Effect of heat stress on oxidative damage and antioxidant defense system in white clover (Trifolium repens L.)

Liu, Hsiang Lin; Lee, Zhu-Xuan; Chuang, Tzu-Wei; Wu, Hui-Chen

doi:10.1007/s00425-021-03751-9

Cited by 22 publications

(13 citation statements)

References 84 publications

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“…The expression level of genes related to polyamine oxidase was significantly increased by HD treatment, and the expression level was increased and decreased by HDS treatment. The changes in the gene expression of polyamine anabolic enzymes further verified that the changes in enzyme activity led to the accumulation of polyamine content, which in turn improved the heat tolerance of lettuce, consistent with previous findings in cucumber, where overexpression of polyamine oxidase increased salt tolerance in cucumber seedlings [ 39 ].…”

Section: Discussionsupporting

confidence: 89%

“…MDA is a product of unsaturated fatty acid peroxidation and is a marker of free radical damage in cell membranes [ 38 ]. The maintenance of fatty acid unsaturation plays an important role in the response to environmental stress, and the exogenous application of polyamines reduces lipid peroxidation and ion leakage produced by environmental stress [ 36 , 39 ]. There is evidence that exogenous application of Spd under drought stress attenuated the damage caused by MDA in tea trees, while exogenous polyamine inhibitors significantly increased MDA levels [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

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Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

Hao

et al. 2022

Plants

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High temperature is a huge threat to lettuce production in the world, and spermidine (Spd) has been shown to improve heat tolerance in lettuce, but the action mechanism of Spd and the role of polyamine metabolism are still unclear. The effects of Spd and D-arginine (D-arg) on hydroponic lettuce seedlings under high-temperature stress by foliar spraying of Spd and D-arg were investigated. The results showed that high-temperature stress significantly inhibited the growth of lettuce seedlings, with a 33% decrease in total fresh weight and total dry weight; photosynthesis of lettuce seedlings was inhibited by high-temperature stress, and the inhibition was greater in the D-arg treatment, while the Spd recovery treatment increased net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), stomatal limit value (Ls), and intercellular CO2 concentration (Ci). High-temperature stress significantly reduced the maximum photochemical efficiency (Fv/Fm), photochemical quenching coefficient (qP), electron transport rate (ETR), and photochemical efficiency of PSII (ΦPSII), increased the non-photochemical burst coefficient (NPQ) and reduced the use of light energy, which was alleviated by exogenous Spd. The increase in polyamine content may be due to an increase in polyamine synthase activity and a decrease in polyamine oxidase activity, as evidenced by changes in the expression levels of genes related to polyamine synthesis and metabolism enzymes. This evidence suggested that D-arg suppressed endogenous polyamine levels in lettuce and reduced its tolerance, whereas exogenous Spd promoted the synthesis and accumulation of polyamines in lettuce and increased its photosynthetic and oxidative stress levels, which had an impact on the tolerance of lettuce seedlings.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

Hao

et al. 2022

Plants

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“…High temperature is one of the major abiotic stresses that pose growing and serious challenges to plant growth and development [ 9 ]. Heat stress affects several physiological processes [ 10 ], e.g., it could alter membrane permeability and fluidity, influencing cellular homeostasis [ 11 , 12 ], and cause denaturation and aggregation of proteins [ 13 ], cell damage and ion leakage, interfering with important processes such as respiration and photosynthesis [ 14 ]. Indeed, the photosynthetic apparatus is highly sensitive to high temperatures [ 15 ], namely at the PSII level [ 9 ], associated with the dissociation of the D1 protein, but the electron transport chain (ETC) and the oxygen-evolving complex (OEC), and photosynthetic enzymes, such as RuBisCO activase, could also be inactivated [ 10 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…The reactive oxygen species (ROS), mainly produced in chloroplasts, mitochondria, and peroxisomes [ 23 ], are harmful oxidants able to cause lipid peroxidation, enzyme inactivation, and degradation of pigments, proteins, and DNA [ 24 ] leading ultimately to cell apoptosis [ 25 ]. The control of highly reactive molecules of Chl and O 2 is achieved by promoting energy dissipation mechanisms that prevent its formation (e.g., photoprotective pigments) and the expression of enzymatic and non-enzymatic antioxidants that scavenge the ROS already produced [ 13 , 24 ]. Therefore, the reinforcement of mechanisms dedicated to preventing ROS overproduction and/or its efficient scavenging is usually crucial to plant tolerance to a wide number of environmental constraints.…”

Section: Introductionmentioning

confidence: 99%

Protective Responses at the Biochemical and Molecular Level Differ between a Coffea arabica L. Hybrid and Its Parental Genotypes to Supra-Optimal Temperatures and Elevated Air [CO2]

Vinci

Marques²,

Rodrigues³

et al. 2022

Plants

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Climate changes with global warming associated with rising atmospheric [CO2] can strongly impact crop performance, including coffee, which is one of the most world’s traded agricultural commodities. Therefore, it is of utmost importance to understand the mechanisms of heat tolerance and the potential role of elevated air CO2 (eCO2) in the coffee plant response, particularly regarding the antioxidant and other protective mechanisms, which are crucial for coffee plant acclimation. For that, plants of Coffea arabica cv. Geisha 3, cv. Marsellesa and their hybrid (Geisha 3 × Marsellesa) were grown for 2 years at 25/20 °C (day/night), under 400 (ambient CO2, aCO2) or 700 µL (elevated CO2, eCO2) CO2 L−1, and then gradually submitted to a temperature increase up to 42/30 °C, followed by recovery periods of 4 (Rec4) and 14 days (Rec14). Heat (37/28 °C and/or 42/30 °C) was the major driver of the response of the studied protective molecules and associated genes in all genotypes. That was the case for carotenoids (mostly neoxanthin and lutein), but the maximal (α + β) carotenes pool was found at 37/28 °C only in Marsellesa. All genes (except VDE) encoding for antioxidative enzymes (catalase, CAT; superoxide dismutases, CuSODs; ascorbate peroxidases, APX) or other protective proteins (HSP70, ELIP, Chape20, Chape60) were strongly up-regulated at 37/28 °C, and, especially, at 42/30 °C, in all genotypes, but with maximal transcription in Hybrid plants. Accordingly, heat greatly stimulated the activity of APX and CAT (all genotypes) and glutathione reductase (Geisha3, Hybrid) but not of SOD. Notably, CAT activity increased even at 42/30 °C, concomitantly with a strongly declined APX activity. Therefore, increased thermotolerance might arise through the reinforcement of some ROS-scavenging enzymes and other protective molecules (HSP70, ELIP, Chape20, Chape60). Plants showed low responsiveness to single eCO2 under unstressed conditions, while heat promoted changes in aCO2 plants. Only eCO2 Marsellesa plants showed greater contents of lutein, the pool of the xanthophyll cycle components (V + A + Z), and β-carotene, compared to aCO2 plants at 42/30 °C. This, together with a lower CAT activity, suggests a lower presence of H2O2, likely also associated with the higher photochemical use of energy under eCO2. An incomplete heat stress recovery seemed evident, especially in aCO2 plants, as judged by the maintenance of the greater expression of all genes in all genotypes and increased levels of zeaxanthin (Marsellesa and Hybrid) relative to their initial controls. Altogether, heat was the main response driver of the addressed protective molecules and genes, whereas eCO2 usually attenuated the heat response and promoted a better recovery. Hybrid plants showed stronger gene expression responses, especially at the highest temperature, when compared to their parental genotypes, but altogether, Marsellesa showed a greater acclimation potential. The reinforcement of antioxidative and other protective molecules are, therefore, useful biomarkers to be included in breeding and selection programs to obtain coffee genotypes to thrive under global warming conditions, thus contributing to improved crop sustainability.

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“…The economically important forage, white clover, is vulnerable to abiotic stresses, adversely affecting its normal biological and physiological activities and interrupting fundamental cellular structures [ 29 , 30 ]. Therefore, it is important to continuously explore effective means and resources to improve white clover’s biotic and abiotic stress tolerance.…”

Section: Introductionmentioning

confidence: 99%

A Heat Shock Transcription Factor TrHSFB2a of White Clover Negatively Regulates Drought, Heat and Salt Stress Tolerance in Transgenic Arabidopsis

Iqbal

Jia

Tang

et al. 2022

IJMS

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Heat shock transcription factors (HSF) are divided into classes A, B and C. Class A transcription factors are generally recognized as transcriptional activators, while functional characterization of class B and C heat shock transcription factors have not been fully developed in most plant species. We isolated and characterized a novel HSF transcription factor gene, TrHSFB2a (a class B HSF) gene, from the drought stress-sensitive forage crop species, white clover (Trifolium repens). TrHSFB2a was highly homologous to MtHSFB2b, CarHSFB2a, AtHSFB2b and AtHSFB2a. The expression of TrHSFB2a was strongly induced by drought (PEG6000 15% w/v), high temperature (35 °C) and salt stresses (200 mM L−1 NaCl) in white clover, while subcellular localization analysis showed that it is a nuclear protein. Overexpression of the white clover gene TrHSFB2a in Arabidopsis significantly reduced fresh and dry weight, relative water contents (RWC), maximum photosynthesis efficiency (Fv/Fm) and performance index on the absorption basis (PIABS), while it promoted leaf senescence, relative electrical conductivity (REC) and the contents of malondialdehyde (MDA) compared to a wild type under drought, heat and salt stress conditions of Arabidopsis plants. The silencing of its native homolog (AtHSFB2a) by RNA interference in Arabidopsis thaliana showed opposite trends by significantly increasing fresh and dry weights, RWC, maximum photosynthesis efficiency (Fv/Fm) and performance index on the absorption basis (PIABS) and reducing REC and MDA contents under drought, heat and salt stress conditions compared to wild type Arabidopsis plants. These phenotypic and physiological indicators suggested that the TrHSFB2a of white clover functions as a negative regulator of heat, salt and drought tolerance. The bioinformatics analysis showed that TrHSFB2a contained the core B3 repression domain (BRD) that has been reported as a repressor activator domain in other plant species that might repress the activation of the heat shock-inducible genes required in the stress tolerance process in plants. The present study explores one of the potential causes of drought and heat sensitivity in white clover that can be overcome to some extent by silencing the TrHSFB2a gene in white clover.

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Effect of heat stress on oxidative damage and antioxidant defense system in white clover (Trifolium repens L.)

Cited by 22 publications

References 84 publications

Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

Role of Spermidine in Photosynthesis and Polyamine Metabolism in Lettuce Seedlings under High-Temperature Stress

Protective Responses at the Biochemical and Molecular Level Differ between a Coffea arabica L. Hybrid and Its Parental Genotypes to Supra-Optimal Temperatures and Elevated Air [CO2]

A Heat Shock Transcription Factor TrHSFB2a of White Clover Negatively Regulates Drought, Heat and Salt Stress Tolerance in Transgenic Arabidopsis

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