Effect of green tea (Camellia sinensis) extract on the rat diaphragm

Das, Mitali; Vedasiromoni, J. R.; Chauhan, S.P.S.; Ganguly, Dilip

doi:10.1016/s0378-8741(97)00069-x

Cited by 11 publications

(11 citation statements)

References 9 publications

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“…As shown here, the aqueous and hydroalcoholic extracts caused facilitation followed by partial inhibition of the twitch tension. Similar results have been reported for green tea (Camellia sinensis) extracts in rat diaphragm and were reproduced by a crude polyphenol preparation (7). The hydroalcoholic extract of guaçatonga contains flavonoids and phenolic compounds.…”

Section: Neutralization Assayssupporting

confidence: 83%

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)

Oshima-Franco¹,

Alves²,

Andréo-Filho³

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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Numerous plants are used as snakebite antidotes in Brazilian folk medicine, including Casearia sylvestris Swartz, popularly known as guaçatonga. In this study, we examined the action of a hydroalcoholic extract from C. sylvestris on the neuromuscular blockade caused by bothropstoxin-I (BthTX-I), a myotoxin from Bothrops jararacussu venom, in mouse isolated phrenic nerve-diaphragm (PND) preparations. Aqueous (8 and 12 mg/ml, n=4 and 5, respectively) and hydroalcoholic (12 mg/ml, n=12) extracts of the leaves of C. sylvestris caused facilitation in PND preparations followed by partial neuromuscular blockade. BthTX-I (20 µg/ml, n=4) caused 50% paralysis after 65±15 min (mean ± S.E.M). Preincubation (30 min at 37°C) of BthTX-I (20 µg/ml, n=4) with a concentration of the hydroalcoholic extract (4 mg/ml) that had no neuromuscular activity, such as the control (n=5), prevented the neuromuscular blockade caused by the toxin. This protection may be mediated by compounds such as flavonoids and phenols identified by thin-layer chromatography and colorimetric assays.

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Section: Neutralization Assayssupporting

confidence: 83%

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)

Oshima-Franco¹,

Alves²,

Andréo-Filho³

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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“…Up until now, few studies (5,8,10,26,37) have dealt with the effects of GTE on skeletal muscle function. Moreover, to the best of our knowledge, the potential of EGCG in muscle wasting paradigms had never been investigated.…”

Section: Discussionmentioning

confidence: 99%

Green tea extract and its major polyphenol (−)-epigallocatechin gallate improve muscle function in a mouse model for Duchenne muscular dystrophy

Dorchies¹,

Wagner²,

Vuadens³

et al. 2006

American Journal of Physiology-Cell Physiology

114

105

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Duchenne muscular dystrophy is a frequent muscular disorder caused by mutations in the gene encoding dystrophin, a cytoskeletal protein that contributes to the stabilization of muscle fiber membrane during muscle activity. Affected individuals show progressive muscle wasting that generally causes death by age 30. In this study, the dystrophic mdx(5Cv) mouse model was used to investigate the effects of green tea extract, its major component (-)-epigallocatechin gallate, and pentoxifylline on dystrophic muscle quality and function. Three-week-old mdx(5Cv) mice were fed for either 1 or 5 wk a control chow or a chow containing the test substances. Histological examination showed a delay in necrosis of the extensor digitorum longus muscle in treated mice. Mechanical properties of triceps surae muscles were recorded while the mice were under deep anesthesia. Phasic and tetanic tensions of treated mice were increased, reaching values close to those of normal mice. The phasic-to-tetanic tension ratio was corrected. Finally, muscles from treated mice exhibited 30-50% more residual force in a fatigue assay. These results demonstrate that diet supplementation of dystrophic mdx(5Cv) mice with green tea extract or (-)-epigallocatechin gallate protected muscle against the first massive wave of necrosis and stimulated muscle adaptation toward a stronger and more resistant phenotype.

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“…The inhibitory effect of Ca 2 was considered to be due to its interaction with superoxide (O À 2 ) free radicals (Babizhayev, 1988). It is reported that both black tea and green tea release Ca 2 from the sarcoplasmic reticulum (Das et al, 1994(Das et al, , 1997. So the Ca 2 releasing action of tea extract is possibly one of the mechanisms of its antineoplastic effect.…”

Section: S42mentioning

confidence: 99%

Inhibition of tumour growth and inflammation by consumption of tea

et al. 2002

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The antitumour effect of tea was evaluated in the 3-methylcholanthrene (3-MC) induced solid tumour model in mice. Both black and green tea inhibited tumour growth and prevented metastasis. Histopathological study showed that tea treatment was able to reduce malignancy. Superoxide dismutase (SOD), a free radical scavenger, was found to be significantly increased in the serum of mice administered tea. Moreover, tea extracts were able to reduce the level of thiobarbituric acid reactive substance (TBARS) in the sera of mice. Tea extracts (both black and green) also showed antiinflammatory activity in the carrageenan-induced paw oedema model in the rat.

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Effect of green tea (Camellia sinensis) extract on the rat diaphragm

Cited by 11 publications

References 9 publications

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)

Neutralization of the neuromuscular activity of bothropstoxin-i, a myotoxin from Bothrops jararacussu snake venom, by a hydroalcoholic extract of Casearia sylvestris Sw. (guaçatonga)

Green tea extract and its major polyphenol (−)-epigallocatechin gallate improve muscle function in a mouse model for Duchenne muscular dystrophy

Inhibition of tumour growth and inflammation by consumption of tea

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