisplatin-induced renal apoptosis groups produced significant lower levels of BUN, creatinine, uric acid and H 2 O 2 (24.4 ± 4.14, 1.730 ± 0.2830, 5.50 ± 0.850 and 0.51 ± 0.12 respectively) as compared with cisplatin-induced renal apoptosis group not administrated green coffee (27.4 ± 6, 2.04 ± .31, 7.00 ± 1.25 and 1.1 ± 0.16 respectively). Cisplatin administration increased expression of the apoptotic protein caspase-3.

Green coffee bean extract attenuates gentamicin induced acute nephrotoxicity in rats

“…The present study showed that oral GCBE administration caused significant regression of renal histopathologic changes when compared to GIAN model group. In accordance with our results Nour El-Deen et al (2019), reported attenuation of renal apoptosis after administration of GCBE to rats. Such improvement was attributed according to the researchers to the significant proven antioxidant effect of GCBE.…”

“…The proven antioxidant effect of GCBE on other injuries of the kidneys was previously reported. Noureldeen et al demonstrated improvement in renal oxidative stress markers after oral administration of GCBE in rats with cisplation induced renal apoptosis (Nour El-Deen et al, 2019). Also the antioxidant effect of GCBE has been demonstrated in other organs like liver, brain and skin (Nogaim et al, 2020;Pergolizzi et al, 2020).…”

“…Green coffee bean extract (GCBE) derived from unroasted coffee beans has been reported to have multiple beneficial therapeutic effects in both experimental animals and humans. Recent studies demonstrated that consumption of GCBE resulted in reduction of cisplatin-induced renal apoptosis in rats (Nour El-Deen et al, 2019), production of antihypertensive effect (Suzuki et al, 2002;Kozuma et al, 2005), inhibition of fat accumulation (Shimoda et al, 2006), and modulation of glucose metabolism (Blum et al, 2007). Such effects have been ascribed to polyphenolic antioxidants called chlorogenic acids (CGA) which are present abundantly in GCBE (Farah et al, 2008).…”

Evaluation of Renoprotective Effects of Our Locally Grown Green Coffee Beans against Cisplatin-Induced Nephrotoxicity in Swiss Albino Mice

“…In line with this finding, Amin et al reported that rats received oral green coffee bean for seven days with the induction of acute renal failure (ARF) showed significantly lower serum creatinine and serum BUN [24]. Comparable studies by Mansour et al reported that green coffee beans had significantly reduced serum creatinine and serum BUN in cisplatin-challenged mice [40]. ese declines in serum creatinine and serum BUN levels in our study may be due to several factors.…”

The Possible Effects of Green Coffee Bean Extract on Progression of Experimental Diabetes in Different Organs; A Review Article

“…Interestingly, green coffee (100 mg kg −1 ) has a remarkable improvement in the levels of Bax, Casp3, and Bcl-2 compared to the control group. El-Deen et al reported that treatment with green coffee diminished apoptosis and reduced the expression of caspase-3 in the renal tubules 26 .…”