The present investigation entitled, Response of carnation varieties (Dianthus caryophyllus) to mycorrhizal fungi under naturally ventilated polyhouse conditions in Prayagraj was conducted at horticulture research field, Department of Horticulture, Naini Agricultural Institute, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U.P, during the winter season (2022). The experiment was laid out in Factorial Randomized block design (FRBD) with 3 varieties and 5 VAM treatments in 15 treatments and each replicated thrice. From the present investigation, Variety-3 (Kiro-yellow) treated with 10g VAM per plant performed better for plant height (55.44cm), primary branches (7.22), bud diameter (21.72mm), and yield per plant (5.74). In terms of flower diameter (6.16cm) and vase life (13.33 days) Variety-1 (Cervantes) treated with 10g VAM performed better. In terms of first bud initiation Variety-2 (Baltico-White) treated with 5g VAM per plant showed better performance (59.11 days).