Effect of harmaline on firing pattern of rat cerebellar Purkinje cells in ontogenesis

Karelina, T. V.

doi:10.1134/s0022093008010118

Cited by 1 publication

(1 citation statement)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Harmaline provokes high frequency oscillations of IO neurons leading to CF-PC circuitry stimulation which forces PCs to generate complex spikes with higher firing frequency (19). In agreement with the previous studies (26, 27), in our experiments IP injections of 15 mg/kg harmaline resulted in significant increase of complex spike firing frequency and the disappearance of simple spikes from the PC discharge in both WT (Fig. 3 A, B ) and SCA2-58Q (Fig.…”

Section: Resultssupporting

confidence: 94%

In Vivo Analysis of the Climbing Fiber-Purkinje Cell Circuit in SCA2-58Q Transgenic Mouse Model

2018

View full text Add to dashboard Cite

Cerebellar Purkinje cells (PCs) and cerebellar pathways are primarily affected in many autosomal dominant cerebellar ataxias. PCs generate complex spikes (CS) in vivo when activated by climbing fiber (CF) which rise from the inferior olive. In this study, we investigated the functional state of the CF-PC circuitry in the transgenic mouse model of spinocerebellar ataxia type 2 (SCA2), a polyglutamine neurodegenerative genetic disease. In our experiments, we used an extracellular single-unit recording method to compare the PC activity pattern and the CS shape in age-matched wild-type mice and SCA2-58Q transgenic mice. We discovered no alterations in the CS properties of PCs in aging SCA2 mice. To examine the integrity of the olivocerebellar pathway, we applied harmaline, an alkaloid that acts directly on the inferior olive neurons. The pharmacological stimulation of olivocerebellar circuit by harmaline uncovered disturbances in SCA2-58Q PC activity pattern and in the complex spike shape when compared with age-matched wild-type cells. The abnormalities in the CF-PC circuitry were aggravated with age. We propose that alterations in CF-PC circuitry represent one of potential causes of ataxic symptoms in SCA2 and in other SCAs.

show abstract

Section: Resultssupporting

confidence: 94%