Effect of He-Ne laser radiation on healing of osteochondral defect in rabbit: A histological study

Abstract

Abstract-This study examined the influence of low-level laser therapy (LLLT) on the healing of a large osteochondral defect in rabbits. An osteochondral defect with 5 mm diameter was surgically induced in the right femoral patellar groove of 48 adult male rabbits. They were divided into a control and an experimental group. The rabbits were treated at 2, 4, 8, and 16 weeks after surgery, with six rabbits in each study period being tested at each biweekly period. The experimental group received LLLT with a helium-neon (He-Ne) laser (632.8 nm, 10 mW) of 148.4 J/cm 2 three times a week, and the control group received placebo LLLT with equipment switched off. The defects were examined macroscopically and microscopically. The results of the histological examination 2 weeks after surgery showed that the osteochondral healing of the control group was significantly accelerated compared with that of the experimental group. However, the osteochondral healing of the experimental group 4 weeks and 16 weeks after surgery showed that healing accelerated significantly compared with that of the control group. The conclusion was that LLLT with an He-Ne laser could not significantly accelerate healing of a large osteochondral defect in rabbits of the experimental group compared with that of the control group throughout the duration of the present study.

Lasers in orthopaedic surgery

“…It was shown that the healing of the lesion was faster at week 2 after laser treatment than for control. A study by Bayat et al 55 examined the infl uence of LILT (using a He-Ne laser) on the healing of a large (5 mm in diameter) osteochondral defect in rabbits. It was shown that laser radiation diminished the rate of healing during the fi rst 2 weeks, and accelerated regeneration over a longer period of observation (4 and 16 weeks).…”

The efficacy of low-level 940 nm laser therapy with different energy intensities on bone healing

“…6 Different laser types with different wavelengths including Helium-Neon (He-Ne) and Diode (gallium-aluminium-arsenide, gallium-arsenide) have been used at different doses and different treatment schedules for LLLT. 7,8 In recent studies, the Ga-Al-As type of diode lasers has been shown to be more effective in biostimulation than He-Ne lasers due to the higher penetration ability into the deep tissues. 9 The dose-dependent…”

The effects of grapeseed extract and low level laser therapy administration on the liver in experimentally fractured mandible

“…Thus, it was also mentioned that by triggering vascularization and inflammatory response, it facilitates bone matrix production as well (30). However, in certain studies, although the positive effect of LLLT on hard and soft tissue recovery was demonstrated, it was observed that the estimated effect on recovery was not seen or the laser had negative effects (31)(32)(33). In our study, we observed that MDA and CAT levels in the liver of the rats in the groups where mandibular defect + laser were applied both for 7 and 21 days were similar to that of the mandibular defect group (P<0.05).Under certain conditions where free radicals are produced extensively such as inflammation or trauma, endogenous antioxidants could be insufficient.…”