Effect of Growth Hormone Treatment on Lymphocyte Functions in Old Male Rats

Baeza, Isabel; Alvarado, Carmen; Ariznavarreta, C.; Castillo, Carmen; Tresguerres, Jesús A.F.; Fuente, Mónica De la

doi:10.1159/000156471

Cited by 13 publications

(9 citation statements)

References 89 publications

(42 reference statements)

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“…GH therapy in elderly rats is able to decrease fat tissue and to increase lean body mass, besides improving the content of plasma lipids, bone density, stimulating skeletal muscle protein synthesis and possibly inhibiting protein degradation via stimulation of IGF-I (Nass et al 2009;Castillo et al 2005b). A beneficial effect in several parameters related with the immune function, such as chemotaxis, natural killer activity and limphoproliferation has been also observed by our group thus delaying some aspects of immunosenescence (Baeza et al 2008).…”

Section: Introductionsupporting

confidence: 58%

Cardiological aging in SAM model: effect of chronic treatment with growth hormone

et al. 2009

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The purpose of this study was to investigate the effect of aging on different parameters related to inflammation, oxidative stress and apoptosis in hearts from two types of male mice models: senescence-accelerated mice (SAM-P8) and senescence-accelerated-resistant (SAM-R1), and the influence of chronic administration of Growth Hormone (GH) on old SAM-P8 mice. Forty male mice were used. Animals were divided into five experimental groups: two 10 month old untreated groups (SAM-P8/SAM-R1), two 2 month old young groups (SAM-P8/SAM-R1) and one 10 month old group (SAM-P8) treated with GH for 30 days. The expression of tumor necrosis factor-alpha, interleukin 1, interleukin 10, heme oxygenases 1 and 2, endothelial and inducible nitric oxide synthases, NFkB, Bad, Bax and Bcl-2 were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Results were submitted to a two way ANOVA statistical evaluation using the Statgraphics program. Inflammation, as well as, oxidative stress and apoptosis markers were increased in the heart of old SAM-P8 males, as compared to young controls and this situation was not observed in the old SAM-R1 mice. Exogenous GH administration reverted the effect of aging in the described parameters of old SAM-P8 mice. Our results suggest that inflammation, apoptosis and oxidative stress could play an important role in the observed cardiovascular alterations related to aging of SAM-P8 mice and that GH may play a potential protective effect on the cardiovascular system of these animals.

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Section: Introductionsupporting

confidence: 58%

Cardiological aging in SAM model: effect of chronic treatment with growth hormone

et al. 2009

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“…In contrast, in experiments with 24 month old Wistar rats, rhGH replacement therapy (2 mg/kg daily for 10 weeks) significantly improved several lymphocyte functions such as NK cell activity (Baeza, et al, 2008). Interestingly, a previous study confirmed that both total NK cell count and particularly CD16 + NK cell subtype were reduced in GHD patients compared to controls (Sneppen et al, 2002).…”

Section: Discussionmentioning

confidence: 88%

Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males

et al. 2010

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It is reported that in both elite and recreational athletes Growth Hormone (GH) is the most commonly used drug aimed at improving sport performance. Aim: The purpose of the present study was to evaluate the immunomodulatory effects of short-term administration of recombinant human GH (rhGH) in healthy young males. NK cell number, activity and phenotype, T cell number, cytokine production of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ and CD4 + /CD8 + ratio with particular attention to the possible correlation to IGF-I production were investigated. Methodology: 30 healthy young males (27 ± 9 yrs) were randomly assigned to either placebo (n = 15) or drug (rhGH) 1mg/day groups (n = 15) with daily injection for seven days. IGF-I serum concentration and flow cytometry data were generated at baseline (day 0) and days 8, 15, 22 and 29 post injection. Data was analysed using a General Linear Model with repeated measures with a Bonferroni correction factor and significance set at p≤0.05. Results: The T cell cytokine secretion profile indicated a Th1 -Th2 shift. Interestingly, a significant difference (p≤0.05) of IL-10 mean fluorescence is noted from day 15 (P= 35.14; ± 19.93, rhGH = 26.63; ± 16.39) to day 22 (P= 61.32; ± 20.41, rhGH= 74.99; ± 46.91) and to day 29 (P= 101.98; ± 67.25, rhGH= 107.74; ± 122.58). Serum IGF-I concentration (ng/mL) increased significantly (p≤0.01) on day 8 (0.48 ± 0.78) after injections compared to baseline (0.31 ± 0.07) as well at day 15 (0.33 ± 0.06), 22 (0.29 ± 0.05) and 29 (0.29 ± 0.06). In conclusion, the cytokine secretion spectrum is affected by short-term rhGH administration in healthy young males being suggested to have induced CD4 + T lymphocytes production of IL-10.

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“…GH up-regulates the production of NO and TNF-α by murine peritoneal macrophages [83] and IGF-1 stimulates production of TNF-α by human monocytes [91]. GH treatment reverses ageing-related reductions of leucocyte chemotaxis, mitogen-induced lymphoproliferation and NK cell activity in male [93] and female [94] rats, with particularly marked effect in ovariectomized animals. GH treatment reverses ageing-related reductions of leucocyte chemotaxis, mitogen-induced lymphoproliferation and NK cell activity in male [93] and female [94] rats, with particularly marked effect in ovariectomized animals.…”

Section: Other Hormones Of Potential Significancementioning

confidence: 99%

Exploring immunomodulation by endocrine changes in Lady Windermere syndrome

Holt

Miles

Inder

et al. 2019

Clinical and Experimental Immunology

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Lung disease due to nontuberculous mycobacteria (NTM) occurs with disproportionate frequency in postmenopausal women with a unique phenotype and without clinically apparent predisposing factors. Dubbed 'Lady Windermere syndrome', the phenotype includes low body mass index (BMI), tall stature and higher than normal prevalence of scoliosis, pectus excavatum and mitral valve prolapse. Although the pathomechanism for susceptibility to NTM lung disease in these patients remains uncertain, it is likely to be multi-factorial. A role for the immunomodulatory consequences of oestrogen deficiency and altered adipokine production has been postulated. Altered levels of adipokines and dehydroepiandrosterone have been demonstrated in patients with NTM lung disease. Case reports of NTM lung disease in patients with hypopituitarism support the possibility that altered endocrine function influences disease susceptibility. This paper catalogues the evidence for immunomodulatory consequences of predicted endocrine changes in Lady Windermere syndrome, with emphasis on the immune response to NTM. Collectively, the data warrant further exploration of an endocrine link to disease susceptibility in Lady Windermere syndrome.

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Effect of Growth Hormone Treatment on Lymphocyte Functions in Old Male Rats

Cited by 13 publications

References 89 publications

Cardiological aging in SAM model: effect of chronic treatment with growth hormone

Cardiological aging in SAM model: effect of chronic treatment with growth hormone

Assessment of immune function after short-term administration of recombinant human growth hormone in healthy young males

Exploring immunomodulation by endocrine changes in Lady Windermere syndrome

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