Effect of haloperidol on the numeric density of neurons and nuclear height in the rat hippocampus: A stereological and histopathological study

Ünal, Bünyami; Mn, Ozbek; Aydın,; Bulucu, Z.; Vuraler, Özgen; Odacı, Ersan; Şahin, Bünyamin; Kaplan, Süleyman

doi:10.1002/nrc.10100

Cited by 22 publications

(18 citation statements)

References 26 publications

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“…al., 2005). An unbiased counting frame was placed on the reference and the look-up sections on the screen of the PC to perform the counting according to the disector counting method (Unal et al, 2004).…”

Section: Estimation Of Glomerular Numbermentioning

confidence: 99%

Effects of the melatonin on the kidney of high fat diet fed obese rats: A stereological and histological approach

Yurt¹,

KAYHAN²,

Altunkaynak³

et al. 2013

jecm

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Article HistoryReceived 25/ 02 /2012 Accepted 22/ 05 /2013Obesity is associated with multiple conditions that are known to compromise renal function, including hypertension, diabetes, hyperuricemia, and the metabolic syndrome that can independently have a detrimental effect on renal function. The aim of this study was to examine effects of fatty diet induced obesity and melatonin on kidney in female rats by histological and quantative methods. For this aim, 24 rats were randomly divided into 4 groups including Non-obese control (NC group), Obese control (OC group), Nonobese Melatonin (NOM group) and Obese-Melatonin (OM group) groups. In the obese groups, rats, were fed with high fat diet (40% of calories from fat) for 15 weeks. The rats of the non-obese groups were fed with standard, commercial rat diet during the same period. At the end of the 15 th week, rats in the melatonin groups started to received daily injections (i.p.) 10 mg/kg melatonin for 6 weeks. At the end of the experiment, a serial sections of kidneys were stained with Hematoxylen-eosin. Glomerular number (Ng) is considered a major determinant of renal function and outcome. Unbiased stereological methods have been used to estimate of Ng. Also histopathological analysis was made on the same sections. Stereological examination of the kidneys showed differences in terms of total kidney volume, volume of cortex, medulla and numerical density of glomeruli among the groups. Light microscopic investigation showed a dilatation in blood vessels and Bowman's space, mononuclear cell infiltration, degeneration in nephrons, including glomerulosclerosis and tubular defects, and an increase in the connective tissue in the kidneys in the OC group. But, both stereological deficits and histological damages detected in OC group recovered in OM group after melatonin treatment. We suggested that a fatty diet is responsible for the rats' obesity and may lead to renal deformities as a result of histopathological changes such as vessel dilatation, tubular defects, inflammation and connective tissue enlargement of the kidney. Also melatonin treatment after obesity may contribute structural and functional healing.

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Section: Estimation Of Glomerular Numbermentioning

confidence: 99%

Effects of the melatonin on the kidney of high fat diet fed obese rats: A stereological and histological approach

Yurt¹,

KAYHAN²,

Altunkaynak³

et al. 2013

jecm

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“…[21,22] Stereological quantifications and histopathological examinations were performed on the light microscopic sections. The results of both approaches confirmed each other.…”

Section: Discussionmentioning

confidence: 99%

“…This number is in an acceptable range for stereological analysis. [22,23] Two consecutive sections were mounted on each slide. Photographs of adjacent sections were taken with a digital camera at a magnification of 400×.…”

Section: Numerical Density and Total Number Of Glomerulimentioning

confidence: 99%

“…Photographs of adjacent sections were taken with a digital camera at a magnification of 400×. An unbiased counting frame [22] was placed on the reference and the look-up sections on the screen of the PC, to perform the counting according to the dissector counting method. The bottom and the left-hand edges of the counting frame were considered to be the forbidden (exclusion) lines together with the extension lines.…”

Section: Numerical Density and Total Number Of Glomerulimentioning

confidence: 99%

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Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

et al. 2009

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Haloperidol, a typical antipsychotic, is the most commonly prescribed medication for the treatment of mental health problems such as agitation and psychosis. We attempted to determine the effects of haloperidol treatment on the kidneys of female rats. In addition, we aimed to estimate the numerical density, total number, and height of renal glomeruli and the volume and volumetric fractions of the cortex, medulla, and whole kidneys, and tried to determine whether there was a change in these stereological parameters depending on haloperidol treatment. Both the qualitative and quantitative histological features of the kidney samples were analyzed with conventional histopathological and modern stereological methods at the light microscopic level. The total number of glomeruli and numerical density of glomerulus in the haloperidoltreated groups was not changed by increasing the dose in comparison to the control group. The mean height of the glomerulus significantly increased, especially in low-dose groups. In the haloperidol-treated groups, the volumetric fractions of the cortex to the whole kidney of the rats were significantly decreased by increasing the dose. The volumetric fractions of the medulla to the whole kidney of the rats were increased significantly in parallel by the given dose. In addition, we present quantitative findings showing that haloperidol is associated with many alterations in rat kidneys. It was shown that haloperidol may lead to undesirable changes in the kidney after chronic treatment with especially high doses.

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“…This number is in an acceptable range for stereological analysis. 20,21 Two consecutive sections were mounted on each slide. Photographs of adjacent sections were taken with a digital camera at a magnification of 400.…”

Section: Numerical Density and Total Number Of Glomerulimentioning

confidence: 99%

Inhalation of mercury vapor can cause the toxic effects on rat kidney

Akgül

Altunkaynak

et al. 2016

Renal Failure

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Dental amalgam has been used in dentistry as a filling material. The filler comprises mercury (Hg). It is considered one of the most important and widespread environmental pollutants, which poses a serious potential threat for the humans and animals. However, mercury deposition affects the nervous, cardiovascular, pulmonary, gastrointestinal, and especially renal systems. In most animals' species and humans, the kidney is one of the main sites of deposition of mercury and target organ for its toxicity. In this study, the effects of mercury intake on kidney in rats were searched. For the this purpose; we used 24 adult female Wistar albino rats (200 g in weight) obtained from Experimental Research and Application Center of Atatü rk University with ethical approval. Besides, they were placed into a specially designed glass cage. Along this experiment for 45 days, subjects were exposed to (1 mg/m 3 /day) mercury vapor. However, no application was used for the control subjects. At the end of the experiment, kidney samples were obtained from all subjects and processed for routine light microscopic level and stereological aspect were assessed. Finally, according to our results, mercury affects the histological features of the kidney. That means, the severe effects of mercury has been shown using stereological approach, which is one of the ideal quantitative methods in the current literature. In this study, it was detected that chronic exposure to mercury vapor may lead to renal damage and diseases in an experimental rat model. ARTICLE HISTORY

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Effect of haloperidol on the numeric density of neurons and nuclear height in the rat hippocampus: A stereological and histopathological study

Cited by 22 publications

References 26 publications

Effects of the melatonin on the kidney of high fat diet fed obese rats: A stereological and histological approach

Effects of the melatonin on the kidney of high fat diet fed obese rats: A stereological and histological approach

Does Haloperidol Have Side Effects on Histological and Stereological Structure of the Rat Kidneys?

Inhalation of mercury vapor can cause the toxic effects on rat kidney

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