1974
DOI: 10.1016/0014-5793(74)81111-7
|View full text |Cite
|
Sign up to set email alerts
|

Effect of H1 protein on in vitro ribosomal RNA synthesis

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
6
0

Year Published

1974
1974
1990
1990

Publication Types

Select...
5
2

Relationship

1
6

Authors

Journals

citations
Cited by 7 publications
(6 citation statements)
references
References 11 publications
0
6
0
Order By: Relevance
“…ia coli that stimulate RNA synthesis in vitro have been characterized (1)(2)(3)(4)(5)(6)(7)(8). The heat-stable protein, HI (1,2), was shown to enhance X-lac DNA transcription by E. colt RNA polymerase (3) while causing reduction of ribosomal RNA synthesis in an E. coli DNA-dependent system (4).…”
mentioning
confidence: 99%
“…ia coli that stimulate RNA synthesis in vitro have been characterized (1)(2)(3)(4)(5)(6)(7)(8). The heat-stable protein, HI (1,2), was shown to enhance X-lac DNA transcription by E. colt RNA polymerase (3) while causing reduction of ribosomal RNA synthesis in an E. coli DNA-dependent system (4).…”
mentioning
confidence: 99%
“…Thus it appears likely that both glycerol and dimethylsulphoxide could affect transcription by lowering the melting temperature of DNA in the promoter region. Consistent with this conlusion is the observation that HI protein, which preferentially increases the T, of (G + C)-rich DNA while destabilising (A + T)-rich DNA (Cukier-Kahn and Geiss, personal communication) stimulates RNA synthesis from (A + T)-rich T4 promoters [25] yet increases the transition temperature between the open and closed forms of the rRNA promoters [9]. Nevertheless the disruption of the water envelope around the DNA, which results in the lowering of T, by glycerol and dimethylsulphoxide, could also affect the diameter of the double helix (see for example [26]).…”
Section: Discussionmentioning
confidence: 77%
“…The transition temperature between these two states can be altered by molecules which interact directly with the template. It is, for example, raised by a DNA-binding protein, H I protein [9].In this paper I show that two rather dissimilar compounds, glycerol and dimethylsulphoxide, have very similar effects on transcription in vitro. At low concentrations (up to 20 "/, v/v) both stimulate total RNA synthesis, the extent of stimulation depending on the type of DNA template.…”
mentioning
confidence: 80%
See 1 more Smart Citation
“…Accordingly, our data indicate, for instance, that H1 would greatly favor the "opening" of promoters from "catabolite-sensitive" genes, have only a modest effect on X-specific promoters and have virtually no effect on trp promoters. Moreover, independent observations (15) show that, at temperatures below 42°, E. coli ribosomal promoters are made less accessible to RNA polymerase in vitro when H1 is present. These "differential" effects could be imputed to the fact that H1 "stabilizes" certain DNA regions from the template (particularly in the vicinity of the promoter) while inducing local melting of others.…”
Section: Resultsmentioning
confidence: 99%