Effect of halogenated indoles on biofilm formation, virulence, and root surface colonization by Agrobacterium tumefaciens

Ahmed, Bilal; Jailani, Afreen; Lee, Jin-Hyung

doi:10.1016/j.chemosphere.2022.133603

Cited by 19 publications

(19 citation statements)

References 74 publications

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“…These indoles have been studied for their antimicrobial and antibiofilm activities on diverse microorganisms. It has been reported that halogenated indoles effectively reduced biofilm and hyphal formation by fungi like C. albicans and eradicated B. cinerea fruit infection ( Manoharan et al, 2018 ; Raorane et al, 2022 ), reduced biofilm formation, virulence, and root surface colonization by Agrobacterium tumefaciens ( Ahmed et al, 2022 ). In addition, they effectively killed metabolically dormant persister cells in S. aureus and P. aeruginosa biofilms ( Manoharan et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli

Boya

Lee

2022

Front. Microbiol.

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Uropathogenic Escherichia coli (UPEC) is a nosocomial pathogen associated with urinary tract infections and expresses several virulence factors that cause recurring infections and cystitis of the bladder, which can lead to pyelonephritis. UPEC uses different types of extracellular appendages like fimbriae and pili that aid colonization and adherence to bladder epithelium and can form persistent biofilm-like bacterial communities that aid its survival after the deployment of host immune responses. We investigated the antibiofilm, antimicrobial, and antivirulence properties of three indole derivatives namely, 4-chloroindole, 5-chloroindole, and 5-chloro 2-methyl indole. All the three chloroindoles had MICs of 75 μg/ml and inhibited biofilm formation by an average of 67% at 20 μg/ml. In addition, they inhibited swarming and swimming motilities, which are essential for dissemination from bacterial communities and colonization, reduced cell surface hydrophobicity, and inhibited indole production and curli formation. Gene expression analysis showed all three chloroindoles significantly downregulated the expressions of virulence genes associated with adhesion, stress regulation, and toxin production. A 3D-QSAR analysis revealed substitutions at the fourth and fifth positions of the indole moiety favored antimicrobial activity. Furthermore, these chloroindoles potently inhibited biofilm formation in other nosocomial pathogens and polymicrobial consortia.

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Section: Discussionmentioning

confidence: 99%

Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli

Boya

Lee

2022

Front. Microbiol.

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“…A. tumefaciens can sense chemical signals released from plant wounds and uses its swimming motility to reach these sites [ 12 ]. Swarming and twitching motilities have not been reported for this genus, and thus, swimming motility is believed to be the only means of locomotion as reported [ 12 ] and ourselves [ 30 ]. In a previous study, tannic acid inhibited the swimming motility of C. violaceum (a quorum-sensing-positive bacteria) at concentrations of ≥30 µg/mL [ 50 ], and in the present study, tannic acid at 25 µg/mL reduced the swimming motility of A. tumefaciens ( Figure 5 D,E).…”

Section: Discussionmentioning

confidence: 95%

“…A. tumefaciens resides in the top layer of soil and can adhere to biotic and abiotic surfaces [ 3 , 30 ]. The transition from sessile to surface-attached A. tumefaciens is determined by adhesins and flagellar motility [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Before pouring into Petri plates, the agar was thoroughly mixed with different concentrations of tannic acid. Aliquots (1 µL) of 24 h cultures were placed at the centers of agar plates and incubated at 30 °C for 72 h. Swimming diameters were recorded at every 48 h and 72 h during incubation [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Time-dependent growth of A. tumefaciens in the presence of tannic acid was used to investigate the ability of tannic acid to inhibit planktonic cell growth following our earlier described method [30]. After culturing for 24 h, A. tumefaciens was diluted with LB at 1:100, and tannic acid was added to final concentrations of 10,20,25,50,75,100,150,200, or 400 µg/mL.…”

Section: Growth Assessments Of Tannic Acid Treated a Tumefaciensmentioning

confidence: 99%

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Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid

et al. 2022

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Agrobacterium tumefaciens underlies the pathogenesis of crown gall disease and is characterized by tumor-like gall formation on the stems and roots of a wide variety of economically important plant species. The bacterium initiates infection by colonizing and forming biofilms on plant surfaces, and thus, novel compounds are required to prevent its growth and biofilm formation. In this study, we investigated the ability of tannic acid, which is ubiquitously present in woody plants, to specifically inhibit the growth and biofilm formation of A. tumefaciens. Tannic acid showed antibacterial activity and significantly reduced the biofilm formation on polystyrene and on the roots of Raphanus sativus as determined by 3D bright-field and scanning electron microscopy (SEM) images. Furthermore, tannic acid dose-dependently reduced the virulence features of A. tumefaciens, which are swimming motility, exopolysaccharide production, protease production, and cell surface hydrophobicity. Transcriptional analysis of cells (Abs600 nm = 1.0) incubated with tannic acid for 24 h at 30 °C showed tannic acid most significantly downregulated the exoR gene, which is required for adhesion to surfaces. Tannic acid at 100 or 200 µg/mL limited the iron supply to A. tumefaciens and similarly reduced the biofilm formation to that performed by 0.1 mM EDTA. Notably, tannic acid did not significantly affect R. sativus germination even at 400 µg/mL. The findings of this study suggest that tannic acid has the potential to prevent growth and biofilm formation by A. tumefaciens and thus infections resulting from A. tumefaciens colonization.

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Inhibitory effects of bromoindoles on Escherichia coli O157:H7 biofilms

Jeon,

Boya,

Kim

et al. 2024

Biotechnol Bioproc E

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Effect of halogenated indoles on biofilm formation, virulence, and root surface colonization by Agrobacterium tumefaciens

Cited by 19 publications

References 74 publications

Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli

Antibiofilm and Antimicrobial Activities of Chloroindoles Against Uropathogenic Escherichia coli

Inhibition of Agrobacterium tumefaciens Growth and Biofilm Formation by Tannic Acid

Inhibitory effects of bromoindoles on Escherichia coli O157:H7 biofilms

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