Effect of Heat Inactivation of HIV on Specific Serum Proteins and Tumour Markers

An enzyme-linked immunosorbent assay and a Western blot analysis were developed to study the antibody response to Pneumocystis carinii in serum and bronchoalveolar lavage fluid from 27 human immunodeficiency virus 27 (HIV)-infected patients with P. carinii pneumonia (Pcp), 32 patients without Pcp, and 51 HIV-negative controls. Urea was used for the correct dilution of epithelial lining fluid, and albumin was used to evaluate transudation from plasma for the assessment of local production of antibodies to P. carinii. By contrast with those of immunoglobulin G (IgG), IgA responses to P. carinii were increased in serum from HIV-positive patients compared to negative controls. Local production of antibodies to P. carinii, especially IgA, was decreased in patients with Pcp. In a study of 10 patients of each group, IgG and IgA responses to gp116 from P. carinii were lower in patients with Pcp than in other groups. These results suggest that, in addition to alveolar macrophages, local antibodies may play a role in host defense against P. carinii.

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“…These conditions did not affect IgG and IgA activities (9). The samples were aliquoted immediately and frozen at Ϫ80°C until use.…”

Section: Methodsmentioning

confidence: 99%

Decreased Production of Local Immunoglobulin A toPneumocystis cariniiin Bronchoalveolar Lavage Fluid from Human Immunodeficiency Virus-Positive Patients

Jalil¹,

Moja²,

Lambert³

et al. 2000

Infect Immun

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“…Heat inactivation can be done simply in a biological safety cabinet with a waterbath at 60°C, treating samples for 30 min to inactivate corona viruses or for 60 min to inactivate Lassa, Ebola, or Marburg viruses (3 ). Only limited and nonquantitative data are available on the effects of these procedures on tests for common, clinically important analytes (3,4,6 ). We examined the effect of these two heat inactivation procedures as well as the effect of a virus-envelope-destroying detergent on tests used in intensive and emergency care.…”

mentioning

confidence: 99%

Influence of Practicable Virus Inactivation Procedures on Tests for Frequently Measured Analytes in Plasma

et al. 2004

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“…The effect of IgA/IgG on C . rodentium virulence did not depend on complement as heating the supernatants to 56°C for 30 minutes to inactivate complement ( 24 ) did not efface induction of ler expression ( Supplemental Figure 4 ). Finally, we determined whether virulence-inducing IgA necessarily reflected production by mutant mice or could have been acquired from maternal transfer.…”

Section: Resultsmentioning

confidence: 99%

TNFRSF13B polymorphisms counter microbial adaptation to enteric IgA

et al. 2021

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TNFRSF13B encodes the transmembrane activator and CAML interactor (TACI) receptor, which drives plasma cell differentiation. Although TNFRSF13B supports host defense, dominant-negative TNFRSF13B alleles are common in humans and other species and only rarely associate with disease. We reasoned that the high frequency of disruptive TNFRSF13B alleles reflects balancing selection, the loss of function conferring advantage in some settings. Testing that concept, we investigated how a common human dominant-negative variant, TNFRSF13B A181E, imparts resistance to enteric pathogens. Mice engineered to express mono- or biallelic A144E variants of tnrsf13B , corresponding to A181E, exhibited a striking resistance to pathogenicity and transmission of Citrobacter rodentium , a murine pathogen that models enterohemorrhagic Escherichia coli , and resistance was principally owed to natural IgA deficiency in the intestine. In WT mice with gut IgA and in mutant mice reconstituted with enteric IgA obtained from WT mice, IgA induces LEE expression of encoded virulence genes, which confer pathogenicity and transmission. Taken together, our results show that C . rodentium and most likely other enteric organisms appropriated binding of otherwise protective antibodies to signal induction of the virulence program. Additionally, the high prevalence of TNFRSF13B dominant-negative variants reflects balancing selection.

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Effect of Heat Inactivation of HIV on Specific Serum Proteins and Tumour Markers

Cited by 13 publications

References 14 publications

Decreased Production of Local Immunoglobulin A toPneumocystis cariniiin Bronchoalveolar Lavage Fluid from Human Immunodeficiency Virus-Positive Patients

Decreased Production of Local Immunoglobulin A toPneumocystis cariniiin Bronchoalveolar Lavage Fluid from Human Immunodeficiency Virus-Positive Patients

Influence of Practicable Virus Inactivation Procedures on Tests for Frequently Measured Analytes in Plasma

TNFRSF13B polymorphisms counter microbial adaptation to enteric IgA

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