Effect of heat stress-induced production of mitochondrial reactive oxygen species on NADPH oxidase and heme oxygenase-1 mRNA levels in avian muscle cells

Kikusato, Motoi; Yoshida, H.; Furukawa, Kyohei; Toyomizu, Masaaki

doi:10.1016/j.jtherbio.2015.04.005

Cited by 51 publications

(37 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The rapid depletion of the ETC proteins from Complexes I-IV during the heat shock is known to lead to a rapid and drastic drop in ATP levels in the cell. This process is coupled to the release of reactive oxygen species (ROS) (64). These observations are congruent with the notion that hyperthermia promotes the disassembly of the mitochondrial wall, resulting in the release of its content.…”

Section: Dynamic Proteomics Enabled the Profiling Of Cellular Responssupporting

confidence: 67%

A Novel Differential Ion Mobility Device Expands the Depth of Proteome Coverage and the Sensitivity of Multiplex Proteomic Measurements

Pfammatter

Bonneil

McManus

et al. 2018

Molecular & Cellular Proteomics

121

142

View full text Add to dashboard Cite

show abstract

Section: Dynamic Proteomics Enabled the Profiling Of Cellular Responssupporting

confidence: 67%

A Novel Differential Ion Mobility Device Expands the Depth of Proteome Coverage and the Sensitivity of Multiplex Proteomic Measurements

Pfammatter

Bonneil

McManus

et al. 2018

Molecular & Cellular Proteomics

121

142

View full text Add to dashboard Cite

show abstract

“…Making use of shorter wavelengths (760 or 920 nm) should reduce the thermal load by at least one order of magnitude. Although heat-driven damage seems not to be a factor of concern, assuming the previous arguments, it is nevertheless important to be aware that some biological effects can have a thermal origin in some redox experiments using light [223], [224], [225], [226], [227], [228], [229]. Under certain circumstances, ironically, thermal damage can be an asset.…”

Section: Discussionmentioning

confidence: 99%

Direct 1O2 optical excitation: A tool for redox biology

Blázquez‐Castro

2017

Redox Biology

View full text Add to dashboard Cite

Molecular oxygen (O2) displays very interesting properties. Its first excited state, commonly known as singlet oxygen (1O2), is one of the so-called Reactive Oxygen Species (ROS). It has been implicated in many redox processes in biological systems. For many decades its role has been that of a deleterious chemical species, although very positive clinical applications in the Photodynamic Therapy of cancer (PDT) have been reported. More recently, many ROS, and also 1O2, are in the spotlight because of their role in physiological signaling, like cell proliferation or tissue regeneration. However, there are methodological shortcomings to properly assess the role of 1O2 in redox biology with classical generation procedures. In this review the direct optical excitation of O2 to produce 1O2 will be introduced, in order to present its main advantages and drawbacks for biological studies. This photonic approach can provide with many interesting possibilities to understand and put to use ROS in redox signaling and in the biomedical field.

show abstract

“…were consistent with the established role of mitochondria as key sources of ROS generation through the electron transport chain 61. Several lines of evidence have indicated that heat stress can induce ROS production in different cell types 20,25,51. The precise mechanisms of how the temperature increases mitochondrial ROS levels remain undetermined.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescent staining was performed after fixation with 4% paraformaldehyde. Procedures involving fluorescence staining were performed in accordance with the manufacturers’ instructions and methods described previously 25,27,35…”

Section: Methodsmentioning

confidence: 99%

The Effect of Co0.2Mn0.8Fe2O4 Ferrite Nanoparticles on the C2 Canine Mastocytoma Cell Line and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) Cultured Under a Static Magnetic Field: Possible Implications in the Treatment of Dog Mastocytoma

et al. 2017

View full text Add to dashboard Cite

Cobalt manganese ferrite nanoparticles have application potential in the biomedical field, however there is limited information concerning the biological response. The aim of this work was to investigate the cytotoxic potential of cobalt-manganese ferrite nanoparticles in canine mastocytoma tumor cells (C2) and adipose-derived mesenchymal stromal stem cells (ASCs) cultured under a static magnetic field (MF). In this study, we investigated the viability and proliferation rate of ASC and C2 cells cultured with Co0.2Mn0.8Fe2O4 nanoparticles under 0.5T MF. We observed cells morphology and measured intracellular ROS generation. Thermal observations were used to characterize the thermotrophic cell behavior in different condition and RNA level of heat shock proteins and apoptotic genes was measured. Nanoparticles reduced cell viability, caused cell damage, i.e., through the formation of reactive oxygen species (ROS) and increased transcriptional level of apoptotic genes (Bcl-2, Bax, p53, p21). In addition, we have found that C2 mastocytoma cells cultured with metal oxide nanoparticles under MF exhibited unexpected biological responses, including thermotolerance and apoptotic response induced by the expression of heat shock proteins and ROS produced under a MF. Our results suggest that stimulation using MF and Co0.2Mn0.8Fe2O4 nanoparticles is involved in mechanisms associated with controlling cell proliferative potential signaling events. We can state that significant differences between normal and cancer cells in response to nanoparticles and MF are apparent. Our results show that nanoparticles and MF elevate the temperature in vitro in tumor cells, thereby increasing the expression of ROS as well as heat shock proteins.Electronic supplementary materialThe online version of this article (doi:10.1007/s12195-017-0480-0) contains supplementary material, which is available to authorized users.

show abstract

Effect of heat stress-induced production of mitochondrial reactive oxygen species on NADPH oxidase and heme oxygenase-1 mRNA levels in avian muscle cells

Cited by 51 publications

References 42 publications

A Novel Differential Ion Mobility Device Expands the Depth of Proteome Coverage and the Sensitivity of Multiplex Proteomic Measurements

A Novel Differential Ion Mobility Device Expands the Depth of Proteome Coverage and the Sensitivity of Multiplex Proteomic Measurements

Direct 1O2 optical excitation: A tool for redox biology

The Effect of Co0.2Mn0.8Fe2O4 Ferrite Nanoparticles on the C2 Canine Mastocytoma Cell Line and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) Cultured Under a Static Magnetic Field: Possible Implications in the Treatment of Dog Mastocytoma

Contact Info

Product

Resources

About