Effect of growth conditions on cofactor-linked xylose reductase activity in Pachysolen tannophilus

Vancauwenberge, J. E.; Bolen, Paul L.; McCracken, Derek A.; Bothast, Rodney J.

doi:10.1016/0141-0229(89)90005-7

Cited by 10 publications

(3 citation statements)

References 19 publications

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“…The increase in residual xylose and glucose with increasing dilution rate followed Monod kinetics applied to a carbonlimited chemostat (Nielsen and Villadsen, 1994). XR from different xylose-utilizing yeasts exhibits different cofactor preferences (du Preez et al, 1989;Rizzi et al, 1988;Skoog and Hahn-Hägerdal, 1990;Vancauwenberge et al, 1989). In contrast, XDH uses exclusively NAD + (Rizzi et al, 1989).…”

Section: Discussionmentioning

confidence: 99%

Intracellular fluxes in a recombinant xylose-utilizingSaccharomyces cerevisiae cultivated anaerobically at different dilution rates and feed concentrations

Wahlbom

Eliasson

Hahn‐Hägerdal³

2000

Biotechnol. Bioeng.

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A metabolic flux model was constructed for the yeast Saccharomyces cerevisiae comprising the most important reactions during anaerobic metabolism of xylose and glucose. The model was used to calculate the intracellular fluxes in a recombinant, xylose‐utilizing strain of S. cerevisiae (TMB 3001) grown anaerobically in a defined medium at dilution rates of 0.03, 0.06, and 0.18 h−1. The feed concentration was varied from 0 g/L xylose and 20 g/L glucose to a mixture of 15 g/L xylose and 5 g/L glucose, so that the total concentration of carbon source was kept at 20 g/L. The specific uptake of xylose increased with the xylose concentration in the feed and with increasing dilution rate. The excreted xylitol was less than half of the xylose consumed. With increasing xylose concentration in the feed, the fluxes in the pentose phosphate pathway increased, whereas the flux through glycolysis decreased. Under all cultivation conditions, nicotinamide adenine dinucleotide (NADH) was the preferred cofactor for xylose reductase. The model showed that the flux through the reaction from ribulose 5‐phosphate to xylulose 5‐phosphate was very low under all cultivation conditions. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 72: 289–296, 2001.

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Section: Discussionmentioning

confidence: 99%

Intracellular fluxes in a recombinant xylose-utilizingSaccharomyces cerevisiae cultivated anaerobically at different dilution rates and feed concentrations

Wahlbom

Eliasson

Hahn‐Hägerdal³

2000

Biotechnol. Bioeng.

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“…One major hybridizing RNA species was observed. Approximately 1.1 kb in size, this RNA species is large enough to encode a 36 kDa xylose reductase characterized in earlier work Van Cauwenberge et al, 1989). The quantity of the hybridizing mRNA species was also shown to increase (lanes 1 4 ) with time of exposure of P. tannophilus cells to xylose.…”

Section: Uninducedmentioning

confidence: 80%

“…One utilizes NADH/ NADPH as cofactors while the other utilizes only NADPH (Verduyn et al, 1985;Ditzelmuller et al, 1984Ditzelmuller et al, , 1985Van Cauwenberge et al, 1989). These two forms may be different proteins, or a single protein that has undergone differential modification.…”

Section: Introductionmentioning

confidence: 99%