Effect of H1 antihistamines in a model of mesenteric ischaemia/reperfusion

Pheniramines and Oxidative Burst of Blood Phagocytes During Ischaemia/Reperfusion

“…Indeed, as compared to sham-operated rats, mesenteric I/R resulted in increased CL of WB ( Fig.2) after reperfusion and PA, CPA and BPA, adminstered i. p., decreased CL significantly after I/R. Neutrophils accumulated and activated during mesenteric ischaemia represent an important source of blood ROM detected by CL [2]. Oxidative burst in endothelial hypoxia and reoxygenation stimulated MAP kinase signalling and kinase-dependent neutrophil recruitment, and cellular signalling is suppressed by ROM scavengers, supporting their use as therapeutic agents in I/R [8].…”

“…After thiopental anaesthesia (50 mg/kg i. p.) to male Wistar rats (200 -280 g, from Dobra Voda breeding station), ischaemia (60 min) was induced by ligation of the superior mesenteric artery; this was followed by 30 min reperfusion after removal of ligature [2]. Blood samples (10ml) were obtained from tail vein at end of reperfusion period.…”

“…Neutrophils play a crucial role in development of reperfusion injury resulting in increased generation of reactive oxygen metabolites (ROM), as demonstrated by increased chemiluminescence (CL) of whole blood during mesenteric ischaemia/reperfusion (I/R) injury in rats [1,2]. Tissue injury due to I/R is accompanied by an inflammatory reaction, activation of ROM and contribution of histaminergic system [3].…”

Assessment of Ischemic Vascular Damage

“…Previous experiments showed that the maximal chemiluminescence increase occurred in the ileum in the course of early postischemic period. Results following that time point (not quite anticipated) need further study, because an important chemiluminescence difference was found between the effects of the compounds tested in vivo versus in vitro: while the compounds were tested effective in vitro, depending on the concentration used, and thus inhibited the chemiluminescence response to I/R, in vivo these compounds further increased intestinal chemiluminescence values (Nosál'ová et al, 2008). These find-ings indicate that in vivo, in addition to receptor involvement and ROS production, other mechanisms, probably non-receptordependent, could play a role.…”

Effect of pheniramine maleate on reperfusion injury in brain tissue

“…Activation of NF-kB is stimulated by histamine and inhibited by antihistaminics [ 3 , 9 , 10 ]. As with the other H 1 -antihistaminics, Ph shows its effect on I/R injury by inhibiting release of some specific mediators of inflammation, which is a significant factor in I/R pathophysiology [ 3 , 11 – 13 ].…”