Effect of Halothane and Enflurane Anaesthesia on the Level of Reduced Glutathione in Human Red Blood Cells

Agar, N.S.; Lewis, G. B. H.

doi:10.1177/0310057x8000800313

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…Among blood cells, GSH has been most studied in erythrocytes (1)(2)(3)(4), and it has been investigated to a lesser extent in leukocytes of mice (5) and humans (6,7). The lack of studies of leukocytes can in part be ascribed to the relative difficulty involved in the effective separation of the different leukocyte types, the relative paucity of monocytes among the more prevalent neutrophils and lymphocytes, and the general lack of understanding of the physiologic role of GSH in these cells.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous measurement of neutrophil, lymphocyte, and monocyte glutathione by flow cytometry

Scott

Collins

Matin

et al. 1990

Clinical Laboratory Analysis

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A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols closely reflected the GSH content, as measured by a specific glutathione reductase assay. Fluorescence of individual cell types could be measured after delineating those cells by their light-scatter characteristics, utilizing dual-angle light scatter for discrimination. By this means, GSH contents of 12.5 +/- 2.0 nmol/10(7) neutrophils, 14.5 +/- 2.7 nmol/10(7) monocytes, and 5.0 +/- 1.0 nmol/10(7) lymphocytes were found. The results obtained for neutrophils with the flow cytometer were virtually identical with those obtained with chemical assay in purified samples of neutrophils, indicating the validity of the flow cytometric method.

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