Effect of H1-antihistamines on the oxidative burst of rat phagocytes

Kráľová, Jana; Čı́ž, Milan; Nosáľ, R; Drábiková, Katarı́na; Lojek, Antonı́n

doi:10.1007/s00011-005-0020-6

Cited by 7 publications

(8 citation statements)

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“…The volume of recently published evidence claims that H 1 -antihistamines play a very important role in the regulation of phagocyte-derived ROS production [7,13,14,21] as well as the regulation of myeloperoxidase activity [22]. But, the effects of H 1 -antihistamines on NO production remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…Detection of nitrite concentrations and iNOS protein expression are considered to be reliable methods for verifying the influence of drugs on the NO production by cells at various levels [12]. Whereas the effect of H 1 -antihistamines on the production of ROS is relatively well described [13,14], the information about the influence of these drugs on RNS generation is still insufficient. Therefore, the main aim of the present study was to investigate the effects of H 1 -antihistamines on NO production by phagocytes.…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

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The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity

Kráľová¹,

Račková²,

Pekarová³

et al. 2009

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity

Kráľová¹,

Račková²,

Pekarová³

et al. 2009

International Immunopharmacology

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“…But chronic inflammation accompanied by a rise in oxidative stress with an overproduction of reactive oxygen and nitrogen species could contribute to the pathogenesis of many inflammatory diseases, such as bronchitis or rheumatoid arthritis. Whereas the effect of dithiaden on the production of reactive oxygen species is relatively well described (Kralova et al ., 2006; Nosal et al ., 2002; Nosal et al ., 2006), the information about the role of dithiaden on reactive nitrogen species generation is insufficient. Therefore, the present study investigated the effects of dithiaden on nitric oxide (NO) production by murine macrophages like cells RAW 264.7 stimulated by lipopolysaccharide and also tries to detect the direct scavenging properties of dithiaden against NO in a cell free system.…”

Section: Introductionmentioning

confidence: 99%

The effects of dithiaden on nitric oxide production by RAW 264.7 cells

Kráľová¹,

Pekarová²,

Drábiková³

et al. 2008

Interdisciplinary Toxicology

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As reported in our previous studies, dithiaden (an antagonist of histamine H1-receptor, used clinically as an anti-allergic or anti-emetic drug) in a concentration range of 5×10−5–10−4 M decreased the production of reactive oxygen species by phagocytes. In this study we investigated the influence of dithiaden on nitric oxide (NO) production by LPS-stimulated macrophages.The cell viability in the presence of 10−4–5×10−5 M dithiaden was evaluated by an ATP-test. RAW 264.7 cells (2.5×106/well) were preincubated with dithiaden for 60 mins and subsequently stimulated with 0.1 µg/ml of bacterial lipopolysaccharide. After incubating for 24 hours the NO production was determined spectrophotometrically using Griess reaction as a concentration of nitrites (the end product of NO metabolism) accumulated in the cell supernatants. The expression of inducible nitric oxide synthase (iNOS) in cell-lysates was evaluated using Western blot analysis. Scavenging properties of dithiaden against NO were evaluated amperometrically.Our data demonstrate that dithiaden in the concentration of 5×10−5 M (approved by ATP test as non toxic) caused a significant decrease in the accumulation of nitrites, and in addition, this decline was followed by a marked reduction of iNOS protein expression. Amperometrical analysis did not show any scavenging properties of dithiaden against NO.From this data it can be suggested that the inhibition effect of dithiaden on macrophage NO production is caused exclusively by the suppression of iNOS protein expression.

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“…The chemiluminescence response of these neutrophils was inhibited by 10 -4 and 10 -3 M of the histamine H1 receptor antagonists, promethazine, pyrilamine and diphenhydramine, by calcium and magnesium depletion, and by 10 nmol of staurosporine. The chemiluminescence signal of neutrophils was also partially inhibited by pertussis toxin (4 mg·mL neutrophils to produce ROS via histamine H1 receptors and the NADPH oxidase pathway.In another study (Kralova et al, 2006), the inhibitory effects of dithiaden (a first-generation H1-antihistamine, concentration range 10 -6 -5 ¥ 10 -4 M) on the production of ROS by rat neutrophils were compared with those of four secondgeneration H1-antihistamines (10 -6 -5 ¥ 10 -4 M) -loratadine, acrivastine, astemizole and ketotifen-fumarate. In general, the second-generation H1 antihistamines exerted different effects on the chemiluminescence response of rat neutrophils depending on their chemical structure, selectivity and affinity for histamine H1 receptors.…”

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confidence: 99%

Modulation of neutrophil oxidative burst via histamine receptors

Čı́ž

Lojek

2013

British J Pharmacology

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Histamine has the ability to influence the activity of immune cells including neutrophils and plays a pivotal role in inflammatory processes, which are a complex network of cellular and humoral events. One of the main functions manifested by activated neutrophils is oxidative burst, which is linked to the production of reactive oxygen species; therefore, the effects of histamine receptor agonists and antagonists on the oxidative burst of neutrophils is reviewed. A role for the well-characterized histamine H1 and H2 receptors in this process is discussed and compared to that of the recently discovered H4 receptor. LINKED ARTICLESThis article is part of a themed issue on Histamine Pharmacology Update. To view the other articles in this issue visit http://dx.doi.org/10.1111/bph.2013.170.issue-1 AbbreviationsCaI, calcium ionophore A23187; N-fMLP, N-formyl-methionyl-leucyl-phenylalanine; OZP, opsonized zymosan particle; PMA, phorbol myristate acetate; ROS, reactive oxygen species; TLR, toll-like receptor Oxidative burst of neutrophilsNeutrophils are the most abundant type of white blood cells, comprising about 50-70% of all leukocytes. One of the most important defence mechanisms of neutrophils is associated with their ability to mediate a strong oxidative burst through the formation of reactive oxygen species (ROS). While oxidative burst is important for the elimination of invading microorganisms, the overproduction of ROS or the impairment of endogenous antioxidant defences may result in detrimental effects on the host's own cells and tissues (Freitas et al., 2009). Neutrophil oxidative burst is accompanied by the production of NADPH oxidase, which reduces oxygen to a superoxide anion radical. It is generally assumed that the NADPH oxidase is activated exclusively in the plasma membrane. However, in neutrophils, this assumption does not fit with the subcellular localization of the membrane components of the NADPH oxidase, which are stored in the granular compartments, and it has become increasingly evident that oxidants are also produced in an intracellular compartment, identified as specific granules. Myeloperoxidase is stored in another subset of granules, the azurophil granules, and participates in the processing of the ROS. In fact, it has been suggested that neutrophil activation is accompanied by the fusion of azurophil with specific granules, allowing these peroxidase-dependent reactions to take place (Karlsson and Dahlgren, 2002). PKC-d is required for full production of NADPH oxidase and activation of the respiratory burst. Neutrophils also express PKC-a and b, which may be involved in adhesion, degranulation and phagocytosis, but the evidence for this is not yet conclusive (Bertram and Ley, 2011). Although the complex mechanisms that coordinate the membrane traffic, oxidative burst and release of granule contents required for the microbicidal activities of neutrophils are not completely understood, it is evident that they are unique and differ from those in macrophages (Nordenfelt and Tapper, 2011). Neutr...

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Effect of H1-antihistamines on the oxidative burst of rat phagocytes

Cited by 7 publications

References 7 publications

The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity

The effects of H1-antihistamines on the nitric oxide production by RAW 264.7 cells with respect to their lipophilicity

The effects of dithiaden on nitric oxide production by RAW 264.7 cells

Modulation of neutrophil oxidative burst via histamine receptors

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