Effect of harvest time on the compositional changes in essential oils, cannabinoids, and waxes of hemp (
            <i>Cannabis sativa</i>
            L.)

Noppawan, Pakin; Bainier, Camille; Lanot, Alexandra; McQueen‐Mason, Simon J.; Supanchaiyamat, Nontipa; Attard, Thomas M.; Hunt, Andrew J.

doi:10.1098/rsos.211699

Cited by 8 publications

(2 citation statements)

References 57 publications

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“…It is an economically important crop cultivated worldwide for industrial, cosmetic, food, and therapeutic uses (Murovec et al 2022;De Prato et al 2023). The global sales market for Cannabis is expected to reach US$102 billion by 2026, and the global market demand for cannabinoids is expected to reach $9.69 billion by 2025 (Noppawan et al 2022;Robertson et al 2023). According to the University of Thai Chamber of Commerce report, the Thai Cannabis market is anticipated to reach $1.2 billion by 2025 (Suttinee 2022).…”

Section: Introductionmentioning

confidence: 99%

Effect of carbon source and elicitors on biomass production and accumulation of friedelin and epifriedelanol in hairy roots of hemp (Cannabis sativa L.)

Mahendran,

Vimolmangkang

2024

Plant Cell Tiss Organ Cult

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Friedelin and epifriedelanol are pentacyclic triterpenoids that preferentially accumulate in the roots of hemp (Cannabis sativa L.) and are valued for their antidiabetic, hypolipidemic, antioxidant, liver protective, anti-ulcer, anti-in ammatory, antimicrobial, anticancer, and antisenescence properties. The aim of the present study was to investigate the in uence of media, carbon sources, and elicitation on the production of C. sativa hairy root biomass and these metabolites. The MS liquid medium promoted the highest fresh weight (9.45 ± 0.00 g/100 mL ask) biomass production in hairy root cultures after 28 days.The highest levels of epifriedelanol (3.79-fold) and friedelin (3.25-fold) were found at the end of the exponential phase. The presence of 3% sucrose provided the highest accumulation of epifriedelanol (0.930 ± 0.013 mg/g DW) and friedelin (0.574 ± 0.024 mg/g DW) in the roots. The effects of methyl jasmonate (MJ) and salicylic acid (SA) on the enhancement of friedelin and epifriedelanol in C. sativa hairy root cultures were investigated. Between the two elicitors, SA showed the highest production of epifriedelanol (up to 5.018 ± 0.35 mg/g DW) and friedelin up to 1.56 ± 0.34 mg/g DW in 28-day-old stationary phase hairy roots. These represented 5.22-and 2.88-fold increase over the control (0.96 ± 0.01 mg/g DW and 0.54 ± 0.03 mg/g DW) after 96 h of treatment, respectively. The maximum accumulations of epifriedelanol (3.59 ± 0.12 mg/g DW) and friedelin (1.31 ± 0.01 mg/g DW) were observed in the treatment with MJ (100 µM) after 24 h of exposure and were 3.73-and 2.44-fold higher than the control, respectively. These ndings suggest that elicitation is an effective technique for enhancing the yields of these valuable bioactive pentacyclic triterpenoids in C. sativa hairy root cultures in a relatively short period of time.

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Section: Introductionmentioning

confidence: 99%

Effect of carbon source and elicitors on biomass production and accumulation of friedelin and epifriedelanol in hairy roots of hemp (Cannabis sativa L.)

Mahendran,

Vimolmangkang

2024

Plant Cell Tiss Organ Cult

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“…Hemp leaves are widely used industrially as livestock feed, animal bedding textile material, construction and paper components, fuel and biochar substrate [2]. Due to their high mass proportion relative to the inflorescences, despite their lower substance content, they can be a source of valuable phytochemicals such as essential oils or cannabinoids [3]. The extraction of essential oils in the case of raw herbal materials is performed usually from dried material.…”

Section: Introductionmentioning

confidence: 99%

Effect of Drying Methods on Chemical and Sensory Properties of Cannabis sativa Leaves

Kwaśnica,

Pachura,

Carbonell-Barrachina

et al. 2023

Molecules

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Hemp is used as a source of fiber, oil and bioactive substances including volatile and cannabinoid-containing substances. This paper presents, for the first time, results on the evaluation of drying methods (convective, vacuum–microwave and combined convective pre-drying and vacuum–microwave finishing drying) of hemp leaves on the qualitative and quantitative changes in secondary metabolites, including essential oils, cannabinoids and sterols. A ranking and descriptive test of hemp leaves was also performed. Drying kinetics was presented using three models, including logarithmic, Midilli and modified Page. The SPME-Arrow technique was used to determine 41 volatile compounds, of which caryophyllene, β-myrcene and α-humulene were dominant in dried and fresh leaves. Regarding the essential oils obtained, 64 were identified, with caryophyllene, humulene epoxide II and limonene being the dominant ones. For preserving the highest amount of oils, the best method was the convective pre-drying followed by vacuum–microwave finishing drying (CD60-VMD) combined method, where the retention of volatile compounds was 36.08%, whereas the CD70 and 240-VMD methods resulted in the highest loss of 83%. The predominant cannabinoids in fresh hemp leaves were CBDA 6.05 and CBD 2.19 mg g−1. Drying caused no change in the cannabinoid profile of the plant material. β-Sitosterol, campesterol and lupeol were dominant in the phytosterol and triterpene fractions. No changes in either quality or quantity were observed in any of the variants found.

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Partially substituting alfalfa hay with hemp forage promotes the health and well‐being of goats via altering ruminal and plasma metabolites and metabolic pathways

Ran,

Luo,

Yue

et al. 2024

Animal Research and One Health

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Hemp forage (HF) seems a suitable forage for ruminants for its high nutritional value and rich phytochemicals that exert health and growth‐promoting activities. We investigated the effects of hemp‐related phytochemicals on rumen and plasma metabolism using metabolome when partially substituting alfalfa hay with HF in goat diets. Numbers of differential metabolites linearly increased with increasing HF substituting rate, approximately 50% of which were phytochemicals. Metabolic pathway enrichment analysis showed that the inclusion of HF greatly promoted steroid hormone biosynthesis, one carbon pool by folate, and retinol metabolism pathways in both rumen and plasma, which are beneficial for promoting animal health and well‐being and enhancing the quality of animal products. Some phytochemicals showed inhibitory activities on the growth of certain ruminal bacteria; meanwhile, the detected intermediate metabolites indicated degradation of the phytochemicals by ruminal microbes. These phytochemicals work individually and synergistically to alter ruminal and plasma metabolic pathways, thus exerting benefits in promoting the health and well‐being of animals.

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Effect of harvest time on the compositional changes in essential oils, cannabinoids, and waxes of hemp ( Cannabis sativa L.)

Cited by 8 publications

References 57 publications

Effect of carbon source and elicitors on biomass production and accumulation of friedelin and epifriedelanol in hairy roots of hemp (Cannabis sativa L.)

Effect of carbon source and elicitors on biomass production and accumulation of friedelin and epifriedelanol in hairy roots of hemp (Cannabis sativa L.)

Effect of Drying Methods on Chemical and Sensory Properties of Cannabis sativa Leaves

Partially substituting alfalfa hay with hemp forage promotes the health and well‐being of goats via altering ruminal and plasma metabolites and metabolic pathways

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